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Application of sinomenine to preparation of medicament for treating human brain glioma

A technology of human glioma and sinomenine, which is applied in drug combinations, antineoplastic drugs, and pharmaceutical formulations, can solve the problems of not being able to significantly inhibit the division and proliferation of U87MG glioma cells, and achieve the effect of broadening the application of medicine

Inactive Publication Date: 2017-06-20
EXPERIMENTAL RES CENT CHINA ACAD OF CHINESE MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For example, Curicumin can inhibit the proliferation of C6 glioma cells, but it cannot significantly inhibit the division and proliferation of U87MG glioma cells lacking the wild-type PTEN gene
[0005] At present, there is no report on sinomenine in anti-human glioma

Method used

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  • Application of sinomenine to preparation of medicament for treating human brain glioma
  • Application of sinomenine to preparation of medicament for treating human brain glioma
  • Application of sinomenine to preparation of medicament for treating human brain glioma

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1. Taking human glioma U87MG and SF767 cell lines as target cells to carry out the experiment on the inhibitory effect of sinomenine hydrochloride on human glioma cell lines

[0028] The experimental method is as follows: human glioma cell lines U87MG and SF767 provided by the Cell Resource Center of the Chinese Academy of Medical Sciences were digested with trypsin, suspended in the culture medium containing 10% fetal bovine serum, and gently blown with a glass dropper Cell suspension was formed, and viable cells were counted with a cell counting plate under a microscope. The tumor cells were inoculated in a sterile 96-well culture plate, and 100 μl of tumor cell suspension was added to each well. Place the cells at 37°C in 5% CO by volume 2 After 24 hours in the incubator, discard the supernatant, and then add different concentrations of sinomenine hydrochloride solutions. At 37°C, 5% CO by volume 2 Incubate in the incubator for 24 hours, 48 ​​hours, and 72...

Embodiment 2

[0030] Example 2, sinomenine hydrochloride can induce the increase of autophagosomes

[0031] Experimental method: After the cells are treated, centrifuge at 2000 rpm for 10 minutes, remove the upper layer of medium, and wash with cold PBS for 3 times, keep the lower layer of cells after centrifugation, gently add 2.5% glutaraldehyde fixative, and fix for 24 1 hour, 2% osmic acid fixation for 2 hours, gradient dehydration: 50%, 70%, 90%, 100% pyruvate dehydration, 10 minutes each level, 60°C embedding for 24 hours to make the cells block, section, transmission electron microscope Observed.

[0032] For observations see figure 2 As shown, compared with the control group in picture A, multiple double-layer membranous structures, namely autophagosomes, were observed in the cytoplasm of human glioma U87MG and SF767 cells after being treated with 0.5mM sinomenine hydrochloride. C is a partial enlargement of picture B. The results showed that sinomenine hydrochloride could induc...

Embodiment 3

[0033] Example 3, sinomenine hydrochloride can increase the number of autophagic vesicles

[0034] Experimental method: monodansyl pentadiamide (MDC) staining, which can selectively aggregate in acidic vesicles such as autophagosomes, lysosomes and autolysosomes, when MDC is under excitation light, it turns blue Green or yellow-green granular structures can be observed with a fluorescence microscope, which is a common method for detecting autophagy. Cells were seeded in a confocal single-well culture dish, 5×10 4 1 cell / well, 3 duplicate wells, add 100 μmol / LMDC working solution, place again in a 37-degree incubator and incubate for 30 minutes, wash the cells twice with PBS, add 1ml of 4% paraformaldehyde to fix for 15 minutes, wash the cells twice with PBS, and machine detection.

[0035] For experimental results, see image 3 shown. The results showed that acidic vesicles were rarely seen in the cells of the control group, but the number of acidic vesicles in the cells t...

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Abstract

The invention provides application of sinomenine to preparation of a medicament for treating human brain glioma. As proved by experiments, the sinomenine has a function of restraining human-derived glioma U87MG and SF767 cell strains, and the function is improved along with the increase of dose and time. Moreover, the sinomenine can induce the increase of autophagosomes, increase the quantity of autophagic vesicles, prompt the expression increase of human glioma cells LC3B-II, induce autophagic death of human glioma cells, and restrain the growth of U87MG cell xenograft tumors. The sinomenine is a novel and effective glioma-resisting medicament with a great potential, and can be used for preparing a human brain glioma medicament; by adopting the sinomenine, a new way is created for medical treatment of human brain glioma, and the medical application of the sinomenine is expanded.

Description

technical field [0001] The present invention relates to a new application of sinomenine in the field of medicine, more specifically, relates to the application of a kind of sinomenine hydrochloride in the preparation of medicine for treating human brain glioma. Background technique [0002] Glioma is the most common intracranial tumor, accounting for about 45% to 50% of all intracranial tumors. Malignant tumors are more common in this type of tumor, and due to the invasive growth of the tumor, traditional surgical treatment It is difficult to completely remove it, and it is a major disease that seriously threatens human health. In recent years, the treatment of malignant tumors has made significant progress. After more than 40 years of hard work, the research on anticancer drugs has developed to a new stage, and its research field has far exceeded the traditional cytotoxic drugs targeting nucleic acids and their components drugs. People have been working hard to find cytot...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/485A61P35/00
CPCA61K31/485
Inventor 王丹巧李连达焦玥姜宇懋王志国李涛
Owner EXPERIMENTAL RES CENT CHINA ACAD OF CHINESE MEDICAL SCI
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