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Application of Hydrogen Sulfide in Improving the Inhibition Rate of Antitumor Drugs

A technology of hydrogen sulfide and sulfide, applied in the field of medicine, can solve the problems of large side effects, etc., and achieve the effects of small side effects, reduced damage, and reduced dosage

Inactive Publication Date: 2018-06-22
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] Therefore, it is necessary to explore a new anticancer drug synergist to solve the problem of large side effects of the above anticancer drugs

Method used

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  • Application of Hydrogen Sulfide in Improving the Inhibition Rate of Antitumor Drugs
  • Application of Hydrogen Sulfide in Improving the Inhibition Rate of Antitumor Drugs
  • Application of Hydrogen Sulfide in Improving the Inhibition Rate of Antitumor Drugs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1～4

[0036] Embodiments 1 to 4 are processed according to the following method (methylazotetrazolium salt (MTT) analysis method):

[0037] Methylazotetrazole solution: 250 mg of methylazotetrazole salt was dissolved in 50 mL of phosphate buffer (PBS, 0.01 mol / L, pH 7.4), filtered and sterilized, and stored in a refrigerator at 4°C.

[0038] Other materials: DMEM medium containing 10% fetal bovine serum, 0.25% trypsin, etc.

[0039] Methylazotetrazolium salt (MTT) analysis method: After 0.25% trypsin digests monolayer cultured cells, prepare single cell suspension with DMEM culture medium containing 10% fetal bovine serum, count and dilute to 10000 cells / mL. In a 96-well culture plate, add 100 μL of single cell suspension to each well, transfer into CO 2 In an incubator, 37°C, 5% CO 2and cultured at saturated humidity for 72 hours. Then, carefully aspirate the culture medium and add 100 μL HO 2 S release the reagent, treat at room temperature for 10 minutes, carefully absorb t...

Embodiment 1

[0042] Example 1 Synergistic inhibition of growth of human lung cancer cell line A549 by potassium hydrosulfide and hydrogen peroxide

[0043] The in vitro growth characteristics of human lung cancer cell line A549 were tested by methylazotetrazolium salt (MTT) assay. It was found that potassium hydrosulfide treatment alone had no inhibitory effect on the growth of cancer cells, while hydrogen peroxide treatment alone had an inhibitory effect on cancer cells, and this inhibition was dose-related; Enhance the inhibitory effect on cancer cells, the synergistic inhibitory effect of the two is obvious (such as figure 1 shown).

Embodiment 2

[0044] Example 2 Synergistic inhibition of growth of human gastric cancer cell line SGC7901 by sodium sulfide and doxorubicin

[0045] The in vitro growth characteristics of the human gastric cancer cell line SGC7901 were tested using the methylazotetrazolium (MTT) assay. It was found that sodium sulfide treatment alone had no inhibitory effect on the growth of cancer cells, but doxorubicin alone had an inhibitory effect on cancer cells, and this inhibitory effect was dose-related; and adding doxorubicin after sodium sulfide treatment could significantly enhance the growth of cancer cells. The inhibitory effect on cancer cells, the synergistic inhibitory effect of the two is obvious (such as figure 2 shown).

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PUM

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Abstract

The invention discloses an application of hydrogen sulfide to increasing the tumor inhibition rate of an anti-tumor drug and an application of the hydrogen sulfide to preparing an anti-tumor drug synergist, and further discloses a method for increasing the tumor inhibition rate of the anti-tumor drug. The method includes the step that the anti-tumor drug synergist is applied to cancer cells before the anti-tumor drug is treated or while the anti-tumor drug is treated. According to the synergist, the hydrogen sulfide and soluble sulfur-hydrogen salts or soluble sulfide are used as effective ingredients. After the synergist acts on organism tumor cells, the tumor suppression effect of the anti-tumor drug can be improved obviously. Side effects on normal organism cells are quite small and even can be ignored, and the side effects on the normal cells are greatly reduced.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to the application of hydrogen sulfide in improving the tumor inhibition rate of antitumor drugs and a method for increasing the tumor inhibition rate of antitumor drugs. Background technique [0002] For tumor / cancer patients, there are no more than three treatment methods: surgery, radiotherapy and chemotherapy. The scope of application of surgery is relatively narrow, and it is generally only suitable for patients with benign tumors, and only unnecessary organs can be removed; radiotherapy and chemotherapy are the preferred options for most tumor / cancer patients. [0003] Radiation therapy is a method of killing cancer cells by using the radiation energy of radiation (such as X-rays, gamma rays, and electron rays). High doses of radiation can directly act on DNA, causing breakage or cross-linking of DNA molecules; low doses of radiation can cause tissue fluid ionization, result...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K33/04A61P35/00
Inventor 吴根福
Owner ZHEJIANG UNIV
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