Gracilaria lemaneiformis agar oligosaccharide and preparation method and application thereof
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A technology of agar oligosaccharide and asparagus, applied in the field of oligosaccharides, can solve the problems of lack, consumption, pollution of water resources, etc., and achieve the effects of good practicability, cost reduction and simple process
Active Publication Date: 2012-12-19
THIRD INST OF OCEANOGRAPHY STATE OCEANIC ADMINISTATION
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However, both the chemical method and the enzymatic hydrolysis method have relatively serious shortcomings, including: ①These methods can only act on seaweed polysaccharides such as agar, and the extraction of seaweed polysaccharides from seaweed requires repeated treatment with alkali and acid and repeated washing. Caused serious pollution and consumption of water resources; ② enzymatic hydrolysis requires large-scale preparation of enzyme preparations before it can be applied to the degradation of polysaccharides
However, the preparation of enzyme preparations has high requirements for instr...
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Embodiment 1
[0027] The microbial fermentation method preparation of embodiment 1 asparagus agar oligosaccharide
[0028] 1) Prepare the strain Flammeovirga Pacifica H2 with activation medium (containing 1% asparagus powder sieved through 60-80 meshes, 0.2% yeast extract, 3% NaCl, 1.5% agar powder, and distilled water. , pH7~8, used after sterilizing at 121°C for 20 minutes) After activation, pick a single colony and inoculate it in the seed medium (containing CMC-Na 2 1%, yeast extract 0.2%, NaCl 3%, distilled water, pH 7-8, sterilized at 121°C for 20 minutes before use), shaking culture at 30°C until OD 600 = 1.0;
[0029] 2) Add the fermentation broth to the sugar production medium (containing 3% asparagus powder sieved through 60-80 meshes, MgCl 2 0.25%, prepared with distilled water, pH 7-8, sterilized at 121°C for 20 minutes before use), continue to shake and ferment at 30°C for 48 hours;
[0030] 3) Centrifuge the fermentation broth at 10,000×g for 15 minutes, and collect the su...
Embodiment 2
[0072] Similar to Example 1, the difference is that the culture temperature in steps 1) and 2) is 25°C, and the CMC-Na in the seed medium in step 1) 2 The content of asparagus is 0.5%, step 2) asparagus powder is 1%, MgCl 2 The content is 0.1%, and the incubation time is 72h.
Embodiment 3
[0074] Similar to Example 1, the difference is that the culture temperature in steps 1) and 2) is 37°C, and the CMC-Na in the seed medium in step 1) 2 The content of asparagus is 1.5%, the asparagus powder in step 2) is 5%, MgCl 2 The content is 0.5%, and the incubation time is 24h.
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Abstract
The invention discloses a gracilaria lemaneiformis agar oligosaccharide and the preparation method and application thereof and relates to an oligosaccharide. The invention provides a gracilaria lemaneiformis agar oligosaccharide, the preparation method of gracilaria lemaneiformis agar oligosaccharide and the application of the gracilaria lemaneiformis agar oligosaccharide in preparation of antioxidant and uvioresistant health care products and cosmetics. Pacific heating color bacillus H2 and gracilaria lemaneiformis are co-cultivated and the gracilaria lemaneiformis is directly degraded by the enzyme system which is generated by bacterial strain to obtain the gracilaria lemaneiformis agar oligosaccharide of which the degree of polymerization is 4-8. The gracilaria lemaneiformis agar oligosaccharide has the physiological activities of antioxidation, absorbing ultraviolet rays and the like. In preparation, the bacterial strain is activated by an activation medium plate and inoculated in a seed medium; fermentation broth is obtained by shake cultivation and inoculated in a saccharide generating medium; after shaking fermental cultivation and centrifugalization, supernate is collected; the supernate is successively filtered by a membrane filtration devices with a 0.45mu m filter membrane, a 0.22mu m filter membrane, a 10000-50000D ultrafiltration membrane and a 300-600D ultrafiltration membrane, and the percolate is the gracilaria lemaneiformis agar oligosaccharide.
Description
technical field [0001] The invention relates to an oligosaccharide, in particular to an asparagus agar oligosaccharide and a preparation method thereof. The oligosaccharide is prepared by co-cultivating a bacterial strain and asparagus, and has physiological activities such as anti-oxidation and anti-ultraviolet rays. Background technique [0002] Oligosaccharides refer to straight-chain or branched low-polymerized sugar substances composed of 2 to 20 identical or different monosaccharides, also known as oligosaccharides, including two categories: ordinary oligosaccharides and functional oligosaccharides. Common oligosaccharides include sucrose, maltose, and lactose, which are familiar to people. These sugars can be digested and absorbed by the body, and have no growth-promoting effect on intestinal beneficial bacteria. Xylose, soybean oligosaccharides, chitooligosaccharides, agar oligosaccharides, algin oligosaccharides, etc., cannot be digested and absorbed by the body, bu...
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