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Method for cultivating pleurotus eryngii liquid strains

A technology for liquid strains and king oyster mushrooms is applied in the fields of botanical equipment and methods, horticulture, and application, which can solve the problems of cumbersome operation and waste of raw materials, and achieve the effects of simple preparation process, easy operation and shortening of production cycle.

Inactive Publication Date: 2014-01-15
SHANGHAI ACAD OF AGRI SCI +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Other liquid strain methods have been reported before, such as potatoes as a carbon source and bran as a nitrogen source, but this method is relatively cumbersome to operate in the actual factory production process and consumes raw materials

Method used

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  • Method for cultivating pleurotus eryngii liquid strains
  • Method for cultivating pleurotus eryngii liquid strains
  • Method for cultivating pleurotus eryngii liquid strains

Examples

Experimental program
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Embodiment 1

[0021] 1. Materials and methods:

[0022] The tested strains, the parent species of Pleurotus eryngii Quel. is Guosen No.1 from Shanghai Guosen Biotechnology Co., Ltd.

[0023] The mother seed shake flask medium includes: sugar 20g / L, soybean meal powder 3g / L, MgSO 4 0.6g / L, KH 2 PO 4 0.6 / L, the rest is water. And put in a magnetic stirrer rotor, the specification is 35mm in length and 8mm in width. The volume of the 1L graduated triangle bottle is 400ml. After sterilizing, insert 3 to 5 pieces of 1 cm 2 test tube slant original seeds, and put them into a shaker at 21 °C with a rotation speed of 120 rpm for 8 days.

[0024] The composition of the culture medium in the fermenter is shown in Table 1.

[0025] The partial formula composition of table 1 culture fluid

[0026]

[0027]

[0028] ① Put the sugar into the container, add 10L of water, stir to dissolve;

[0029] ②, add MgSO 4 and KH 2 PO 4 and dissolve it;

[0030] ③. Filter the bean paste powder throu...

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Abstract

The invention discloses a method for cultivating pleurotus eryngii liquid strains. The method comprises the steps that firstly, liquid mother culture is prepared, then the liquid strains are cultured through fermentation cylinders, and finally, the obtained liquid strains through preparation can be injected into cultivation bottles or cultivation bags for cultivation through an injecting machine.

Description

technical field [0001] The invention belongs to the technical field of industrial cultivation of edible fungi, and in particular relates to a method for cultivating liquid strains of Pleurotus eryngii. Background technique [0002] Compared with solid strains, liquid strains of Pleurotus eryngii have the advantages of short production cycle, consistent bacterial age, complete mushroom production, easy management, low cost of strains, and convenient and rapid inoculation. And it is also conducive to the scale and factory production of edible fungi. [0003] At present, the production method of Pleurotus eryngii in China is mainly the cultivation of solid strains, which has a long production cycle, irregular fruiting, and high energy consumption. To develop the Pleurotus eryngii cultivation industry, the application of liquid strains and corresponding cultivation techniques is an ideal shortcut. Other liquid strain methods have been reported before, such as potatoes as a car...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/04
Inventor 鲍大鹏王涛李正鹏李玉周峰谭琦
Owner SHANGHAI ACAD OF AGRI SCI
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