Culture method of flammulina velutipes liquid strains

A technology of liquid strains and culture methods, which is applied in the direction of botany equipment and methods, fungi, fertilizer mixtures, etc., can solve the problems of inability to eliminate pollution, long production cycle, and undetectable, etc., and achieve easy control of the quality of strains, and bacteria The effect of short production cycle and fast germination speed

Active Publication Date: 2010-07-14
SHANGHAI XUERONG BIOTECH
View PDF0 Cites 17 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The use of solid strains has the following disadvantages: (1) the production cycle is long, the production cycle of solid strains needs 26 days, and the cultivation of solid strains takes 31 days; Testing cannot ensure that the strains used are excellent, and cannot prevent the occurrence of pollution; (3) The solid strains have inconsistent bacterial growth speeds and bacterial ages, resulting in differences in production yields; (4) Solid strains develop bacteria

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] Embodiment 1: a kind of cultivation method of Flammulina velutipes liquid bacterial classification, it comprises the steps:

[0012] (1) Culture medium preparation: Dissolve 1750g of bean curd powder in water after ultrafine grinding, dissolve 1750g of white sugar in hot water, dissolve 420g of magnesium sulfate and 420g of potassium dihydrogen phosphate in water, and add them to the liquid strain of external heat source respectively In the culture tank, the height of the tank is 1386mm, the diameter is 779mm, and the water is fixed to 700 liters;

[0013] (2) Sterilization: After adding the liquid culture medium into the liquid strain culture tank with an external heat source, push the culture tank into the sterilizer for sterilization, maintain the temperature at 100°C for 60 minutes, and maintain the temperature at 121°C for 60 minutes;

[0014] (3) Inoculation culture: After the sterilization is completed, cool to room temperature for inoculation, and then inoculate...

Embodiment 2

[0015] Embodiment 2: a kind of culture method of Flammulina velutipes liquid bacterial classification, it comprises the steps:

[0016] (1) Medium preparation: Dissolve 2000g of bean curd powder in water after ultrafine grinding, dissolve 1500g of white sugar in hot water, dissolve 400g of magnesium sulfate and 500g of potassium dihydrogen phosphate in water, and add them to the liquid strain of external heat source respectively In the culture tank, the height of the tank is 1386mm, the diameter is 779mm, and the water is fixed to 700 liters;

[0017] (2) Sterilization: After adding the liquid culture medium into the liquid strain culture tank with an external heat source, push the culture tank into the sterilizer for sterilization, maintain the temperature at 98°C for 70 minutes, and maintain the temperature at 120°C for 70 minutes;

[0018] (3) Inoculation culture: After the sterilization is completed, cool to room temperature for inoculation, and then inoculate with sterile...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
Heightaaaaaaaaaa
Diameteraaaaaaaaaa
Login to view more

Abstract

The invention discloses a culture method of flammulina velutipes liquid strains, which comprises the steps of the culture medium preparation, sterilization, inoculated culture and the like. A culture medium comprises soybean powder, white granulated sugar, magnesium sulfate and monopotassium phosphate; and after the liquid culture medium is added into a liquid strain culture tank with an external heat source, the culture tank is pushed into a sterilizing pot for sterilization, the culture tank is cooled to room temperature for inoculation after the sterilization ends, and then sterile compressed air is introduced into the culture tank for culture. The culture medium of the method has the advantages of reasonable formula and rich nutrients; the liquid culture medium in the liquid strain culture tank adopting the external heat source is heated uniformly without damaging nutrient components. The liquid strains cultured by the method have the advantages of short production cycle, easy quality control, high spawn running speed, tidy fruiting, and high yield.

Description

technical field [0001] The invention belongs to the technical field of edible fungus cultivation, in particular to a method for cultivating liquid strains of Flammulina velutipes. Background technique [0002] In the prior art, solid strains are generally used in the production of Flammulina velutipes, and the production method of the solid strains is: inoculating the original seed of Flammulina velutipes on a solid medium rich in lignocellulose or starch and culturing it. The use of solid strains has the following disadvantages: (1) the production cycle is long, the production cycle of solid strains needs 26 days, and the cultivation of solid strains takes 31 days; Testing cannot ensure that the strains used are excellent, and cannot prevent the occurrence of pollution; (3) The solid strains have inconsistent bacterial growth speeds and bacterial ages, resulting in differences in production yields; (4) Solid strains develop bacteria The speed is slow, and it generally take...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A01G1/04C05G1/00C12N1/14
Inventor 余荣琳
Owner SHANGHAI XUERONG BIOTECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap