Traditional Chinese medicine composition for resisting skin photoaging and preparation method and application of compound preparation of traditional Chinese medicine composition

Abstract

The invention discloses a traditional Chinese medicine composition for resisting skin photoaging and a preparation method and application of a compound preparation of the traditional Chinese medicine composition. The traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: 70-90 parts of cornu cervi degelatinatum, 70-90 parts of radix angelicae sinensis, 70-90 parts of radix asparagi, 50-60 parts of rhizoma atractylodis macrocephalae, 50-60 parts of radix ampelopsis, 50-60 parts of rhizoma chuanxiong, 50-60 parts of semen armeniacae amarae, 50-60 parts of cassia twig and 50-60 parts of rhizoma zingiberis. Three medicinal materials including asarum, rhizoma typhonii and radix angelicae which are forbidden in cosmetics are removed from ancient prescription antler powder, on the basis of drug effect and property and flavor tropism, antler degelatinatum is used for replacing antler, three traditional Chinese medicines including rhizoma zingiberis, angelica sinensis and cassia twig are added, tedious operations such as soaking for a hundred days and grinding on stones in the ancient prescription are changed, and medicinal material components are obtained through an extraction method and prepared into a smearing preparation. The composition has a good protective effect in a skin photoaging model of middle-aged and aged mice, and especially has outstanding performance in the aspects of relieving wrinkle formation and collagen fiber damage caused by photoaging, relieving skin inflammation response and the like.

Description

Technical Field

[0001] This invention relates to the field of traditional Chinese medicine, and in particular to a traditional Chinese medicine composition for combating skin photoaging, a traditional Chinese medicine compound preparation, and its preparation method and application. Background Technology

[0002] Photoaging of the skin is mainly caused by chronic inflammatory responses triggered by ultraviolet (UV) radiation from sunlight. Oxidative stress, extracellular matrix damage, and inflammatory reactions are all important factors leading to skin aging. Ultraviolet (UV) radiation mainly includes long-wave UVA, medium-wave UVB, and short-wave UVC. UVB can penetrate the skin's surface, causing erythema and sunburn. UVA has extremely high penetrability, directly invading the body and damaging elastic fibers, thus harming the skin. Therefore, UVA and UVB are the main factors causing photoaging. On the one hand, excessive UV radiation can lead to pigmentation, dryness, roughness, sagging, and fine lines, affecting appearance and aesthetics. On the other hand, UV radiation can damage the skin's tissue structure and physiological functions, and can also cause high expression of matrix metalloproteinases (MMPs). MMPs can degrade collagen and elastic fibers, thereby affecting the normal structure of collagen fibers, reducing collagen synthesis, and causing photoaging manifestations such as roughness, sagging, looseness, and wrinkles. Therefore, how to effectively inhibit the skin photoaging process induced by ultraviolet radiation has become a hot research topic.

[0003] Traditional Chinese medicine texts contain numerous prescriptions for delaying skin aging, such as "improving complexion," "preserving youthful appearance," and "making the face radiant." In the 217th volume of *Qianjin Yaofang* (Qing Dynasty Wenyuange Siku Quanshu edition), written by Sun Simiao during the Tang Dynasty, under the section "Prescriptions for Acupuncture and Rectal Diseases," specifically the ninth prescription for facial medicine, there is a record: "Deer antler powder can make a centenarian's face look like that of a young person, primarily for a radiant and fair complexion. The ingredients are: deer antler, one handful long; cow's milk, three liters; chuanxiong, asarum, asparagus, angelica, atractylodes, white atractylodes, white aconite, ghee, three liang each; apricot kernels, five to seven pieces. These are the eleven ingredients." First, soak the deer antler in water for one hundred days. Then, add it to the other medicines and cow's milk, and simmer over low heat until all the juice is extracted and the antler is extracted. Place the remaining medicine in a white silk bag. Do not apply makeup. At night, grind the deer antler on a stone with cow's milk, apply the juice to your face, and wash it off with the paste in the morning. If there is no milk, you can also grind it with urine. As a traditional Chinese medicine formula, Deer Antler Powder has the effects of warming the kidneys and invigorating yang, promoting blood circulation and nourishing the complexion.

