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Use of hydrochloride coculine for preparing medicine to treat glioma

A technology of sinomenine hydrochloride and glioma, applied in the field of medicine of sinomenine hydrochloride, can solve problems such as difficult to completely remove, and achieve the effect of broadening the application of medicine

Inactive Publication Date: 2007-07-25
INST OF CHEM CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Malignant tumors are more common in this type of tumor, and due to the invasive growth of the tumor, it is difficult to completely remove it with traditional surgical treatment

Method used

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  • Use of hydrochloride coculine for preparing medicine to treat glioma
  • Use of hydrochloride coculine for preparing medicine to treat glioma

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] Embodiment 1, the preparation of sinomenine hydrochloride single crystal

[0013] Weigh 10 g of commercially available sinomenine hydrochloride powder, add dropwise 120 ml of NaOH solution with a pH value of 11.10 to dissolve the sinomenine hydrochloride sample. After stirring evenly, filter to remove insoluble matter. An HCl solution with a pH value of 4.00 was added dropwise to the solution, so that the pH value of the solution was reduced to 5.30. After standing at 4°C, colorless and transparent sinomenine hydrochloride crystals precipitated after 48 hours. Filter the solution, and wash the crystals successively with hydrochloric acid solution with a pH value of 5.30 and distilled water. After cleaning, put it into a petri dish, and vacuum-dry it for 24 hours to obtain 1.52 g of sinomenine hydrochloride single crystal.

Embodiment 2

[0014] Example 2. Taking the murine glioma C6 cell line as the target cell to carry out the experiment of the inhibitory effect of sinomenine hydrochloride on the glioma cell line

[0015] The sinomenine hydrochloride single crystal obtained in Example 1 was prepared into 100 μM and 500 μM aqueous solutions with distilled water, and then the tumor inhibition cell experiment was carried out according to the following method.

[0016] The experimental method is as follows: the murine glioma C6 cells provided by the Shanghai Cell Bank were digested with trypsin, suspended in 650 μl of culture medium containing 5 wt% calf serum, and gently blown into a cell suspension with a glass dropper. Viable cells were counted with a cytometer under a microscope. The tumor cells were inoculated on a sterile 96-well culture plate, and 200 μl of tumor cell suspension was added to each well. Place the cells at 37°C in 5% CO by volume 2 After 8-10 hours of pre-attachment in the incubator, the s...

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Abstract

An application of tuduranine hydrochloride in preparing the medicines for treating cerebral glioma is disclosed.

Description

technical field [0001] The invention relates to a new application of sinomenine hydrochloride in the field of medicine, in particular to an application of sinomenine hydrochloride in the preparation of drugs for treating glioma. Background technique [0002] Sinomenine is an alkaloid purified from the herbal medicine S. sinensis, which has anti-inflammatory, analgesic and immune control effects. Its tablets and injections have become officially approved drugs by the state. Now it has been used in the treatment of rheumatoid arthritis, and achieved satisfactory results. [0003] Modern pharmacological studies have shown that sinomenine has a variety of pharmacological effects. At present, in addition to the effect of sinomenine hydrochloride on the treatment of rheumatoid arthritis, reports on other drug effects include: analgesic and anti-inflammatory effects (Chinese Journal of Hospital Pharmacy, 25 (3), 196-198), treatment of chronic Nephritis medicament (Chinese patent ...

Claims

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Application Information

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IPC IPC(8): A61K31/485A61P35/00
Inventor 张虹向俊锋张亚周管烨陈旭征唐亚林
Owner INST OF CHEM CHINESE ACAD OF SCI
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