Microorganism fodder production method based on pleurotus eryngii mushroom cultivating residues
A technology of microbial feed and Pleurotus eryngii mushroom residue, which is applied in animal feed, animal feed, application, etc., can solve the problems of ineffective degradation of lignocellulose, decreased efficiency of cellulose degradation, and decreased activity of digestive enzymes, and achieves improved Food digestibility and biological potency, regulation of normal flora, effect of reducing barriers
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Embodiment 1
[0029] A kind of microbial feed production method based on Pleurotus eryngii fungus dregs, when carrying out the first production, at first carry out one-stage cellulase pretreatment and aerobic probiotic fermentation, specific method is as follows:
[0030] (1) Bacillus culture medium
[0031]Bacillus slant and plate culture medium: Use 10g of peptone, 5g of beef extract powder, 5g of sodium chloride, 15g of agar, 20g of glucose, and 1000ml of distilled water. After mixing, adjust the pH to 7.0±0.2. Sterilize at 121°C for 20 minutes.
[0032] Bacillus shake flask seed medium and seed tank medium: beef extract 5.0g / L, peptone 20.0g / L, glucose 5.0g / L, FeCl2 6H2O 0.07g / L, MnC12 7H2O 0.01g / L, MgSO4 7H2O 0.15g / L, adjust pH = 7.0, and sterilize at 121°C for 20min.
[0033] (2) Yeast medium
[0034] Yeast slant medium and plate culture medium (100 ml): use 2 g of glucose, 1 g of yeast extract, 2 g of peptone, and 2 g of agar, with a pH value of about 6.0.
[0035] Shake flask se...
Embodiment 2
[0082] A kind of microbial feed production method based on Pleurotus eryngii fungi dregs is characterized in that comprising the following steps successively:
[0083] First, prepare the first-stage fermentation medium: crush the Pleurotus eryngii sticks in a wet state; then add cellulase, ammonium salt, urea, calcium salt, potassium salt and magnesium salt, and mix well; the first-stage fermentation medium The ammonium salt is at least one of ammonium nitrate, ammonium sulfate and ammonium chloride; the calcium salt is at least one of calcium nitrate and calcium chloride; the magnesium salt is at least one of magnesium sulfate, magnesium nitrate and magnesium chloride The weight content of each component is respectively: cellulase 2000 units / Kg; Ammonium salt 0.15%; Urea 0.15%; Calcium salt 2%; Potassium dihydrogen phosphate 1%; Magnesium salt 1%; % of Pleurotus eryngii sticks powder.
[0084] Secondly, yeast and bacillus are inoculated in the first-stage fermentation medium...
Embodiment 3
[0088] A kind of microbial feed production method based on Pleurotus eryngii fungi dregs is characterized in that comprising the following steps successively:
[0089] First, prepare the first-stage fermentation medium: crush the Pleurotus eryngii sticks in a wet state; then add cellulase, ammonium salt, urea, calcium salt, potassium salt and magnesium salt, and mix well; the first-stage fermentation medium The weight content of each component is respectively: ammonium salt is ammonium nitrate 0.08%, ammonium sulfate 0.05%, ammonium chloride 1%; calcium salt is calcium nitrate 2.5%, calcium chloride 2%; magnesium salt is magnesium sulfate 0.05% , magnesium nitrate 0.05%; cellulase 2000 units / Kg; urea 1%; potassium dihydrogen phosphate 1%; water 50%;
[0090] Secondly, yeast and bacillus were inoculated in the first-stage fermentation medium, and the first-stage aerobic fermentation was carried out; the yeast and bacillus used for inoculation were the first-stage fermentation m...
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