Pseudomonas stutzeri with both hexavalent chromium removal and aerobic denitrification performances and application of pseudomonas stutzeri

A technology of Pseudomonas stutzeri, aerobic denitrification, applied in bacteria, chemical instruments and methods, microorganism-based methods, etc., can solve the problems of microbial death, affecting the growth of microorganisms, affecting the normal operation of urban sewage treatment systems, etc. , to achieve the effect of simplifying the process flow, enhancing the practicability, efficiently removing hexavalent chromium and aerobic denitrification capacity

Active Publication Date: 2017-10-10
PEKING UNIV
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  • Application Information

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Problems solved by technology

Studies have shown that hexavalent chromium will affect the growth of most microorganisms, and even cause a large number of microorganisms to die, and urban sewage treatment plants usually do not have the ability to deal with hexavalent chromium, so the entry of hexavalent chromium will seriously affect the urban sewage treatment system. normal operation

Method used

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  • Pseudomonas stutzeri with both hexavalent chromium removal and aerobic denitrification performances and application of pseudomonas stutzeri
  • Pseudomonas stutzeri with both hexavalent chromium removal and aerobic denitrification performances and application of pseudomonas stutzeri
  • Pseudomonas stutzeri with both hexavalent chromium removal and aerobic denitrification performances and application of pseudomonas stutzeri

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Embodiment 1. Synchronous chromium and denitrification characteristics of Pseudomonas stutzeri in the presence of 2mg / L Cr(VI)

[0025] The denitrification performance test medium (BM) formula is per liter of water: 8.45g CH 3 COONa, 0.63g NH 4 Cl, 0.61g NaNO 3 ,1.76g K 2 HPO 4 ·3H 2 O, 0.20g MgSO 4 ·7H 2 O, 0.02g CaCl 2 ,0.005g FeSO 4 ·7H 2 O, 0.1mL trace element solution. Adjust the pH value of the medium to 7.5, and sterilize at 121°C for 30min.

[0026] Pseudomonas stutzeri (Pseudomonas stutzeri) was inoculated in BM medium containing 2 mg / L Cr(VI), shaken at 30°C and 150 rpm, and 100 μL of the top was extracted every 5 h with a valve injection needle. Air is used to measure N 2 O, 2 mL of gas was extracted with a sterile syringe and injected into a 2 L pure helium bag for the determination of NO. At the same time, extract 2 mL of bacterial suspension with a sterile syringe, centrifuge the bacterial solution at 8000 rpm for 5 min at 4°C, and take the su...

Embodiment 2

[0028] Embodiment 2. Synchronous chromium and denitrification characteristics of Pseudomonas stutzeri in the presence of 5mg / L Cr(VI)

[0029] Pseudomonas stutzeri (Pseudomonas stutzeri) was inoculated in BM medium containing 5 mg / L Cr(VI), and its aerobic denitrification performance was tested. The result is as figure 2 As shown, 100 mg / L nitrate nitrogen was utilized by the strain immediately after inoculation and was completely consumed within 15 h, with an average removal rate of 6.67 mg / L / h nitrate nitrogen. With the reduction of nitrate nitrogen, nitrite nitrogen gradually accumulated and reached the highest value of 49.2mg / L at 10h, and then was reduced at 20h. N 2 O also accumulated gradually, reaching the highest value of 4.8mg / L at 20h, and then completely reduced at 35h. It must be pointed out that the highest NO accumulation in the intermediate product of the denitrification process at this time is 5.3 μg / L, accounting for only 0.0053% of the nitrate nitrogen r...

Embodiment 3

[0030] Embodiment 3. Synchronous chromium and denitrification characteristics of Pseudomonas stutzeri in the presence of 10mg / L Cr(VI)

[0031] Pseudomonas stutzeri (Pseudomonas stutzeri) was inoculated in BM medium containing 10mg / L Cr(VI) to test its aerobic denitrification performance. The result is as image 3As shown, 100mg / L nitrate nitrogen was utilized by the strain immediately after inoculation, and was completely consumed within 20h, and the average removal rate of nitrate nitrogen was 5.0mg / L / h. With the reduction of nitrate nitrogen, nitrite nitrogen gradually accumulated and reached the highest value of 54.2mg / L at 15h, and then was reduced at 25h. At the same time, N 2 O also accumulated gradually, reaching the highest value of 12.3mg / L at 25h, and then completely reduced at 40h. It must be pointed out that at this time, the highest NO accumulation in the intermediate product of the denitrification process is 9.3μg / L, accounting for only 0.0093% of the nitrate...

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Abstract

The invention relates to pseudomonas stutzeri with both hexavalent chromium removal and aerobic denitrification performances and an application of the pseudomonas stutzeri in sewage treatment. The preservation number of the pseudomonas stutzeri is CGMCC (China general microbiological culture collection center) No. 14062, and the pseudomonas stutzeri has both capabilities of hexavalent chromium removal and denitrification in the aerobic condition. When hexavalent in sewage is within 0-10mg/L in concentration, a strain can take nitrate nitrogen as the sole nitrogen source, the removal rate of the nitrate nitrogen can reach 100% in the aerobic condition, and the removal rate of the hexavalent can reach 80% or even 90% above. The pseudomonas stutzeri and the application thereof have the advantages of convenience in operation, high denitrification efficiency and the like, and great significance is achieved in optimizing a sewage treatment system and weakening the effect of the hexavalent chromium on the sewage denitrification system.

Description

technical field [0001] The invention belongs to the field of sewage biological treatment, in particular to a Pseudomonas stutzeri which can simultaneously remove nitrate nitrogen and hexavalent chromium in water under an aerobic environment and its application in sewage treatment. Background technique [0002] With the acceleration of urbanization and industrialization in our country and the continuous development of industrial production, a large amount of industrial wastewater is produced, which poses a great threat to human life and health. Since my country's industrial wastewater treatment capacity cannot keep up with the scale of industrial water use, some industrial wastewater is still discharged directly into the urban sewage pipe network without treatment. As an important industrial material, hexavalent chromium is widely used in electroplating, dye manufacturing, leather and metallurgy industries. These industries will generate a large amount of chromium-containing ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/38C02F101/22
CPCC02F3/34C02F2101/22C12N1/205C12R2001/38
Inventor 倪晋仁桂梦瑶郑茂盛
Owner PEKING UNIV
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