Method for producing xylooligosaccharide under catalysis of xylonic acid

A technology of xylo-oligosaccharides and xylosaccharides, which is applied in the fields of food engineering and chemical engineering, can solve the problems of high cost of biological enzymes, many by-products, and low yield of xylo-oligosaccharides.

Active Publication Date: 2019-12-27
NANJING FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The current production of xylo-oligosaccharides mainly uses endo-xylanase preparations to catalyze the hydrolysis of alkali-extracted xylans, but this method relies on high-priced biological enzymes, high costs, long cycles, and

Method used

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  • Method for producing xylooligosaccharide under catalysis of xylonic acid
  • Method for producing xylooligosaccharide under catalysis of xylonic acid
  • Method for producing xylooligosaccharide under catalysis of xylonic acid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] In a 1L mechanically stirred stainless steel high-pressure reaction tank, add 50g of corncob alkali-extracted xylan and 500mL of 5% (mass fraction) xylose acid solution, seal it, start stirring (50rpm), and heat to 150°C for 75min After the reaction, the reactant tank is down to room temperature, and the reacted solid-liquid mixture is packed into a vacuum pulp washer, and is separated from unhydrolyzed solids and xylan hydrolyzate by extrusion and filtration (hydrolyzate mainly It is a mixture of xylose, xylonic acid and xylooligosaccharide). The obtained xylan hydrolyzate sample was analyzed for its sugar components by high-efficiency anion-exchange chromatography, chromatographic conditions: American Thermo Fisher ICS5000 ion chromatography, equipped with CarboPacTM PA200 (3mm × 250mm) chromatographic column, PAD integral amperometric detector, column The temperature was 30°C, the injection volume was 10 μL; binary gradient elution was performed with 100 mmol / L sodiu...

Embodiment 2

[0031] In a 1L mechanically stirred stainless steel high-pressure reaction tank, add 50g of corncob alkali-extracted xylan and 500mL of 10% (mass fraction) xylose acid solution, seal it, start stirring (50rpm), and heat to 160°C for 45min After the reaction, the reactant tank is down to room temperature, and the reacted solid-liquid mixture is packed into a vacuum pulp washer, and is separated from unhydrolyzed solids and xylan hydrolyzate by extrusion and filtration (hydrolyzate mainly It is a mixture of xylose, xylonic acid and xylooligosaccharide). The obtained xylan hydrolyzate sample was analyzed for its sugar components by high-efficiency anion-exchange chromatography, chromatographic conditions: American Thermo Fisher ICS5000 ion chromatography, equipped with CarboPacTM PA200 (3mm × 250mm) chromatographic column, PAD integral amperometric detector, column The temperature was 30°C, the injection volume was 10 μL; binary gradient elution was performed with 100 mmol / L sodi...

Embodiment 3

[0033]In a 1L mechanically stirred stainless steel high-pressure reaction tank, add 50g of corncobs and 500mL of 5% (mass fraction) xylose acid solution, seal and start stirring (50rpm), and heat to 170°C for 15min; after the reaction, wait for the reaction After the body tank is lowered to room temperature, put the reacted solid-liquid mixture into a vacuum pulp washer, squeeze and filter to separate the unhydrolyzed solids and xylan hydrolyzate (the hydrolyzate is mainly xylose, xylose acid and xylooligosaccharide mixture). The obtained xylan hydrolyzate sample was analyzed for its sugar components by high-efficiency anion-exchange chromatography, chromatographic conditions: American Thermo Fisher ICS5000 ion chromatography, equipped with CarboPacTM PA200 (3mm × 250mm) chromatographic column, PAD integral amperometric detector, column The temperature was 30°C, the injection volume was 10 μL; binary gradient elution was performed with 100 mmol / L sodium hydroxide and 500 mmol / ...

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Abstract

The invention discloses a method for producing xylooligosaccharide under catalysis of xylonic acid. The method for producing the xylooligosaccharide under catalysis of the xylonic acid comprises the following steps: mixing 1 part by mass of a xylan raw material and 5-12 parts by mass of the xylonic acid, and performing heating and stirring. Preferentially, the method for producing the xylooligosaccharide by fermentation under catalysis comprises the following steps: mixing xylose and bacteria, adjusting the pH, and performing stirring at low temperature; and adding the xylan raw material, andperforming heating and stirring. The method utilizes the reaction of microbial whole cell biological oxidation of the xylose to the xylonic acid, and uses the produced xylonic acid as a catalyst, andcompared with a product prepared by using acetic acid and other inorganic acids, the produced glycan prepared by using the xylonic acid is not easily excessively degraded, and has a high yield and less by-product xylose furfural.

Description

technical field [0001] The invention belongs to the technical fields of food engineering and chemical engineering, and in particular relates to a method for producing xylooligosaccharides catalyzed by xylonic acid. Background technique [0002] Xylooligosaccharides, also known as xylooligosaccharides, are mainly derived from the hydrolysis of xylan in wood fibers. As functional food or feed additives, they cannot be absorbed by the digestive system but can selectively proliferate bifidobacteria in the intestinal tract and activate multiple bacteria at the same time. Therefore, under the traction and drive of the rapid development of industries such as human health and microecology, food safety and green animal breeding, and ecological agriculture, xylo-oligosaccharide products derived from wood fibers are used as a "super strong The development prospect of "prebiotics" is very broad. The current production of xylo-oligosaccharides mainly uses endo-xylanase preparations to c...

Claims

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Application Information

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IPC IPC(8): C12P19/00C12P19/02C12P19/04C12P19/12C12R1/01
CPCC12P19/00C12P19/02C12P19/04C12P19/12
Inventor 徐勇周鑫
Owner NANJING FORESTRY UNIV
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