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Plant lactobacillus feed capable of relieving tilapia lead toxicity

A technology of Lactobacillus plantarum and tilapia, applied in the field of feed, can solve the problems of alleviating lead poisoning of aquatic organisms, which have not been reported by anyone, and achieve the effect of alleviating oxidative stress damage, accelerating growth rate, and improving the activity of intestinal digestive enzymes

Inactive Publication Date: 2016-02-03
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Moreover, a large number of scholars have studied the probiotic effect of probiotic feed on aquatic animals, but no one has reported on alleviating lead poisoning in aquatic organisms.

Method used

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  • Plant lactobacillus feed capable of relieving tilapia lead toxicity
  • Plant lactobacillus feed capable of relieving tilapia lead toxicity
  • Plant lactobacillus feed capable of relieving tilapia lead toxicity

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Example 1 Effect of mixing on the activity of feed CGMCC NO.5494 in different ways of adding probiotics

[0051] The concentrated bacterial liquid, freeze-dried bacterial powder, and sodium alginate microcapsule bacterial agent produced by CGMCCNO.5494 were added to the basic feed of tilapia, and mixed into powdered feed by a mixer, and the powdered feed was counted by colony plate counting method. The content of live bacteria in the feed is detected, and the obtained result is multiplied by the weight of the feed, which is recorded as M; the colony plate count is carried out on the concentrated bacterial liquid, freeze-dried bacterial powder and sodium alginate microcapsule bacterial agent before adding to the feed, and the total number of live bacteria is calculated. , recorded as M0, the survival rate=M / M0×100% was obtained. The results are shown in Table 3:

[0052] Table 3 Effect of mixing on the survival rate of feed CGMCCNO.5494 with different probiotic addition...

Embodiment 2

[0056] Example 2 Effect of granulation on the activity of feed CGMCC NO.5494 in different ways of adding probiotics

[0057] The concentrated bacterial liquid, freeze-dried bacterial powder, and sodium alginate microcapsule bacterial agent produced by CGMCCNO.5494 were added to the basic feed of tilapia, and mixed into powdered feed by a mixer. Colony plate counting was used for detection; the mixed powder feed was granulated in a granulator at a granulation temperature of 70°C, and the live bacteria content in the pelleted feed was detected by colony plate counting after granulation. The results are shown in Table 4:

[0058] Table 4 Effect of granulation on the survival rate of feed CGMCC5494 with different probiotic addition methods

[0059]

[0060] Note: Different letters represent significant differences between groups (P<0.05)

[0061] The granulation unit operation had a great influence on the activity of CGMCCNO.5494 with different addition methods. The method o...

Embodiment 3

[0062] Example 3 Effect of storage on the activity of feed CGMCCNO.5494 in different ways of adding probiotics

[0063] The granulated feed processed with concentrated bacterial liquid, freeze-dried bacterial powder, sodium alginate microcapsules and sprayed after granulation was stored at 4°C and 25°C for two months, and the pelleted feed was plated once every other week. Count the number of live bacteria and calculate their survival rate.

[0064] The experimental results are attached figure 2 , attached image 3 shown, by attaching figure 2 It can be seen that under the condition of 4°C, the activity of CGMCCNO.5494 in each group showed good stability, as shown in the attached figure 2 As shown in (a), within one month, the activities of CGMCC NO.5494 in the four groups can all be maintained at the same order of magnitude. In the 2-month storage experiment, the sodium alginate microcapsule group showed the best stability, and the number of viable bacteria could still...

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Abstract

The invention discloses a plant lactobacillus feed capable of relieving tilapia lead toxicity, and belongs to the technical field of feed. The feed is a finished feed probiotics granulated feed having the viable bacterial number of more than 10<8> cfu / g and prepared by a way of adding a bacterial agent obtained by carrying out centrifuging concentration or freeze drying and microcapsule embedding on CGMCC 5494 strains into an aquatic animal feed pre-mix material in a form of a feed additive, and then granulating or pre-granulating and then spraying probiotics on a carrier to obtain the feed. The feed obtained by the method can increase the growth rate of tilapia, increases the intestinal tract digestive enzyme activity of the lead-exposure tilapia, reduces the lead content in bodies of the lead-exposure tilapia, relieves the oxidative stress damage in the bodies of the lead-exposure tilapia, improves the activity of aminolevtllinic acid dehydratase in the bodies of the lead-exposure tilapia, improves the lysozyme activity and myeloperoxidase activity of the lead-exposure tilapia, relieves the cell and genetic toxicity of the lead-exposure tilapia, and better has application prospects.

Description

technical field [0001] The invention relates to a Lactobacillus plantarum feed capable of alleviating the lead toxicity of tilapia, and belongs to the technical field of feed. Background technique [0002] Lead is a heavy metal element that is very common in nature and has strong toxicity to the human body. In 1993, the World Health Organization and the Food and Agriculture Organization of the United Nations (FAO / WHO) Joint Codex Committee recommended that the human body's tolerable lead intake was 25 μg / (kg·body weight·week). Metal lead is soft and is one of the earliest metals used by humans. Its main purpose is to make lead storage batteries, and it is widely used in gasoline antiknock agents, construction, metal processing materials, munitions, color TV picture tubes, and radiation shielding. Because lead-acid batteries provide energy storage, military and civilian production and life are inseparable from it. With the advancement of the economy and society, especially ...

Claims

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Application Information

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IPC IPC(8): A23K50/80A23K10/16A23K10/20A23K10/30
CPCY02A40/818
Inventor 陈卫田丰伟翟齐啸王汗成王刚赵建新刘小鸣张秋香张灏
Owner JIANGNAN UNIV
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