Microorganism fodder production method based on pleurotus eryngii mushroom cultivating residues

A technology of microbial feed and Pleurotus eryngii mushroom residue, which is applied in animal feed, animal feed, application, etc., can solve the problems of ineffective degradation of lignocellulose, decreased efficiency of cellulose degradation, and decreased activity of digestive enzymes, and achieves improved Food digestibility and biological potency, regulation of normal flora, effect of reducing barriers

Inactive Publication Date: 2012-10-10
牛管家文化产业(北京)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But in this patent, without adding cellulase, the efficiency of degrading cellulose is greatly reduced, because Bacillus subtilis, yeast, lactic acid bacteria, etc. cannot effectively degrade lignocellulose
Moreover, the probiotics in this patent have undergone one-step fermentation,

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] A kind of microbial feed production method based on Pleurotus eryngii fungus dregs, when carrying out the first production, at first carry out one-stage cellulase pretreatment and aerobic probiotic fermentation, specific method is as follows:

[0030] (1) Bacillus culture medium

[0031]Bacillus slant and plate culture medium: Use 10g of peptone, 5g of beef extract powder, 5g of sodium chloride, 15g of agar, 20g of glucose, and 1000ml of distilled water. After mixing, adjust the pH to 7.0±0.2. Sterilize at 121°C for 20 minutes.

[0032] Bacillus shake flask seed medium and seed tank medium: beef extract 5.0g / L, peptone 20.0g / L, glucose 5.0g / L, FeCl2 6H2O 0.07g / L, MnC12 7H2O 0.01g / L, MgSO4 7H2O 0.15g / L, adjust pH = 7.0, and sterilize at 121°C for 20min.

[0033] (2) Yeast medium

[0034] Yeast slant medium and plate culture medium (100 ml): use 2 g of glucose, 1 g of yeast extract, 2 g of peptone, and 2 g of agar, with a pH value of about 6.0.

[0035] Shake flask se...

Embodiment 2

[0082] A kind of microbial feed production method based on Pleurotus eryngii fungi dregs is characterized in that comprising the following steps successively:

[0083] First, prepare the first-stage fermentation medium: crush the Pleurotus eryngii sticks in a wet state; then add cellulase, ammonium salt, urea, calcium salt, potassium salt and magnesium salt, and mix well; the first-stage fermentation medium The ammonium salt is at least one of ammonium nitrate, ammonium sulfate and ammonium chloride; the calcium salt is at least one of calcium nitrate and calcium chloride; the magnesium salt is at least one of magnesium sulfate, magnesium nitrate and magnesium chloride The weight content of each component is respectively: cellulase 2000 units / Kg; Ammonium salt 0.15%; Urea 0.15%; Calcium salt 2%; Potassium dihydrogen phosphate 1%; Magnesium salt 1%; % of Pleurotus eryngii sticks powder.

[0084] Secondly, yeast and bacillus are inoculated in the first-stage fermentation medium...

Embodiment 3

[0088] A kind of microbial feed production method based on Pleurotus eryngii fungi dregs is characterized in that comprising the following steps successively:

[0089] First, prepare the first-stage fermentation medium: crush the Pleurotus eryngii sticks in a wet state; then add cellulase, ammonium salt, urea, calcium salt, potassium salt and magnesium salt, and mix well; the first-stage fermentation medium The weight content of each component is respectively: ammonium salt is ammonium nitrate 0.08%, ammonium sulfate 0.05%, ammonium chloride 1%; calcium salt is calcium nitrate 2.5%, calcium chloride 2%; magnesium salt is magnesium sulfate 0.05% , magnesium nitrate 0.05%; cellulase 2000 units / Kg; urea 1%; potassium dihydrogen phosphate 1%; water 50%;

[0090] Secondly, yeast and bacillus were inoculated in the first-stage fermentation medium, and the first-stage aerobic fermentation was carried out; the yeast and bacillus used for inoculation were the first-stage fermentation m...

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PUM

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Abstract

The invention discloses a microorganism fodder production method based on pleurotus eryngii mushroom cultivating residues and belongs to the technical field of fodder production. The microorganism fodder production method comprises the following steps of: firstly, preparing a first-stage fermentation culture medium based on the pleurotus eryngii mushroom cultivating residues; then inoculating saccharomycetes and bacillus in the first-stage fermentation culture medium to carry out first-stage aerobic fermentation; then, adding soya bean meals and rapeseed meals to prepare a second-stage fermentation culture medium; and finally, inoculating lactobacillus and bifidobacterium into the second-stage fermentation culture medium and uniformly mixing; and carrying out anaerobic fermentation to obtain a finished product. When the fodder is used for feeding animals, the fodder conversion rate is low, the investment is less and the output is high; and meanwhile, wastes in the production of pleurotus eryngii are effectively utilized. According to the microorganism fodder obtained by the invention, raw materials do not need to be sterilized and products do not need to be subjected to drying treatment; the obtained biological fodder is enriched with active probiotic bacteria, functional enzymes and auxiliary factors, and the probiotic bacteria and the functional enzymes are remained; and the fodder is low in price and excellent in performance and the adding amount can reach 5-40% in a feeding process; and therefore, economic benefits and social meanings are good.

Description

technical field [0001] The invention belongs to the technical field of feed, in particular to a microbial feed or a microbial feed additive. Background technique [0002] At present, the market scale of the feed industry is huge, and it is increasing at a rate of about 15% per year, but the profit is very low. Most of them are produced with grain as raw material, and the cost is difficult to reduce. It will be one of the future development directions to produce cheap and high-quality biological feed through a series of microbial treatments from non-grain raw materials. At present, there are only a small number of domestic companies engaged in the research and development and production of fermented feed or biological feed, but they also use grain as the main raw material, so it is difficult to greatly reduce the cost, and it is not easy to achieve great development and high profits. Some companies use anaerobic fermentation as the main process of biological feed, and use no...

Claims

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Application Information

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IPC IPC(8): A23K1/00A23K1/16A23K1/175
Inventor 陈华友杨胜利谢永明
Owner 牛管家文化产业(北京)有限公司
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