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Pediococcus pentosaceus having good resistance to oxidation and whitening skin and application thereof

A technology of Pediococcus pentosaceae and whitening effect, which is applied in the direction of bacteria, cosmetics, cosmetic preparations, etc., can solve the problems that natural skin care products cannot meet consumer demand, and the development is not strong enough to achieve the promotion of DPPH clearance rate and tyrosine Enzyme inhibition rate, excellent effect, effect of good effect

Active Publication Date: 2020-04-07
SHANGHAI INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Due to the fact that the current domestic market does not have a lot of development efforts in the integration of probiotic fermentation into skin care, natural skin care products are still dominated by plant extracts. The leading natural skin care products have gradually failed to meet people's consumption needs

Method used

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  • Pediococcus pentosaceus having good resistance to oxidation and whitening skin and application thereof
  • Pediococcus pentosaceus having good resistance to oxidation and whitening skin and application thereof
  • Pediococcus pentosaceus having good resistance to oxidation and whitening skin and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Antioxidant determination of embodiment 1 lactic acid bacteria extracellular active substance

[0031] (1) Take the alcoholic medicine sample to be tested, add sterile water, dilute 10 times in a gradient, oscillate, take the supernatant and spread it on a solid medium, cultivate it for 12-48 hours, and obtain a single colony as the strain to be screened;

[0032] (2) Under sterile conditions, take the bacterial strain to be screened, inoculate it in a multi-hole cell culture plate containing a liquid medium, and activate it for 3 generations before use;

[0033] (3) After centrifuging the activated bacterial solution at 4000r / min for 2min, take the supernatant for later use;

[0034] (4) Carry out the pre-test, take 2 mL of DPPH solution, add a small amount of sample solution to it, when adding the sample, gradually add less and then more, mix while adding, and observe the fading of the solution, when the color of the solution basically fades, record The sample volume...

Embodiment 2

[0042] The tyrosinase inhibitory activity assay of embodiment 2 lactic acid bacteria extracellular active substances

[0043] (1) Get the 30 bacterial strains to be tested screened in Example 1, after the activated bacterial liquid is centrifuged at 4000r / min for 2min, get the supernatant for later use;

[0044] (2) Configure the required solution according to the table below:

[0045] Table 2. Determination of tyrosinase inhibition rate experimental sample addition table

[0046]

[0047]

[0048] Add C1, C2, T1, and T2 according to the above table and bathe in a water bath at 37°C for 10 minutes, add 1 mL of dopa solution and bathe in a water bath at 37°C for 4 minutes, and immediately put it into a spectrophotometer at 475nm to measure the absorbance at the 5th minute , Calculate the inhibition rate of the sample to tyrosinase activity T (%)=1-(T1-T2) / (C1-C2)×100%.

[0049] (3) The experimental results are listed in Table 3. It can be seen from the table that strai...

Embodiment 3

[0052] Antioxidant activity, tyrosinase inhibition rate determination of embodiment 3 lactic acid bacteria intracellular active substance

[0053] (1) Take the strain 5-4 to be tested, centrifuge the activated bacterial solution at 4000r / min for 2min, and then centrifuge the supernatant (that is, the mixture of MRS medium and extracellular secretion) in a sterile operating table. completely empty;

[0054] (2) Add 0.9ml of sterile water, use a micro-vortex mixer to mix it completely, cover the EP tube, and centrifuge again at low temperature and low speed for a short period of time. The purpose of this centrifugation is to remove the remaining EP tube. The mixture of MRS medium and extracellular secretions, repeat this experimental step 2-3 times until the medium in the EP tube is completely washed;

[0055] (3) Find a small glass beaker, add an appropriate amount of ice-water mixture and a small foam orifice to it, open the EP tube and insert it into the small foam orifice, ...

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Abstract

The invention provides pediococcus pentosaceus and the accession number of the pediococcus pentosaceus is CCTCC M 2019323. Tests prove that the DPPP free radical scavenging rate of an extracellular supernatant of the pediococcus pentosaceus CCTCC M 2019323 is 81.89% and the tyrosinase inhibition rate is 64.82%. The extracellular supernatant of the pediococcus pentosaceus CCTCC M 2019323 is fermented by taking a medicinal plant as a matrix and the fermentation liquor has a good propionibacterium acnes inhibiting effect. Meanwhile, the medicinal plant fermentation liquor can also be applied to preparation of cosmetics, and the prepared plant fermentation liquor product containing the pediococcus pentosaceus CCTCC M 2019323 can effectively remove free radicals, promote skin renewal and reducesynthesis of melanin. The pediococcus pentosaceus has the broad application prospects.

Description

technical field [0001] The invention relates to the screening and identification of lactic acid bacteria with high oxidation resistance and whitening effect and its application in cosmetics, belonging to the field of biotechnology. Background technique [0002] Skin care products have always been a hot topic in consumption, and consumers' desire for excellence in skin care has promoted the vigorous development of the market. It can be seen from the market trend in recent years that the growth of natural plant cosmetics has always exceeded that of synthetic cosmetics globally, and China is the fastest growing country for natural plant cosmetics. In addition to the use of foreign popular plant ingredients by international brands, It has also begun to intervene in the biological field. The current popular SK-II fairy water has a good whitening effect because it is rich in yeast fermentation liquid. Microbial fermentation technology is the process of using the metabolism of mic...

Claims

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Application Information

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IPC IPC(8): C12N1/20A61K8/99A61Q19/02A61Q19/08C12R1/01
CPCC12N1/20A61K8/99A61Q19/02A61Q19/08C12R2001/01C12N1/205
Inventor 陈臣刘洋于海燕田怀香袁佳杰杨茹梦郭伟陈霜
Owner SHANGHAI INST OF TECH
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