Chitosan-modified nano-enzyme mucosal immune adjuvant, influenza mucosal vaccine and preparation method of influenza mucosal vaccine

An immune adjuvant and mucosal vaccine technology, applied in the field of vaccines, to achieve the effect of improving the level of systemic immune response, improving efficiency and high stability

Active Publication Date: 2020-09-04
YANGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is no relevant report on the application of IONzyme in mucosal adjuvants

Method used

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  • Chitosan-modified nano-enzyme mucosal immune adjuvant, influenza mucosal vaccine and preparation method of influenza mucosal vaccine
  • Chitosan-modified nano-enzyme mucosal immune adjuvant, influenza mucosal vaccine and preparation method of influenza mucosal vaccine
  • Chitosan-modified nano-enzyme mucosal immune adjuvant, influenza mucosal vaccine and preparation method of influenza mucosal vaccine

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Experimental program
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preparation example Construction

[0056] The invention provides a chitosan-modified nanozyme mucosal immune adjuvant, which is prepared by the following steps:

[0057] The ethylene glycol solution of anhydrous ferric chloride is mixed with sodium acetate trihydrate, the obtained suspension is mixed with chitosan, and then heated at 198-202°C for 11-13 hours, the black precipitate obtained is chitosan-modified nanozyme;

[0058] The mass ratio of the ferric chloride to chitosan is (0.83-8.2):1.

[0059] In the present invention, the preparation method of the chitosan-modified nanozyme adopts a hydrothermal synthesis method. The temperature of the heating reaction is preferably 200° C., and the time of the heating reaction is preferably 12 hours. The anhydrous ferric chloride is dissolved in the ethylene glycol solution, preferably the anhydrous ferric chloride is completely dissolved in the ethylene glycol by magnetic stirring. The mass ratio of the anhydrous ferric chloride to the volume ratio of the ethyl...

Embodiment 1

[0079] Establishment and functional evaluation of chitosan-nanozyme-inactivated influenza virus cross-linking method

[0080] 1.1 Synthesis of chitosan-modified nanozymes

[0081] A chitosan-modified nanozyme (Chitosan-Iron oxide nanozyme, CS-IONzyme) was prepared by hydrothermal synthesis. First, 0.82 g of anhydrous ferric chloride was completely dissolved in 40 mL of ethylene glycol by magnetic stirring to form a clear solution. Next, slowly add 3.6g of sodium acetate trihydrate and stir rapidly to form a uniform suspension. Then add 0.1g of chitosan (Chitosan, CS) with different molecular weights, including low molecular weight (50-190KDa), medium molecular weight (190-310KDa), high molecular weight (310-375KDa), after fully stirring, ultrasonic 10min, make the shell The polysaccharide is uniformly mixed with the above solution. Then, the solution was transferred to a 50 mL polytetrafluoroethylene reactor, and the reactor was heated at 200° C. for 12 h. After the reacto...

Embodiment 2

[0085] 1.1 Synthesis of chitosan-modified nanozymes

[0086] A chitosan-modified nanozyme (Chitosan-Iron oxide nanozyme, CS-IONzyme) was prepared by hydrothermal synthesis. First, 8.3 g of anhydrous ferric chloride was completely dissolved in 40 mL of ethylene glycol by magnetic stirring to form a clear solution. Next, slowly add 3.7g of sodium acetate trihydrate and stir rapidly to form a uniform suspension. Then add 1.0 g of chitosan (Chitosan, CS) with different molecular weights, including low molecular weight (50-190KDa), medium molecular weight (190-310KDa), high molecular weight (310-375KDa), after fully stirring, ultrasonic 10min, make the shell The polysaccharide is uniformly mixed with the above solution. Then, the solution was transferred to a 50 mL polytetrafluoroethylene reactor, and the reactor was heated at 200° C. for 12 h. After the reactor was naturally cooled to room temperature, a black precipitate was obtained, which was chitosan-modified nanozyme. The...

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Abstract

The invention provides a chitosan-modified nano-enzyme mucosal immune adjuvant, an influenza mucosal vaccine and a preparation method of the influenza mucosal vaccine, and belongs to the technical field of vaccines. Chitosan-modified nano-enzyme can activate the ROS level of dendritic cells in an enzyme catalysis mode to enhance the natural immune level, has remarkable mucosal immune adjuvant characteristics, and can increase the number of influenza virus adhesion mucosae and prolong the adhesion time. After the chitosan-modified nano-enzyme is cross-linked to inactivate influenza virus nasaldrip immunization, the specific mucosal immunity and systemic immune level for influenza viruses are remarkably enhanced, and the antigen-targeted mucosal delivery capacity and mucosal adjuvant characteristics are remarkable; the prepared mucosal vaccine can completely protect mice from fatal attacks on the influenza viruses after two times of nasal drip immunization, and has wide application prospects.

Description

technical field [0001] The invention belongs to the technical field of vaccines, and in particular relates to a chitosan-modified nano-enzyme mucosal immune adjuvant, an influenza mucosal vaccine and a preparation method thereof. Background technique [0002] Influenza is abbreviated as influenza (Influenza), is a kind of zoonotic infectious disease caused by influenza virus (Influenza virus). Influenza viruses can be divided into types A, B, and C, among which type A is the greatest threat to humans [1]. The virus is mainly transmitted through the respiratory tract. Historically, the pandemic of influenza virus has caused huge losses in personnel and economy to people all over the world. The Spanish flu (virus subtype H1N1) outbreak in Europe from 1917 to 1919 caused 50 million deaths and was the worst influenza epidemic in history [2]. At present, the most commonly used method for preventing and controlling influenza virus is intramuscular injection of influenza virus in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/145A61P31/16A61K39/39B82Y5/00
CPCA61K39/12A61P31/16A61K39/39B82Y5/00A61K2039/543A61K2039/55583A61K2039/555A61K2039/5252C12N2760/16134
Inventor 高利增秦涛阴银燕彭大新陈素娟马尚
Owner YANGZHOU UNIV
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