Achromobacter strain with hexavalent chromium removal and aerobic denitrification performance and its application

An aerobic denitrification and achromobacter technology, applied in aerobic process treatment, bacteria, chemical instruments and methods, etc., can solve the problems of microbial death, affecting the growth of microorganisms, affecting the normal operation of urban sewage treatment systems, etc., to simplify The effect of process flow, cost saving, huge economic and environmental benefits

Active Publication Date: 2020-09-11
PEKING UNIV
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Studies have shown that hexavalent chromium will affect the growth of most microorganisms, and even cause a large number of microorganisms to die, and urban sewage treatment plants usually do not have the ability to deal with hexavalent chromium, so the entry of hexavalent chromium will seriously affect the urban sewage treatment system. normal operation

Method used

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  • Achromobacter strain with hexavalent chromium removal and aerobic denitrification performance and its application
  • Achromobacter strain with hexavalent chromium removal and aerobic denitrification performance and its application
  • Achromobacter strain with hexavalent chromium removal and aerobic denitrification performance and its application

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Embodiment 1. Synchronous chromium and denitrification characteristics of achromobacter in the presence of 2mg / L Cr(VI)

[0025] The denitrification performance test medium (BM) formula is per liter of water: 8.45g CH 3 COONa, 0.63g NH 4 Cl, 0.61g NaNO 3 ,1.76g K 2 HPO 4 ·3H 2 O, 0.20g MgSO 4 ·7H 2 O, 0.02g CaCl 2 ,0.005g FeSO 4 ·7H 2 O, 0.1mL trace element solution. Adjust the pH value of the medium to 7.5, and sterilize at 121°C for 30min.

[0026] Achromobacter sp. was inoculated in BM medium containing 2 mg / L Cr(VI), shaken at 30°C and 150 rpm, and 100 μL of headspace gas was extracted every 5 hours with a valve injection needle. for the determination of N 2 O, 2 mL of gas was extracted with a sterile syringe and injected into a 2 L pure helium bag for the determination of NO. At the same time, 2 mL of bacterial suspension was extracted with a sterile syringe, and the bacterial liquid was centrifuged at 8000 rpm for 5 min at 4 °C, and the supernatant wa...

Embodiment 2

[0028] Embodiment 2. The synchronous chromium and denitrification characteristics of Achromobacter in the presence of 4mg / L Cr(VI)

[0029] Achromobacter sp. was inoculated in BM medium containing 4mg / L Cr(VI) to test its aerobic denitrification performance. The result is as figure 2 As shown, 100 mg / L nitrate nitrogen was utilized by the strain immediately after inoculation and was completely consumed within 20 h, with an average removal rate of 5.0 mg / L / h nitrate nitrogen. With the reduction of nitrate nitrogen, nitrite nitrogen gradually accumulated and reached the highest value of 53.5mg / L at 15h, and then was reduced at 25h. N 2 O also accumulated gradually, reaching the highest value of 10.2mg / L at 25h, and then completely reduced at 40h. It must be pointed out that the highest NO accumulation in the intermediate product of the denitrification process is 9.5 μg / L, accounting for only 0.0095% of the nitrate nitrogen removal; at the same time, the removal rate of Cr(VI...

Embodiment 3

[0030] Embodiment 3. Synchronous chromium and denitrification characteristics of Achromobacter in the presence of 8mg / L Cr(VI)

[0031] Achromobacter sp. was inoculated in BM medium containing 8mg / L Cr(VI) to test its aerobic denitrification performance. The result is as image 3 As shown, 100 mg / L nitrate nitrogen was utilized by the strain immediately after inoculation and was completely consumed within 30 h, and the average removal rate of nitrate nitrogen was 3.33 mg / L / h. With the reduction of nitrate nitrogen, nitrite nitrogen gradually accumulated and reached the highest value of 64.2mg / L at 20h, and then was reduced at 40h. At the same time, N 2O also accumulated gradually, reaching the highest value of 21.6mg / L at 35h, and then completely reduced at 50h. It must be pointed out that the highest NO accumulation in the intermediate product of the denitrification process at this time is 7.6 μg / L, accounting for only 0.0076% of the nitrate nitrogen removal. At the same ...

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Abstract

The invention relates to a strain of Achromobacter sp. which has both hexavalent chromium removal and aerobic denitrification performances and its application in sewage treatment. The preservation number of the achromobacter is CGMCC № 2964, which not only has the ability to remove hexavalent chromium, but also has the ability to denitrify under aerobic conditions. When the concentration of hexavalent chromium in sewage is in the range of 4-8mg/L, the strain can use nitrate nitrogen as the only nitrogen source, and the removal rate of nitrate nitrogen can reach 100% in an aerobic environment, and the removal rate of hexavalent chromium Up to 70% or more. The invention has the advantages of convenient operation and high denitrification efficiency, and is of great significance for optimizing sewage biological treatment systems and reducing the influence of hexavalent chromium on sewage denitrification systems.

Description

technical field [0001] The invention belongs to the field of sewage treatment, and in particular relates to an achromobacter capable of synchronously removing nitrate nitrogen and hexavalent chromium in water under aerobic conditions and its application in sewage treatment. Background technique [0002] With the acceleration of urbanization and industrialization in our country and the continuous development of industrial production, a large amount of industrial wastewater is produced, which poses a great threat to human life and health. Since my country's industrial wastewater treatment capacity cannot keep up with the scale of industrial water use, some industrial wastewater is still discharged directly into the urban sewage pipe network without treatment. As an important industrial material, hexavalent chromium is widely used in electroplating, dye manufacturing, leather and metallurgy industries. These industries will generate a large amount of chromium-containing wastewa...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/02C02F3/34C12R1/025C02F101/22C02F101/16
CPCC02F3/02C02F3/34C02F2101/163C02F2101/22C12N1/205C12R2001/025Y02W10/10
Inventor 倪晋仁桂梦瑶陈倩
Owner PEKING UNIV
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