Culture method of flammulina velutipes liquid strains

Abstract

The invention discloses a culture method of flammulina velutipes liquid strains, which comprises the steps of the culture medium preparation, sterilization, inoculated culture and the like. A culture medium comprises soybean powder, white granulated sugar, magnesium sulfate and monopotassium phosphate; and after the liquid culture medium is added into a liquid strain culture tank with an external heat source, the culture tank is pushed into a sterilizing pot for sterilization, the culture tank is cooled to room temperature for inoculation after the sterilization ends, and then sterile compressed air is introduced into the culture tank for culture. The culture medium of the method has the advantages of reasonable formula and rich nutrients; the liquid culture medium in the liquid strain culture tank adopting the external heat source is heated uniformly without damaging nutrient components. The liquid strains cultured by the method have the advantages of short production cycle, easy quality control, high spawn running speed, tidy fruiting, and high yield.

Description

technical field [0001] The invention belongs to the technical field of edible fungus cultivation, in particular to a method for cultivating liquid strains of Flammulina velutipes. Background technique [0002] In the prior art, solid strains are generally used in the production of Flammulina velutipes, and the production method of the solid strains is: inoculating the original seed of Flammulina velutipes on a solid medium rich in lignocellulose or starch and culturing it. The use of solid strains has the following disadvantages: (1) the production cycle is long, the production cycle of solid strains needs 26 days, and the cultivation of solid strains takes 31 days; Testing cannot ensure that the strains used are excellent, and cannot prevent the occurrence of pollution; (3) The solid strains have inconsistent bacterial growth speeds and bacterial ages, resulting in differences in production yields; (4) Solid strains develop bacteria The speed is slow, and it generally take...

AI Technical Summary

Problems solved by technology

The use of solid strains has the following disadvantages: (1) the production cycle is long, the production cycle of solid strains needs 26 days, and the cultivation of solid strains takes 31 days; Testing cannot ensure that the strains used are excellent, and cannot prevent the occurrence of pollution; (3) The solid strains have inconsistent bacterial growth speeds and bacterial ages, resulting in differences in production yields; (4) Solid strains develop bacteria The speed is slow, it usually takes 7 days to cover the surface of the material, and the probability of pollution is high

[0003] In recent years, people began to carry out the research of liquid strain fermentation and culture technology, and all have remarkable achievements in the breeding of strain, the inoculation method of liquid strain, the formula of culture medium and special equipment, but for the liquid strain of Flammulina velutipes Cultivation is still blank