Fermented composition of multiple probiotics and composite medicinal and edible fungi, and preparation method and application thereof

A technology of medicinal and edible fungi and fermented composition, which is applied in the application field of health care products or medicines

Active Publication Date: 2013-10-23
SANZHU FUER PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Previous studies have shown that total polysaccharides of Angelica sinensis have a positive effect on solid tumor S 180 No obvious inhibitory effect; but low molecular weight Angelica polysaccharide fraction on S 180 The tumor inhibition rate of solid tumors can reach 36%

Method used

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  • Fermented composition of multiple probiotics and composite medicinal and edible fungi, and preparation method and application thereof
  • Fermented composition of multiple probiotics and composite medicinal and edible fungi, and preparation method and application thereof
  • Fermented composition of multiple probiotics and composite medicinal and edible fungi, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Embodiment 1: the preparation of the fermented composition of probiotics fermentation Ganoderma lucidum, Agaricus blazei, mushroom, Flammulina velutipes

[0034] Take dried Ganoderma lucidum, Agaricus blazei, shiitake mushrooms, and Flammulina velutipes fruit bodies, and use ordinary crushing technology to pulverize the above fungi for both medicinal and edible purposes. Take fungal powder: Ganoderma lucidum 4.0g, Agaricus blazei 30g, Lentinus edodes 40g, Flammulina velutipes 10g, add drinking water equivalent to 60 times the weight of the composite bacterial powder to the fungus powder, soak for 30 minutes, and heat at 100°C for 1 hour, When the temperature is cooled to about 40°C, add the compound enzyme according to the ratio of 3% (volume ml / weight g) of the enzyme to the compound bacterial powder (cellulase: pectinase: protease = 1:2:1, Taian Xindeli Bioengineering Co., Ltd.), after 3 hours of enzymatic hydrolysis at 37°C~50°C, boil for 10 minutes to inactivate the...

Embodiment 2

[0035] Embodiment 2: Preparation of the fermented composition of probiotic fermented Agaricus blazei, shiitake mushroom, and Flammulina velutipes composite fungal powder

[0036] Take dried Agaricus blazei, shiitake mushrooms, and Flammulina velutipes fruiting bodies, crush them, and the bacteria powder is required to pass through a 65-mesh sieve. Take fungal bacteria powder: Agaricus blazei 20 g, shiitake mushrooms 20 g, Flammulina velutipes 10 g, add 1000ml of drinking water to soak for 1 hour, 100 Heat at ℃ for 1 hour, and when the temperature cools down to about 40℃, add compound enzyme according to the ratio of 3% (volume ml / weight g) of enzyme to compound bacterial powder (cellulase: pectinase: protease = 1:2: 1, produced by Taian Xindeli Bioengineering Co., Ltd.), after 3 hours of enzymatic hydrolysis at 37 ° C ~ 50 ° C, boil for 10 minutes to inactivate the enzyme, and obtain the enzymatic hydrolysis complex polysaccharide extract with residue left. Then add glucose 0....

Embodiment 3

[0037] Example 3: Changes in polysaccharide molecular weight distribution between fermented and unfermented compositions of Agaricus blazei, shiitake mushrooms, and Flammulina velutipes fungal powder compound polysaccharide fermented by intestinal probiotics

[0038] The sample prepared in Example 2 was selected, and the composition without probiotic fermentation was used as a control.

[0039] The purpose of the test is to examine the polysaccharide molecular weight distribution of the compound medicinal and edible fungus after the fermentation and transformation of probiotics. In the polysaccharide extraction experiment, alcohol precipitation is the first step in the separation and purification of polysaccharides, and alcohol precipitation is separated and purified according to the molecular weight. When the concentration of alcohol precipitation is small, the polysaccharides that can be precipitated are often polysaccharides with larger molecular weights. The concentration...

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Abstract

The invention discloses a fermented composition of multiple probiotics and composite medicinal and edible fungi, and a preparation method and an application thereof. According to the invention, a composite polysaccharide extract is obtained by enzymolysis and extraction of various medicinal and edible fungi by using compound enzymes, and the extract is subjected to symbiotic fermentation by using multiple intestinal probiotics, such that a fermented composition is obtained. The invention also relates to an application of the fermented composition in preparing health-care products used for preventing and treating tumors. The medicinal and edible fungi adopted by the invention are any three or more than three selected from lucid ganoderma, agaricus blazei murill, shiitake, and flammulina velutipes. The fungi are subjected to crushing, soaking, and enzymolysis; and bifidobacterium and lactobacillus are added for fermentation. Through symbiotic fermentation with the intestinal probiotics, the molecular weight of medicinal and edible fungi composite polysaccharide is substantially reduced, tumor cell inhibiting activity is substantially improved, and inhibition effect is substantially improved.

Description

technical field [0001] The invention relates to the technical field of biological fermentation, in particular to three or more of Ganoderma lucidum, Agaricus blazei, shiitake mushrooms, and Flammulina velutipes, which are compound polysaccharide extracts of medicinal and edible fungi that are extracted by enzymolysis with compound enzymes. The fermented composition prepared after symbiotic fermentation, and the application of the fermented composition in the preparation of health products or medicines for preventing and treating tumors. Background technique [0002] The fungus with both medicine and food has anti-cancer activity. In 1968, Japanese scholar Qianyuan Wulang first used hot water to separate substances that can inhibit tumors from the fruiting bodies of shiitake mushrooms, and analyzed and determined them. It was confirmed that these substances are mainly Some high molecular polysaccharides. Since then, scientists from all over the world have conducted a lot of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/074A61K31/715A61P37/04A61P35/00A61K35/74A61K35/745A61K35/747
Inventor 吴炳新孙筱林褚新红曹向英王静
Owner SANZHU FUER PHARMA
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