Complex bacteria for preprocessing of restaurant garbage, preparation method and application thereof

A compound bacterial agent and bacillus technology, which can be applied to microorganism-based methods, biochemical equipment and methods, bacteria, etc., can solve problems such as spoilage and odor, proliferation of various microorganisms, and poor targeting.

Active Publication Date: 2009-02-04
BEIJING GOLDENWAY BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above-mentioned technologies all use the organic matter of the whole domestic waste as the substrate, and the purpose of biological treatment is wide. There are problems

Method used

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  • Complex bacteria for preprocessing of restaurant garbage, preparation method and application thereof
  • Complex bacteria for preprocessing of restaurant garbage, preparation method and application thereof
  • Complex bacteria for preprocessing of restaurant garbage, preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Bacillus stearothermophilus, Bacillus licheniformis, Zymomonas, Agrobacterium, Saccharomyces cerevisiae and Candida preserved at 4°C were respectively inoculated into ordinary broth liquid medium, the inoculation amount was 0.1% (v / v), Activation culture at 28-37°C for 8-18 hours; then transfer to common broth liquid medium, culture at 28-34°C for 8-18 hours, inoculum size 0.1-0.5% (v / v). After raising, each bacterial solution was mixed according to the following proportions: 15% of Bacillus stearothermophilus, 15% of Bacillus licheniformis, 12% of Zymomonas, 18% of Agrobacterium, 25% of Saccharomyces cerevisiae, 15% of Candida. Obtain the composite bacterial agent of embodiment 1.

Embodiment 2

[0021] Repeat the preparation steps of Example 1, after the activation culture, each bacterial solution is mixed in the following proportions: 20% of Bacillus stearothermophilus, 17% of Bacillus licheniformis, 13% of Zymomonas, 15% of Agrobacterium, 19% of Saccharomyces cerevisiae %, the ratio of Candida 16%, makes the composite bacterial agent of embodiment 2.

Embodiment 3

[0023] Repeat the preparation steps of Example 1, after the activation culture, each bacterial solution is mixed in the following proportions: 30% of Bacillus stearothermophilus, 15% of Bacillus licheniformis, 12% of Zymomonas, 13% of Agrobacterium, 20% of Saccharomyces cerevisiae %, the ratio of Candida 10%, the composite bacterial agent of embodiment 3 is made.

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Abstract

The present invention relates to a composite microbial agent, a preparation method thereof and an application in the preprocessing of kitchen garbage. The active ingredients of the composite microbial agent include 15 percent to 30 percent of bacillus stearothermophilus, 10 percent to 25 percent of bacillus licheniformis, 10 percent to 25 percent of zymomonas, 10 percent to 25 percent of agrobacterium, 15 percent to 30 percent of saccharomyces cerevisiae and 10 percent to 25 percent of candida, which are blended together. The composite microbial agent is prepared according to the following method: (1) the strains, which are preserved under the temperature of 4 DEG C, are respectively inoculated in ordinary broth media, the inoculation amount is 0.1 percent (v/v), and under the temperature between 28 DEG C and 37 DEG C, activating culture lasts for 8 to 18 hours; (2) the strains are then respectively transinoculated into ordinary broth media to be cultured for 8 to 18 hours under the temperature between 28 DEG C and 34 DEG C, the inoculation amount is between 0.1 percent (v/v) and 0.5 percent (v/v), and after culturing, microbial liquids are blended according to the proportion. The composite microbial agent can be used to preprocess kitchen garbage, inhibit the reproduction of pathogenic microorganisms in the kitchen garbage and reduce the deterioration and stink of the kitchen garbage, so that the kitchen garbage can be conveniently processed later, so the application prospect of the composite microbial agent is wide.

Description

technical field [0001] The invention relates to a composite bacterial agent and its preparation method and application, in particular to a composite bacterial agent, its preparation method and its application in the pretreatment of kitchen waste. Background technique [0002] Kitchen waste, commonly known as swill, is the waste and residues in food processing and table leftovers in the kitchen and catering industry. Most of the food waste is in a solid-liquid mixed state, and its chemical composition is complex, mainly including water, inorganic salts and various macromolecular organic substances (such as protein, fat, cellulose and starch). Food waste is rich in various nutrients and contains a variety of microorganisms. During storage and transportation, the activities of microorganisms can easily cause food waste to deteriorate. The more common bacteria are Shewanella putrefaciens, Alcaligenes, Serratia, Micrococcus, Achromobacter, Flavobacterium, Bacillus pumilus, Pseud...

Claims

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Application Information

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IPC IPC(8): C12N1/00C12N1/20C12N1/16B09B5/00C12R1/07C12R1/10C12R1/01C12R1/865C12R1/72
Inventor 黄谦李桂生张琳
Owner BEIJING GOLDENWAY BIO TECH
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