Application of Garcinone E in preparation of tumor cell autophagosome inducer

An autophagosome and tumor cell technology, which can be used in anti-tumor drugs, medical preparations containing active ingredients, organic active ingredients, etc., and can solve problems such as poor induction effect.

Inactive Publication Date: 2020-12-22
GUANGXI MEDICAL UNIVERSITY
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Problems solved by technology

For example, in the process of autophagy research in nasopharyngeal carcinoma and ovarian cancer cells, we tried to use EBSS, Rapa and CQ to induce autophagosomes for nasopharyngeal cancer cells HK1, HONE1 and ovarian cancer cells HeyA8 and SKOV3, and found that The induction effect of EBSS and Rapa is not good, only CQ treatment can observe the positive result of autophagosome increase

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  • Application of Garcinone E in preparation of tumor cell autophagosome inducer
  • Application of Garcinone E in preparation of tumor cell autophagosome inducer
  • Application of Garcinone E in preparation of tumor cell autophagosome inducer

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[0022] The present invention will be described in detail below in conjunction with the accompanying drawings and specific embodiments. Unless otherwise specified, the reagents, equipment and methods used in the present invention are conventionally purchased reagents, equipment and conventional methods in the technical field, and the specific examples are only used to illustrate the present invention and are not limited to the scope of the present invention.

[0023] 1. Materials

[0024] 1. Cell lines: Ovarian cancer cell lines HeyA8, SKOV3 and nasopharyngeal cancer cell lines HK1 and HONE1 are preserved in our laboratory.

[0025] 2. Reagents: Garcinone E and Garcinone C are extracted from mangosteen husks, using the method of tracking and separating the target compound for alcohol extraction, silica gel column chromatography and gel column chromatography, to find the fraction group of the target compound, and then using preparative liquid chromatography A large number of ta...

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Abstract

The invention discloses application of Garcinone E in preparation of a tumor cell autophagosome inducer. It is found for the first time that Garcinone can effectively induce increase of autophagosomesof nasopharyngeal carcinoma and ovarian carcinoma cells, induce increase of expression levels of autophagy marker proteins LC3A/B-I and LC3A/B-II in nasopharyngeal carcinoma cells, and reduce the expression level of p62. A negative control compound Garcinone C which has the same mother ring structure as Garcinone E and has an inhibition effect on proliferation activity of tumor cells does not induce the increase of the number of autophagosomes of the nasopharyngeal carcinoma and ovarian carcinoma cells, induces increase of the expression levels of autophagy related proteins Beclin-1, LC3A/B-II, Atg7 and other proteins of the ovarian cancinoma cells, and induces no increase of the expression quantity of LC3A/B-I. It is prompted that Garcinone E can play an important role in preparation ofa novel autophagosome inducer for antitumor research.

Description

technical field [0001] The invention belongs to the technical field of cell autophagy, and in particular relates to the application of Garcinone E in the preparation of tumor cell autophagosome inducers. Background technique [0002] Garcinone E (molecular formula C 28 h 32 o 6 , molecular weight 464.55008, CAS No. 112649-21-5) is a xanthone monomer compound extracted from the peel of mangosteen, and studies have shown that it has anti-tumor effects. At present, there are few related studies on Garcinone E in tumors, especially ovarian cancer, and there is no report on the induction of autophagosomes in tumor cells. Garcinone C (molecular formula C 23 h 26 o 7 , molecular weight 414.44834, CAS No. 76996-27-5) is also a xanthone monomer compound extracted from mangosteen peel, which has the same mother ring structure as Garcinone E, and studies have shown that it has a significant effect on the proliferation activity of some tumor cells Inhibitory effect, also has anti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/352A61P35/00
CPCA61K31/352A61P35/00
Inventor 刘夏冯世秀尹富强董彩华雷顺媚
Owner GUANGXI MEDICAL UNIVERSITY
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