[0004] However, according to the "Cosmetic Safety Technical Specifications" (2015 edition), Asarum, Aconitum carmichaelii, and Angelica dahurica are listed as prohibited ingredients. Therefore, how to remove these three herbs from the formula based on efficacy, properties, and meridian tropism to improve safety while maintaining anti-aging potential is a pressing issue. In the traditional Deer Antler Powder formula, deer antlers need to be soaked in water for 100 days before use, and then ground on a stone with cow's milk or by urinating, which is clearly impractical. Therefore, it is necessary to develop a safer and easier-to-prepare formula based on the ancient Chinese medicine Deer Antler Powder to maximize its anti-photoaging effects. Summary of the Invention

[0005] To address the above shortcomings, this invention provides a traditional Chinese medicine composition for combating skin photoaging, based on the ancient formula Deer Antler Powder, along with its preparation method and application. The traditional Chinese medicine compound preparation can reduce skin aging and inflammatory reactions caused by ultraviolet radiation and promote the repair of photodamaged skin.

[0006] To achieve the above objectives, the specific technical solution of the present invention is as follows:

[0007] Part One: This invention provides a traditional Chinese medicine composition for resisting skin photoaging, made from the following raw materials in parts by weight: 70-90 parts of deer antler powder, 70-90 parts of angelica, 70-90 parts of asparagus, 50-60 parts of atractylodes macrocephala, 50-60 parts of white peony root, 50-60 parts of chuanxiong rhizome, 50-60 parts of bitter almond, 50-60 parts of cinnamon twig, and 50-60 parts of dried ginger.

[0008] Based on the above technical solutions, this invention, building upon the traditional deer antler powder formula, adjusts the formula to enhance its safety and practicality based on efficacy, properties, and meridian tropism. Specifically, by adjusting the proportions of raw materials, the invention removes three herbs—Asarum heterotropoides, Aconitum carmichaelii, and Angelica dahurica—that are prohibited under the "Cosmetic Safety Technical Specifications" (2015 edition). Based on efficacy and properties, deer antler powder is used to replace deer antler, and three additional herbs—dried ginger, Angelica sinensis, and cinnamon twig—are added.

[0009] The Chinese Pharmacopoeia (2025 edition) records that deer antler is salty and warm in nature, and enters the kidney and liver meridians. It has the effects of warming kidney yang, strengthening tendons and bones, promoting blood circulation, and reducing swelling. It also records that deer antler powder is obtained by boiling ossified antler to remove the gelatinous substance, extracting the antler pieces, and drying them. It is salty, astringent, and warm in nature, and enters the liver and kidney meridians. It has the functions of warming the kidney and assisting yang, and astringing and stopping bleeding. Therefore, deer antler powder and deer antler have similar properties and meridian tropism, both having the function of warming the kidney and assisting yang. Ancient prescriptions mention "grinding deer antler on a stone with cow's milk," and compared to deer antler, deer antler powder is easier to pulverize and extract. Therefore, this invention uses deer antler powder instead of deer antler.

