Method for preparing flammulina velutipes polysaccharides and protein efficiently synchronously

A technology of Flammulina velutipes polysaccharide and Flammulina velutipes, which is applied in the fields of peptide preparation, chemical instruments and methods, organic chemistry, etc., can solve the problems of inability to prepare protein components at the same time, waste, low extraction rate, etc., achieves less explosion, realizes regeneration and recovery , the effect of simple operation

Inactive Publication Date: 2013-05-22
JIAXING VOCATIONAL TECHN COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the defects that the extraction rate is low when extracting Flammulina velutipes polysaccharides in the prior art and protein components cannot be prepared at the same time

Method used

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  • Method for preparing flammulina velutipes polysaccharides and protein efficiently synchronously

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Embodiment 1 (method 1 for simultaneously and efficiently preparing polysaccharide and protein from Flammulina velutipes)

[0034] (1) Raw material pretreatment: After crushing 10g of dried Flammulina velutipes fruiting bodies, mix it with distilled water at a weight ratio of 1:30 to form a mixture, and set aside;

[0035] (2) Enzymolysis treatment: Add 0.05% of cellulase by weight to the mixture, adjust the pH value to 4, and place it on a shaker at 45°C for 30 minutes;

[0036] (3) Microwave-assisted extraction: Step (2) Microwave treatment of the enzymatic hydrolyzate (80H Z , 500W) after 5 minutes, centrifuge at 6000 rpm for 10 minutes to obtain supernatant and sediment;

[0037] (4) Alcohol-precipitation of polysaccharides. Concentrate the supernatant of step (3) under reduced pressure with a rotary evaporator to one-fifth of the original volume. Centrifuge for 10 minutes, freeze and dry to obtain 0.92g of Flammulina velutipes crude polysaccharide;

[0038] (5...

Embodiment 2

[0042] Embodiment 2 (from Flammulina velutipes simultaneously and efficiently prepare the method two of polysaccharide and albumen)

[0043](1) Raw material pretreatment: After crushing 10 g of dried Flammulina velutipes fruiting bodies, mix it with distilled water at a weight ratio of 1:40 to form a mixture, and set aside;

[0044] (2) Enzymolysis treatment: Add 0.1% cellulase by weight of the mixture to the mixture, adjust the pH value to 4.5, place it on a shaker at 48°C for 50 minutes;

[0045] (3) Microwave-assisted extraction: Step (2) Microwave treatment of the enzymatic hydrolyzate (80H Z , 800W) after 10 minutes, centrifuge at 6000 rpm for 10 minutes to obtain supernatant and sediment;

[0046] (4) Alcohol-precipitation of polysaccharides. Concentrate the supernatant of step (3) under reduced pressure with a rotary evaporator to one-fifth of the original volume. Centrifuge for 10 minutes, freeze and dry to obtain 1.04g of Flammulina velutipes crude polysaccharide;...

Embodiment 3

[0051] Embodiment 3 (from Flammulina velutipes the method three of preparing polysaccharide and albumen simultaneously)

[0052] (1) Raw material pretreatment: After crushing 10g of dried Flammulina velutipes fruiting bodies, mix it with distilled water at a weight ratio of 1:45 to form a mixture, and set aside;

[0053] (2) Enzymolysis treatment: Add 0.15% of the weight of cellulase to the mixture, adjust the pH value to 5.5, and place it on a shaker at 50°C for 70 minutes;

[0054] (3) Microwave-assisted extraction: Step (2) Microwave treatment of the enzymatic hydrolyzate (50H Z , 500W) after 15 minutes, centrifuge at 6000 rpm for 10 minutes to obtain supernatant and sediment;

[0055] (4) Alcohol-precipitation of polysaccharides. Concentrate the supernatant of step (3) under reduced pressure with a rotary evaporator to one-fifth of the original volume. Centrifuge for 10 minutes, freeze and dry to obtain 1.11 g of Flammulina velutipes crude polysaccharide;

[0056] (5)...

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Abstract

The invention discloses a method for preparing flammulina velutipes polysaccharides and protein efficiently synchronously. The method comprises the following steps of: grinding flammulina velutipes sporocarps, pretreating by using a cellulose, extracting by microwave, centrifuging and precipitating in alcohol to obtain the crude flammulina velutipes polysaccharides; dissolving the crude flammulina velutipes polysaccharides into water, adding an ionicliquid/salt aqueous two-phase system, mixing uniformly and centrifuging to obtain an upper phase and a lower phase; taking out the lower layer which is rich in the crude flammulina velutipes polysaccharides, removing salt by using an ultrafiltration membrane with the retention capacity of 6,000, concentrating, freezing and drying to obtain the flammulina velutipes polysaccharides; adding methylene dichloride into the upper layer which is rich in proteins, extracting, drying rotationally and removing the methylene dichloride to obtain the flammulina velutipes protein. The method can be used for preparing the flammulina velutipes polysaccharides and protein synchronously, can improve the extraction rate of the polysaccharides and the protein, is simple in process and low in equipment requirement and is suitable for large-scale production.

Description

technical field [0001] The invention belongs to the technical field of extraction and separation of effective components of edible fungi, and in particular relates to a method for simultaneously and efficiently preparing polysaccharides and proteins of Flammulina velutipes. Background technique [0002] Flammulina velutipes ( Flammulina velutipes ) has another name called mushroom, mushroom, belongs to the fungi door, Basidiomycetes, Agaricales, Tricholomaceae, Chrysomycetes. Flammulina velutipes is extremely nutritious, and has the laudatory titles such as Zengzhi mushroom and "Yixiu mushroom". It is a famous edible fungus in the world and has developed into the third largest edible fungus at present. Flammulina velutipes is tender and refreshing, delicious and fragrant, and rich in nutrition. It is a very high-end delicacy with high nutritional value. According to literature reports, Flammulina velutipes contains higher protein, carbohydrates and crude fiber, while the f...

Claims

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Application Information

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IPC IPC(8): C08B37/00C07K1/14
Inventor 贺伟强
Owner JIAXING VOCATIONAL TECHN COLLEGE
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