The invention discloses a micropterus salmoides immune nutritional functional feed composition and a preparation method thereof. The micropterus salmoides immune nutritional functional feed composition comprises the following main raw materials: fish meal, flour, whole shrimp meal and wheat gluten. The process is simple, fish meal, flour, whole shrimp meal, wheat gluten, beer yeast, fish oil, soybean lecithin, monocalcium phosphate, chicken meal, minerals, a phagostimulant, vitamin C, astragalus polysaccharide, glycyrrhiza polysaccharide, haematococcus pluvialis and cordyceps militaris are utilized, nutrient substances with different characteristics are optimized, compounded and combined, and the immune nutritional functional feed is produced. The feed has the effects of being rich in nutrition, clean, sanitary and capable of increasing the yield of aquatic products, and particularly has an excellent effect of improving the immunity of organisms; and moreover, the feed is more sanitary in preparation mode, low in equipment requirement and high in operability, has good social popularization and application, can remarkably promote growth and shorten the breeding period, and effectively improves the natural immunity and the anti-stress capability of micropterus salmoides.