[0010] Chuanxiong (Ligusticum striatum) is pungent, warm, and non-toxic. The Chinese Pharmacopoeia records that it can be used to invigorate blood circulation, dispel wind, and relieve pain. It has certain therapeutic effects on symptoms such as headaches, irregular menstruation, and invigorating blood circulation, hence it is called a "blood-qi-regulating herb." Asparagus root is a traditional Chinese medicinal plant. The Chinese Pharmacopoeia records that it is sweet, bitter, and cold. It enters the lung and kidney meridians. It has the effects of nourishing yin and moistening dryness, clearing lung heat and generating fluids. Modern pharmacological research shows that asparagus root has significant effects in anti-tumor, antibacterial, antioxidant, and anti-aging aspects. Atractylodes macrocephala (Bai Zhu) is bitter and sweet, warm in nature, and enters the spleen and stomach meridians. It has the effects of strengthening the spleen and replenishing qi, drying dampness and promoting diuresis, stopping sweating, and calming the fetus. Atractylodes macrocephala is a traditional Chinese medicine, and its underground parts, such as rhizomes and bulbs, have good antioxidant activity. Ampelopsis japonica (Bai Lian) is a traditional Chinese medicine, bitter and slightly cold in nature. It enters the heart and stomach meridians. It has the effects of clearing heat and detoxifying, eliminating carbuncles and dissipating nodules, and promoting tissue regeneration. Bitter almond (Ku Xing Ren) is bitter and slightly warm. It enters the lung and large intestine meridians. It lowers qi, relieves cough and asthma, and moistens the intestines to promote bowel movements. Bitter almonds are rich in vitamin E and unsaturated fatty acids, possessing antioxidant and moisturizing properties, helping to slow down the process of photoaging of the skin, promote repair, and soothe skin discomfort.

[0011] The ingredients Asarum, Aconitum carmichaelii, and Angelica dahurica in the ancient formula Deer Antler Powder are listed as prohibited ingredients in the *Cosmetic Safety Technical Specifications* (2015 edition). Asarum is pungent and warm, entering the heart, lung, and kidney meridians. Its functions are to relieve exterior syndromes and dispel cold, dispel wind and relieve pain, open the nasal passages, warm the lungs and resolve phlegm. Aconitum carmichaelii is pungent, warm, and toxic, entering the stomach and liver meridians. Its functions are to dispel wind and phlegm, calm the nerves, detoxify and dissipate nodules, and relieve pain. Angelica dahurica is pungent and warm, entering the stomach, large intestine, and lung meridians. Its functions are to relieve exterior syndromes and dispel cold, dispel wind and relieve pain, open the nasal passages, dry dampness and stop leukorrhea, reduce swelling and drain pus. Therefore, the main functions of all three are to relieve exterior syndromes and dispel cold, dispel wind and relieve pain. The *Chinese Pharmacopoeia* (2025 edition) records that dried ginger is pungent and hot, entering the spleen, stomach, kidney, heart, and lung meridians. It warms the middle jiao and dispels cold, restores yang and unblocks the meridians, warms the lungs and resolves phlegm. Cinnamon twig is pungent, sweet, and warm. It enters the Heart, Lung, and Bladder meridians. It promotes sweating and relieves muscle tension, warms and unblocks the meridians, assists Yang Qi transformation, and calms and descends Qi. Angelica sinensis is sweet, pungent, and warm. It enters the Liver, Heart, and Spleen meridians. It nourishes and invigorates blood, regulates menstruation and relieves pain, and moistens the intestines to relieve constipation. All three ingredients are pungent and warm or pungent and hot in nature. Among them, dried ginger and cinnamon twig both have the effect of inducing sweating and dispelling cold, similar to Asarum, Aconitum carmichaelii, and Angelica dahurica. The blood-invigorating effect of Angelica sinensis is similar to that of Ligusticum chuanxiong. Therefore, this invention removes Asarum, Aconitum carmichaelii, and Angelica dahurica from the formula to meet the requirements of the "Cosmetic Safety Technical Specifications" (2015 edition). Dried ginger, cinnamon twig, and Angelica sinensis are added to supplement the efficacy.

[0012] As an example, the traditional Chinese medicine composition is made from the following raw materials in parts by weight: 80 parts of deer antler powder, 80 parts of angelica, 80 parts of asparagus, 50 parts of atractylodes macrocephala, 50 parts of white peony root, 50 parts of chuanxiong rhizome, 50 parts of bitter almond, 50 parts of cinnamon twig, and 50 parts of dried ginger.

[0013] In the second part, the present invention provides a traditional Chinese medicine compound preparation for anti-skin photoaging, comprising any one of the traditional Chinese medicine compositions described above.

