Microorganism additive agent for improving phylactic power of pets and accelerating growth

A microbial additive, growth-promoting technology, applied in the directions of non-active ingredients of polymer compounds, drug combinations, and medical preparations containing active ingredients, etc. The effect of natural immunity, reducing diarrhea of ​​livestock and poultry, and reducing the viscosity of chyme

Inactive Publication Date: 2008-07-16
SHANGHAI CHUANGBO ECOLOGICAL ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, if the pet's immunity is low, some common diseases will take advantage of it. Because pets have imperfect body temperature regulation function, poor resistance to cold stress, imperfect digestive organs, imperfect digestive gland function, and lack of innate immunity Stress, easy to get sick, pets are prone to enteritis, diarrhea, loss of appetite, indigestion, weak immunity and other diseases under normal feeding conditions

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0039] Step 1: Breeding of Fermentation Strains

[0040] a. Strain subculture: Bacillus subtilis strains, Lactobacillus plantarum and Bifidobacterium provided by the Culture Conservation Center of the Ministry of Agriculture are used to subculture through nutrient agar medium to select and breed strains with fast growth and obvious colony characteristics. Preserve strains at ℃ temperature;

[0041] b. Compound induction of strains: the preserved Bacillus subtilis was diluted to 10 with 0.9% normal saline -5 -10 -7 , treat the bacterial suspension with low heat in a microwave oven for 30S, count by hemocytometer, spread the treated bacterial suspension on a starch medium plate, and culture it upside down at 38°C for 48 hours, and select the cultured one. Colonies with a ratio of the diameter of the transparent circle to the diameter of the colony (HC value) greater than 0.6 are inoculated into nutrient agar test tubes for preservation; the preserved strains are made into a ba...

Embodiment 2

[0061] Step 1: Breeding of Fermentation Strains

[0062] (1) strain passage: with embodiment 1;

[0063] (2) Composite induction of strains: the preserved Bacillus subtilis was diluted to 10 with 0.9% normal saline -5 -10 -7 , treat the bacterial suspension in a microwave oven with low heat for 50 seconds, count by hemocytometer, spread the treated bacterial suspension on a starch medium plate, and invert it for 48 hours at a temperature of 35°C, and select the cultured one. Colonies with a ratio of the diameter of the transparent circle to the diameter of the colony (HC value) greater than 0.6 are inoculated into nutrient agar test tubes for preservation; the preserved strains are made into a bacterial suspension and diluted to 10 -6 , treated with a UV lamp (wavelength: 260nm) with a power of 15W at 30cm for 15s, and the treated bacterial suspension was cultured upside down on a nutrient agar plate at 35°C for 48h, and the colony with a fast growth rate was selected for a ...

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Abstract

The invention relates to a microorganism multienzyme additive for promoting the growth and development of pets, which is characterized in that beneficial strains that are fixedly planted in healthy animal intestinal tract are filtered by microwave and radial-induced technology by adopting co-induction; methods of liquid complex culture and solid amplification culture are adopted, and special enzyme inducer and protein source are added, thereby causing the beneficial strains to amount to 3 billion/g and contain large quantity of physiological metabolites, such as organized eozymes, small peptides, antioxidants, free amino acid, vitamin, etc. The microorganism multienzyme additive of the invention facilitates the pets to be more glossy, massively built, more immune competent and protected from enteric diseases, such as diarrhea, constipation, acute and chronic enteritis, etc., rickets, osteoporosis, etc. Meanwhile, the pollution caused by the excrement of livestock and poultry is relieved; as being free of antibiotic or steroid hormone, the microorganism multienzyme additive is an environmental friendly, safe and novel product of breed technology.

Description

technical field [0001] The invention relates to a microbial additive for improving pet immunity and promoting growth and development, which is a technical product produced by fermentation of various microorganisms to produce multi-enzyme, which can promote pet fur shine, physical fitness, enhance pet immunity, and prevent diarrhea and constipation. Intestinal diseases such as chronic enteritis, rickets, osteoporosis and other diseases; at the same time, reducing the pollution of livestock and poultry manure to the environment belongs to the field of agricultural technology products. Background technique [0002] At present, a Chinese invention patent has been published, the application number is: 200510110758.1, and the name is: "a pharmaceutical composition for pet health care and its preparation method". [0003] The invention belongs to the field of traditional Chinese medicine, and specifically relates to a pharmaceutical composition for health care of animals, pets, dog...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/74A61K47/40A61P37/04A61P43/00C12N1/20A61K35/742A61K35/745A61K35/747
Inventor 江瀚
Owner SHANGHAI CHUANGBO ECOLOGICAL ENG
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