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Application of curdlan oligomers

A technology of Cortlan oligosaccharides and oligosaccharides, applied in the fields of application, chemicals for biological control, animal repellents, etc. Problems such as high technical difficulty, prohibitive difficulty and cost, to achieve the effect of enhancing natural immunity, inducing plant disease resistance, and avoiding side effects

Inactive Publication Date: 2012-01-11
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the chemical synthesis of cotlan oligosaccharides is difficult, especially the synthesis of oligosaccharide monomers with DP>7, because it involves a series of problems such as the connection mode and three-dimensional configuration of sugar units, and the protection of active groups. and cost are prohibitive
[0005] If there is no effective method for preparing Cortlan oligosaccharides, it will not be conducive to the study of the structure and biological activity of oligosaccharides, and it will hinder the in-depth exploration of the relationship between Cortlan oligosaccharides and their derivatives and the occurrence of diseases from the perspective of mechanism

Method used

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  • Application of curdlan oligomers
  • Application of curdlan oligomers
  • Application of curdlan oligomers

Examples

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Effect test

preparation example Construction

[0027] The preparation method (A) of Cortlan oligosaccharide

[0028] Weigh 5g of candlan polysaccharide in 50ml of potassium iodide and cadmium ethylenediamine solution (ratio: 1:1) and stir well until completely dissolved, slowly add 0.5 M oxalic acid mixed solution 3ml, reacted for 60min, precipitated with 50mL of ethanol and acetone mixed solution (V / V=1), while stirring, to obtain milky white powder, suction filtered, and vacuum dried at 50°C to obtain 4.25g, yield up to 85.12%.

[0029] It was proved by high performance anion exchange chromatography pulsed amperometry and matrix-assisted laser desorption ionization time-of-flight mass spectrometry that the obtained substance was Cortlan oligosaccharide.

[0030] The preparation method (B) of Cortlan oligosaccharide

[0031] Weigh 5g of candlan polysaccharide in 50ml of dimethyl sulfoxide and potassium iodide solution (ratio: 1:1), stir well until completely dissolved, and slowly add 3ml of 0.5M trifluoroacetic acid mi...

Embodiment 1

[0032] Embodiment 1: Experiment of inducing plant disease resistance

[0033] Materials and methods: Cotland oligosaccharides and chitosan oligosaccharides; the tested plant was Nicotiana tobaccoum L.SamSun NN; the tested virus source was tobacco mosaic virus (TMV)

[0034] Greenhouse test: control agent chitosan oligosaccharide 50μg / ml, spray. The concentrations of the test agent candlan polysaccharide mixture were sprayed at 1 μg / mL, 10 μg / mL, 50 μg / mL, 100 μg / mL and 500 μg / mL, respectively. Tobacco plants at the 6-8 leaf stage of the same size were selected, and sprayed on the leaves, 10 mL per plant. After 24 hours the sap was inoculated with TMV virus. Add a small amount of quartz sand to the virus juice, dip the juice with a brush and rub the inoculation. Blighted Sansheng tobacco seedlings are inoculated with the half-leaf method, and each plant is inoculated with four leaves. The incidence of disease was observed every day after inoculation. After the disease is f...

Embodiment 2

[0035] Embodiment 2: experiment of promoting plant growth

[0036] After 15%H 2 o 2 (wt / wt) solution soaked sterilized corn seeds in 0.2M CaCl 2Soak in medium for 30min to increase the germination rate of the seeds, and germinate on a petri dish with moist filter paper in a dark environment. Two days later, the seeds germinated and cotyledons appeared, which were transferred to a container of hydroponic nutrient solution, and fresh nutrient solution was replaced every day. After the corn grows into seedlings, make the test agent cotland oligosaccharide into an aqueous solution with a concentration of 0.01-10%, adjust the pH to 6 to 7, and replace the fresh hydroponic nutrient solution containing the oligosaccharide sample every day, without adding the oligosaccharide sample The hydroponic nutrient solution was used as a blank control. The experimental results showed that codran oligosaccharides increased the corn biomass by 15%, and the chlorophyll content increased by 18....

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Abstract

The invention discloses an application of curdlan oligomers. The curdlan oligomers have the structure of oligomers formed by connecting glucose molecules through beta-(1->3) glucosidic bonds, and the degree of polymerization (Degree of Polymerization, DP) is 2-24, and the molecular formula is (C6H12O6)n, wherein n=2-24; and the average molecular weight of the curdlan oligomers ranges from 340 Da to 4000 Da. The curdlan oligomers prepared in the invention can be used as green biological pesticides for adjusting the growth of plants and inducing the resistance of the plants.

Description

technical field [0001] The present invention relates to cotlan oligosaccharides, in particular to the application of cotlan oligosaccharides, which can induce plant resistance and promote plant growth and development. Background technique [0002] Cordlan polysaccharide is an exopolysaccharide produced by the fermentation of Alcaligenes faecalis var myxogenes 10c3. Its basic structure is glucose connected by β-(1→3) glycosidic bonds. It is a member of the β-1,3-glucan family member. So far, this polysaccharide is the only non-branched β-1,3-glucan in nature. However, its application in food, medicine, feed additives and materials is seriously hindered due to its insolubility in water. [0003] Cotlan oligosaccharides, also known as β-1,3-glucooligosaccharides, are degradation products of polysaccharides, and their water solubility is greatly enhanced. As the research on oligosaccharides has attracted more and more attention, the biological functions and physiological acti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N43/16A01P1/00A01P3/00A01P21/00
Inventor 杜昱光傅赟彬刘启顺赵小明白雪芳尹恒王文霞
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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