Pseudomonas aeruginosa being tolerant to zinc ion toxicity and application thereof

A Pseudomonas aeruginosa, zinc ion technology, applied in bacteria, microorganism-based methods, biological water/sewage treatment, etc., can solve problems affecting the quality of effluent from urban sewage treatment systems, and achieve huge economic and environmental benefits. Efficient removal, cost savings in equipment and investment

Active Publication Date: 2017-09-15
PEKING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Studies have shown that the concentration of Zn(II) exceeding 10 mg / L will affect the N in traditional denitrification. 2 The reduction process of O, denitrification rate and denitrification microbial activity are significantly inhibited, and at the same time, the microbial community structure is changed, thereby affecting the effluent quality of urban sewage treatment systems

Method used

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  • Pseudomonas aeruginosa being tolerant to zinc ion toxicity and application thereof
  • Pseudomonas aeruginosa being tolerant to zinc ion toxicity and application thereof
  • Pseudomonas aeruginosa being tolerant to zinc ion toxicity and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1. Utilize scanning electron microscope to observe the apparent morphology of Pseudomonas aeruginosa in the presence of zinc ions

[0025] The high-efficiency aerobic denitrification strain used in this experiment is Pseudomonas aeruginosa, which was isolated from the leachate treatment system of the landfill site.

[0026] Inoculate Pseudomonas aeruginosa into 1L of LB medium (containing 5g of NaCl, 10g of tryptone, and 5g of yeast extract per liter) to prevent the invasion of miscellaneous bacteria and maintain the growth vigor of the bacteria for enrichment nourish. Centrifuge the cultured bacterial liquid, wash three times with 0.5% NaCl, and make the optical density OD 600 For 1-2 bacterial suspensions. Then inoculate the bacterial suspension in the LB medium containing 0, 10, 50, 100mg / L Zn(II) according to the inoculum amount of 10%, and take samples after 8h and centrifuge at 8000rpm and 4°C for 5min to remove the supernatant solution and washed twice...

Embodiment 2

[0028] Example 2. The aerobic denitrification characteristics of Pseudomonas aeruginosa in the presence of 10mg / L Zn(II)

[0029] The denitrification performance test medium (BM) formula is per liter of water: 8.45g CH 3 COONa, 0.63g NH 4 Cl, 0.61g NaNO 3 ,1.76g K 2 HPO 4 ·3H 2 O, 0.20g MgSO 4 ·7H 2 O, 0.02g CaCl 2 ,0.005g FeSO 4 ·7H 2 O, 0.1mL trace element solution. Adjust the pH value of the medium to 7.5, and sterilize at 121°C for 30min.

[0030] Pseudomonas aeruginosa (Pseudomonas aeruginosa) was inoculated in BM medium containing 10mg / L Zn(II), and cultured with shaking at 30°C and 150rpm, and 100μL headspace was extracted every 5h with a valve injection needle body for the determination of N 2 O, use a sterile syringe to draw 2mL of gas and inject it into a 2L pure helium bag for the determination of NO. At the same time, 2 mL of the bacterial suspension was extracted with a sterile syringe, and the bacterial liquid was centrifuged at 8000 rpm for 5 min at...

Embodiment 3

[0032] Example 3. The aerobic denitrification characteristics of Pseudomonas aeruginosa in the presence of 50mg / L Zn(II)

[0033] Pseudomonas aeruginosa was inoculated in BM medium containing 50mg / L Zn(II) to test its aerobic denitrification performance. The result is as image 3As shown, 100mg / L nitrate nitrogen was utilized by the strain immediately after inoculation, and was completely consumed within 15h, and the average removal rate of nitrate nitrogen was 6.67mg / L / h. With the reduction of nitrate nitrogen, nitrite nitrogen gradually accumulated and reached the highest value of 35.5mg / L at 10h, and then was reduced at 20h. N 2 O also accumulated gradually, reaching the highest value of 84.4mg / L at 20h, and then completely reduced at 45h. It must be pointed out that at this time, the highest NO accumulation in the intermediate product of the denitrification process is 3.2μg / L, accounting for only 0.0032% of the nitrate nitrogen removal. It can be seen that the strain s...

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Abstract

The invention relates to a strain of pseudomonas aeruginosa being tolerant to zinc ion toxicity and an application thereof, and provides a pseudomonas aeruginosa strain which has zinc ion tolerance and is assigned the accession number of CGMCC No.14063. When the concentration of zinc ion in wastewater is 0-100 mg/L, the bacterial strain can survive with nitrate nitrogen as only nitrogen source, and under an aerobiotic environment, removal rate on the nitrate nitrogen can reach 100% and removal rate on total nitrogen can reach 100% too. The pseudomonas aeruginosa strain has convenience in operation and is high in denitrification efficiency. The bacterial strain has important significance of optimizing a wastewater bio-treatment system and reducing the influence of zinc ions on the wastewater denitrification system.

Description

technical field [0001] The invention belongs to the field of sewage biological treatment, in particular to a Pseudomonas aeruginosa which can withstand the toxicity of zinc ions and can simultaneously remove nitrate nitrogen in water under aerobic conditions and its application in sewage treatment. Background technique [0002] With the acceleration of urbanization and industrialization in our country and the continuous development of industrial production, a large amount of industrial wastewater is produced, which poses a great threat to human life and health. Since my country's industrial wastewater treatment capacity cannot keep up with the scale of industrial water use, some industrial wastewater is still discharged directly into the urban sewage pipe network without treatment. As an important industrial material, zinc ions are widely used in chemical industry, metallurgy, electroplating, tanning and coating industries. These industries will produce a large amount of zin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C02F3/02C12R1/385C02F101/16
CPCC02F3/02C02F3/34C02F2101/163C12N1/205C12R2001/385Y02W10/10
Inventor 倪晋仁桂梦瑶郑茂盛
Owner PEKING UNIV
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