[0014] In some embodiments of the present invention, the traditional Chinese medicine compound preparation is an alcohol extract of the raw materials in the specified weight parts, preferably obtained by extraction with anhydrous ethanol.

[0015] Part Three, this invention provides a method for preparing the aforementioned traditional Chinese medicine compound preparation for anti-skin photoaging, comprising the following steps:

[0016] S1. After crushing each raw material, extract it in anhydrous ethanol and collect the extract.

[0017] S2. The extract is successively vacuum concentrated and freeze-dried to obtain lyophilized alcohol extract powder.

[0018] Based on the above technical solutions, this invention changes the cumbersome and complex preparation methods of traditional prescriptions, such as soaking for 100 days and grinding with a stone. It uses an ethanol reflux method to extract the effective components of the medicinal materials, which are then freeze-dried and combined with ethanol and propylene glycol to form a topical preparation, making it more convenient to use. This invention can fully utilize the efficacy of traditional Chinese medicine, improves the convenience of operation and use, and expands its applicability.

[0019] In some embodiments of the present invention, the ratio of the raw material to the anhydrous ethanol is 1g:(6-15)mL, including but not limited to 1g:10mL;

[0020] The extraction steps are as follows: first, mix and soak at room temperature for 12-24 hours, then heat and reflux at 82℃-90℃ for 45-60 minutes; preferably, the heating and reflux extraction is performed 2-3 times; as an example, first, mix and soak thoroughly at room temperature for 12 hours, then heat and reflux at 85℃ for 45 minutes, and after cooling to room temperature, filter the mixture and retain the filtrate; according to the above method, the filter residue is heated and refluxed twice more with anhydrous ethanol.

[0021] Part Four, the present invention provides the application of the traditional Chinese medicine composition described in any one of the above, the traditional Chinese medicine compound preparation described therein, or the traditional Chinese medicine relapse preparation prepared by the preparation method described above in any one of the following A1-A2:

[0022] A1. To prepare cosmetics that combat skin photoaging;

[0023] A2. Anti-photoaging of the skin for non-therapeutic purposes.

[0024] Experiments have shown that the traditional Chinese medicine compound preparation provided by this invention has a good protective effect in a photoaging model of middle-aged and elderly mice, particularly in slowing down the increase in skin thickness caused by photoaging, reducing wrinkle formation and collagen fiber damage, lowering the oxidative stress level of photoaged skin, maintaining the integrity of the skin extracellular matrix, and reducing skin inflammation. Therefore, the traditional Chinese medicine compound preparation of this invention eliminates factors affecting safety, improves ease of operation, expands the scope of application, and has good efficacy.

[0025] Specifically, the anti-photoaging cosmetics mentioned above can be anti-photoaging cosmetics for middle-aged and elderly people.

[0026] In some embodiments of the present invention, the anti-photoaging of skin is manifested in any one of the following aspects 1)-7): 1) improving the characteristics of photoaging skin or alleviating photoaging damage; 2) slowing down the increase in skin thickness and wrinkle formation caused by photoaging; 3) improving the inflammatory cell infiltration state of photoaging skin; 4) improving the damage to collagen fibers in photoaging skin; 5) reducing oxidative stress damage to photoaged skin; 6) maintaining the integrity of the skin extracellular matrix; 7) reducing inflammation caused by photoaging skin.

[0027] Fifthly, this invention provides a cosmetic product comprising the traditional Chinese medicine composition described in any one of the above-mentioned methods, the traditional Chinese medicine compound preparation described above, or the traditional Chinese medicine compound preparation prepared by the above-mentioned method.

[0028] Preferably, the freeze-dried powder obtained by freeze-drying is used to make a topical preparation, which includes, but is not limited to, toner, serum, emulsion, cream, spray, and tincture.

