A colorless bacterium resistant to nickel ion toxicity and its application

A colorless bacillus, nickel ion technology, applied in the direction of bacteria, microorganism-based methods, biological water/sewage treatment, etc., can solve the problems of microbial toxicity, achieve efficient removal, strong tolerance to nickel ion toxicity and aerobic denitrification The effect of capacity, cost savings on equipment and investment

Active Publication Date: 2021-07-23
PEKING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Trace amounts of Ni(II) can promote the growth of microorganisms, but Ni(II) also has strong acute biological toxicity, and when it exceeds a certain concentration, it will have a toxic effect on microorganisms

Method used

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  • A colorless bacterium resistant to nickel ion toxicity and its application
  • A colorless bacterium resistant to nickel ion toxicity and its application
  • A colorless bacterium resistant to nickel ion toxicity and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1. Aerobic denitrification characteristics of Achromobacter in the presence of 0mg / L Ni(II)

[0026] The denitrification performance test medium (BM) formula is per liter of water: 8.45g CH 3 COONa, 0.63g NH 4 Cl, 0.61g NaNO 3 ,1.76g K 2 HPO 4 ·3H 2 O, 0.20g MgSO 4 ·7H 2 O, 0.02g CaCl 2 ,0.005g FeSO 4 ·7H 2 O, 0.1mL trace element solution. Adjust the pH value of the medium to 7.5, and sterilize at 121°C for 30min.

[0027] Achromobacter sp. was inoculated in BM medium, shaken at 30°C and 150 rpm, and 100 μL of headspace gas was extracted every 5 hours with a valve needle for the determination of N 2 O, 2 mL of gas was extracted with a sterile syringe and injected into a 2 L pure helium bag for the determination of NO. At the same time, 2 mL of bacterial suspension was extracted with a sterile syringe, and the bacterial liquid was centrifuged at 8000 rpm for 5 min at 4°C, and the supernatant was taken for analysis of ammonia nitrogen, nitrate nitrogen...

Embodiment 2

[0029] Embodiment 2. Aerobic denitrification characteristics of Achromobacter in the presence of 2mg / L Ni(II)

[0030] The denitrification performance test medium (BM) formula is per liter of water: 8.45g CH 3 COONa, 0.63g NH 4 Cl, 0.61g NaNO 3 ,1.76g K 2 HPO 4 ·3H 2 O, 0.20g MgSO 4 ·7H 2 O, 0.02g CaCl 2 ,0.005g FeSO 4 ·7H 2 O, 0.1mL trace element solution. Adjust the pH value of the medium to 7.5, and sterilize at 121°C for 30min.

[0031] Achromobacter sp. was inoculated in BM medium containing 2mg / L Ni(II), shaken at 30°C and 150rpm, and 100μL of headspace gas was extracted every 5h with a valve injection needle. for the determination of N 2 O, 2 mL of gas was extracted with a sterile syringe and injected into a 2 L pure helium bag for the determination of NO. At the same time, 2 mL of bacterial suspension was extracted with a sterile syringe, and the bacterial liquid was centrifuged at 8000 rpm for 5 min at 4°C, and the supernatant was taken for analysis of am...

Embodiment 3

[0033] Example 3. Aerobic denitrification characteristics of Achromobacter in the presence of 5mg / L Ni(II)

[0034] Achromobacter sp. was inoculated in BM medium containing 5 mg / L Ni(II) to test its aerobic denitrification performance. The result is as image 3 As shown, 100 mg / L nitrate nitrogen was utilized by the strain immediately after inoculation and was completely consumed within 20 h, with an average removal rate of 5.0 mg / L / h nitrate nitrogen. With the reduction of nitrate nitrogen, nitrite nitrogen gradually accumulated and reached the highest value of 32.2mg / L at 15h, and then was reduced at 30h. N 2 O also accumulated gradually, reaching the highest value of 2.63mg / L at 20h, and then completely reduced at 30h. It must be pointed out that the highest NO accumulation in the intermediate product of the denitrification process is 4.2 μg / L, accounting for only 0.004% of the nitrate nitrogen removal. It can be seen that the strain still has the ability of efficient aer...

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Abstract

The present invention relates to a strain of Achromobacter sp. resistant to nickel ion toxicity and its application. The present invention provides a strain of Achromobacter sp. with nickel ion tolerance, whose deposit number is CGMCC No.2964. When the nickel ion concentration in the sewage is in the range of 0-10mg/L, the strain can use nitrate nitrogen as the only nitrogen source. Under aerobic conditions, the removal rate of nitrate nitrogen can reach 100%, and the removal rate of total nitrogen is also up to 100%. The invention has the advantages of convenient operation and high denitrification efficiency, and is of great significance for optimizing the sewage biological treatment system and reducing the influence of nickel ions on the sewage denitrification system.

Description

technical field [0001] The invention belongs to the field of sewage biological treatment, and in particular relates to an achromobacter which can withstand the toxicity of nickel ions and simultaneously remove nitrate nitrogen in water under aerobic conditions and its application in sewage treatment. Background technique [0002] With the acceleration of urbanization and industrialization in our country and the continuous development of industrial production, a large amount of industrial wastewater is produced, which poses a great threat to human life and health. Since my country's industrial wastewater treatment capacity cannot keep up with the scale of industrial water use, some industrial wastewater is still discharged directly into the urban sewage pipe network without treatment. As an important industrial material, nickel ions are widely used in chemical industry, metallurgy, electroplating, tanning and coating industries. These industries will produce a large amount of...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/025C02F101/16
CPCC02F3/34C02F2101/163C12N1/205C12R2001/025
Inventor 陈倩孙卫玲王婷倪晋仁
Owner PEKING UNIV
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