A kind of chitosan-modified nano-enzyme mucosal immune adjuvant and influenza mucosal vaccine and preparation method thereof

An immune adjuvant and mucosal vaccine technology, applied in the field of vaccines, to achieve the effects of high biocompatibility, improved efficiency, and significant antigen-targeted mucosal delivery capabilities

Active Publication Date: 2022-08-05
YANGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no relevant report on the application of IONzyme in mucosal adjuvants

Method used

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  • A kind of chitosan-modified nano-enzyme mucosal immune adjuvant and influenza mucosal vaccine and preparation method thereof
  • A kind of chitosan-modified nano-enzyme mucosal immune adjuvant and influenza mucosal vaccine and preparation method thereof
  • A kind of chitosan-modified nano-enzyme mucosal immune adjuvant and influenza mucosal vaccine and preparation method thereof

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Experimental program
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preparation example Construction

[0056] The invention provides a chitosan-modified nano-enzyme mucosal immune adjuvant, which is prepared by the following steps:

[0057] The ethylene glycol solution of anhydrous ferric chloride is mixed with sodium acetate trihydrate, the obtained suspension is mixed with chitosan, and then heated at 198 to 202 ° C for 11 to 13 hours, and the obtained black precipitate is chitosan modified the nanozyme;

[0058] The mass ratio of the ferric chloride and chitosan is (0.83-8.2):1.

[0059] In the present invention, the preparation method of the chitosan-modified nanozyme is prepared by a hydrothermal synthesis method. The temperature of the heating reaction is preferably 200° C., and the time of the heating reaction is preferably 12 h. The anhydrous ferric chloride is dissolved in the ethylene glycol solution, preferably by magnetic stirring to completely dissolve the anhydrous ferric chloride in the ethylene glycol. The mass ratio of the anhydrous ferric chloride to the vo...

Embodiment 1

[0079] Establishment and functional evaluation of cross-linking method of chitosan-nanozyme-inactivated influenza virus

[0080] 1.1 Synthesis of chitosan-modified nanozymes

[0081] Chitosan-Iron oxide nanozyme (CS-IONzyme) was prepared by hydrothermal synthesis. First, 0.82 g of anhydrous ferric chloride was completely dissolved in 40 mL of ethylene glycol by magnetic stirring to form a clear solution. Next, 3.6 g of sodium acetate trihydrate was slowly added and stirred rapidly to form a uniform suspension. Then add 0.1 g of chitosan (Chitosan, CS) with different molecular weights, including low molecular weight (50-190KDa), medium molecular weight (190-310KDa), high molecular weight (310-375KDa), after fully stirring, ultrasonic for 10min to make the shell The polysaccharide is uniformly mixed with the above solution. Then, the solution was transferred to a 50 mL polytetrafluoroethylene reactor, and the reactor was heated at 200° C. for 12 h. After the reaction kettle ...

Embodiment 2

[0085] 1.1 Synthesis of chitosan-modified nanozymes

[0086] Chitosan-Iron oxide nanozyme (CS-IONzyme) was prepared by hydrothermal synthesis. First, 8.3 g of anhydrous ferric chloride was completely dissolved in 40 mL of ethylene glycol by magnetic stirring to form a clear solution. Next, 3.7 g of sodium acetate trihydrate was slowly added and stirred rapidly to form a uniform suspension. Then add 1.0 g of chitosan (Chitosan, CS) with different molecular weights, including low molecular weight (50-190KDa), medium molecular weight (190-310KDa), high molecular weight (310-375KDa), after fully stirring, ultrasonic for 10min to make the shells The polysaccharide is uniformly mixed with the above solution. Then, the solution was transferred to a 50 mL polytetrafluoroethylene reactor, and the reactor was heated at 200° C. for 12 h. After the reaction kettle was naturally cooled to room temperature, a black precipitate was obtained, that is, the chitosan-modified nanozyme. Then,...

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Abstract

The invention provides a chitosan-modified nano-enzyme mucosal immune adjuvant, an influenza mucosal vaccine and a preparation method thereof, belonging to the technical field of vaccines. The chitosan-modified nanozyme can activate the ROS level of dendritic cells by enzymatic catalysis to enhance the level of innate immunity. It has significant mucosal immune adjuvant properties, and can also increase the number of influenza viruses adhered to the mucosa and prolong the adhesion time. After cross-linking and inactivating influenza virus intranasal immunization with chitosan-modified nanozymes, the specific mucosal immunity and systemic immunity levels against influenza virus were significantly enhanced, and it had significant antigen-targeted mucosal delivery ability and mucosal adjuvant properties. The prepared mucosal vaccine can completely protect mice from lethal challenge to influenza virus after two intranasal immunizations, and has broad application prospects.

Description

technical field [0001] The invention belongs to the technical field of vaccines, and in particular relates to a chitosan-modified nano-enzyme mucosal immune adjuvant, an influenza mucosal vaccine and a preparation method thereof. Background technique [0002] Influenza, abbreviated as Influenza, is a zoonotic infectious disease caused by Influenza virus. Influenza viruses can be divided into types A, B and C, among which type A is the greatest threat to humans [1]. The virus is mainly transmitted through the respiratory tract, and historical influenza pandemics have caused huge human and economic losses to people all over the world. The Spanish influenza (virus subtype H1N1) outbreak in Europe from 1917 to 1919 caused 50 million deaths and was the most severe influenza epidemic in history [2]. At present, the most commonly used method to prevent and control influenza virus is intramuscular inactivated influenza virus vaccine, including whole virus inactivated influenza vac...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K39/145A61P31/16A61K39/39B82Y5/00
CPCA61K39/12A61P31/16A61K39/39B82Y5/00A61K2039/543A61K2039/55583A61K2039/555A61K2039/5252C12N2760/16134
Inventor 高利增秦涛阴银燕彭大新陈素娟马尚
Owner YANGZHOU UNIV
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