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Agrobacterium with heterotrophic nitrification-aerobic denitrification capability and application thereof in nitrogenous effluent treatment

A technology of aerobic denitrification and Agrobacterium, applied in biological water/sewage treatment, water/sludge/sewage treatment, bacteria, etc., has broad application prospects, saves equipment and investment costs, and simplifies the process flow

Inactive Publication Date: 2009-11-04
LANGFANG GAIA ENVIRONMENTAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] The purpose of the present invention is to overcome the problem that anoxic denitrification and aerobic nitrification must be treated in stages in the existing biological denitrification process, and to provide a highly efficient heterotrophic nitrification-aerobic denitrification with natural growth advantages. Bacterial strains - Agrobacterium sp., and its application in the treatment of nitrogenous wastewater

Method used

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  • Agrobacterium with heterotrophic nitrification-aerobic denitrification capability and application thereof in nitrogenous effluent treatment
  • Agrobacterium with heterotrophic nitrification-aerobic denitrification capability and application thereof in nitrogenous effluent treatment
  • Agrobacterium with heterotrophic nitrification-aerobic denitrification capability and application thereof in nitrogenous effluent treatment

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Embodiment 1

[0034] Agrobacterium sp. strain LAD9 with heterotrophic nitrification-aerobic denitrification performance is characterized in that: the 16S rRNA gene of the Agrobacterium strain has a nucleotide sequence as shown in the sequence table, and the sequence length is 1418bp, The accession number in Genbank is FJ639330, and its deposit number is CGMCC No.2962.

Embodiment 2

[0036] As above-mentioned Agrobacterium sp. (Agrobacterium sp.) bacterial strain LAD9 is characterized in that: this Agrobacterium is a strain of leather obtained through domestication, isolation and purification from sampling in the immobilized aerated biological filter tank for processing landfill leachate. Lambert-negative bacteria.

Embodiment 3

[0038] As above-mentioned Agrobacterium sp. (Agrobacterium sp.) bacterial strain LAD9 is characterized in that: at 30 ℃, after cultivating on the nutrient agar medium for 2 days, the bacterial colony is raised, the entire margin is smooth, and there is no pigment; after Gram staining It was negative under the microscope, and the bacteria were rod-shaped, with a size of (1.5-0.3) μm×(0.6-1.0) μm, arranged singly and in pairs, and did not form spores.

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Abstract

The invention relates to an agrobacterium strain (Agrobacterium sp.) with heterotrophic nitrification-aerobic denitrification capability and an application thereof in nitrogenous effluent treatment. The bacterial strain is characterized in that: 16S rRNA gene contains nucleotide sequence shown in sequence table with a sequence length of 1418bp and a accession number FJ639330 in Genbank. The preservation number thereof is CGMCC No.2962. The agrobacterium of the invention has not only heterotrophic nitrification capability but also aerobic denitrification capability. During nitrogenous effluent treatment, and one aerobic phase can cause ammonian to change into gaseous product with high denitrification efficiency and easy operation and tremendous economic benefit compared with the traditional biological denitrification process.

Description

technical field [0001] The invention relates to an Agrobacterium strain, in particular to an Agrobacterium capable of converting ammonia nitrogen in waste water into gas products under aerobic conditions and its application in nitrogenous waste water treatment. Background technique [0002] The main forms of nitrogen in wastewater are molecular nitrogen, organic nitrogen, ammonia nitrogen, nitrite nitrogen, nitrate nitrogen, and some nitrogen present in hydrogen sulfide and cyanide. In untreated raw wastewater, organic nitrogen and ammonia nitrogen are the main forms of nitrogen. Due to the toxic effect of ammonia nitrogen, the carcinogenic effect of nitrate, and the acidification and eutrophication of nitrogen compounds on ecosystems, rivers, lakes, and offshore oceans, how to economically and efficiently remove nitrogen in water has become a water pollution treatment technology. Research priorities and hotspots in the field of control. [0003] Among the various denitrif...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/01C02F101/16C02F101/38
Inventor 陈倩倪晋仁
Owner LANGFANG GAIA ENVIRONMENTAL TECH
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