A method for constructing a mixed bacterial system and application thereof in degrading long-chain alkanes
By constructing Pseudomonas aeruginosa PAO1-2, which produces high levels of rhamnolipids, and Yersinia lipolytica Po1g-8, which efficiently degrades long-chain alkanes, and by heterologously expressing alkane monooxygenase LadA and endogenously expressing alkane transporter ABC1, we achieved efficient degradation of long-chain alkanes in an artificial mixed-culture system during fermentation. This solved the problem of low degradation rate in existing technologies and improved the degradation efficiency of long-chain alkanes in wastewater.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- TIANJIN UNIV OF SCI & TECH
- Filing Date
- 2026-04-09
- Publication Date
- 2026-07-17
AI Technical Summary
Existing technologies have low degradation rates for petroleum hydrocarbons, making it difficult to effectively remove long-chain alkane pollutants, especially in wastewater where degradation efficiency is insufficient.
We constructed a high-yielding Pseudomonas aeruginosa PAO1-2 and a highly efficient long-chain alkanes-degrading Yersinia lipolytica Po1g-8. Through heterologous expression of alkane monooxygenase LadA and endogenous expression of alkane transporter ABC1, we utilized an artificial mixed-culture system to synergistically degrade long-chain alkanes during fermentation.
It significantly improved the degradation rate of long-chain alkanes, with the mixed bacteria experimental group achieving a degradation rate of 88.15%, demonstrating a significant advantage in degrading long-chain alkanes in wastewater.
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