[0029] Compared with the prior art, the advantages of the present invention are:

[0030] The present invention provides a traditional Chinese medicine composition, a traditional Chinese medicine compound preparation for anti-skin photoaging, and their preparation methods and applications. Based on the ancient formula of Lujiao San, aiming at the deficiencies in terms of traditional Chinese medicinal materials prohibited by the "Technical Specifications for Cosmetics Safety" (2015 Edition), as well as the complex preparation methods and inconvenient use, etc., the present invention removes three traditional Chinese medicines, namely Asarum sieboldii, Typhonium giganteum, and Angelica dahurica, and based on the nature, flavor, meridian tropism and medicinal effects, adds three traditional Chinese medicines, namely Dry Ginger, Angelica sinensis, and Cinnamomum cassia, optimizes the formula and adopts the preparation process of ethanol reflux extraction and freeze-drying to improve its safety and the quality of the extract, and tests its anti-photoaging ability. The traditional Chinese medicine compound preparation of the present invention shows good protective effects on the mouse skin photoaging model, especially in slowing down the increase in skin thickness caused by skin photoaging, reducing the formation of wrinkles and the damage of collagen fibers, reducing the oxidative stress level of photoaged skin, maintaining the integrity of the extracellular matrix (ECM) of the skin, and reducing skin inflammatory reactions, etc., showing good effects. Therefore, the present invention can make full use of the medicinal effects of Chinese herbal medicines, remove the factors affecting safety, improve the convenience of operation, expand the scope of application, and has good effects.

[0031] Specifically, the present invention has the following beneficial effects:

[0032] (1) By establishing a UV-induced mouse skin photoaging model, the present invention studies the protective effects of the traditional Chinese medicine compound preparation on the UV-induced photoaging phenomenon, and further explores its action mechanism.

[0033] In the research of the present invention, first, the protective effects of the original formula of Lujiao San in Comparative Example 1 on mouse skin photoaging were investigated, and 9-week-old Kunming mice (KM mice) were selected for the experiment.

[0034] Obviously, the body repair ability of animals in their youth is stronger than that in middle and old age. In order to better investigate the protective effects of the traditional Chinese medicine compound preparation on skin photoaging, the present invention also selected 11-month-old mice for the experiment. The natural lifespan of KM mice is usually 2 - 3 years, and some literatures also believe that its average lifespan is 1.5 years. The oldest mice that can be purchased locally are 11 months old, which is equivalent to the middle and old age of KM mice. Conducting experiments with them will better reflect the protective effects of the traditional Chinese medicine compound preparation on skin photoaging in older animals.

[0035] In the experimental study, it was observed that the original formula preparation of Lujiao San had obvious protective effects on the skin photoaging of 9-week-old mice. For 11-month-old mice, typical photoaging characteristics appeared after 8 consecutive days of UV irradiation. The epidermis of the dorsal skin of the mice was significantly thickened, and the collagen fibers in the mouse skin tissue were in a loose, disordered, and fragmented state, indicating the establishment of a UV-induced mouse skin photoaging model. And the traditional Chinese medicine compound preparation can effectively reduce the skin photoaging phenomenon caused by UV, making the mouse skin tend to the normal state.

[0036] Regarding oxidative stress, the traditional Chinese medicine compound preparation exhibited significant antioxidant activity. Experimental data showed that the activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) in the skin tissue of the treatment group were significantly increased, while the content of malondialdehyde (MDA) was significantly decreased. These results suggest that this traditional Chinese medicine compound preparation may enhance the body's antioxidant defense capacity by activating the Nrf2 / ARE signaling pathway. Of particular note is the more significant inhibitory effect on the expression of inflammatory factors while reducing oxidative stress indicators, indicating that it may exert a protective effect through multi-target synergy.

[0037] Inflammation response testing results showed that this traditional Chinese medicine compound preparation could significantly reduce the expression levels of pro-inflammatory factors such as α-tumor necrosis factor (TNF-α) and interleukin-6 (IL-6). This finding not only verifies the traditional Chinese medicine theory regarding the "warming and blood-activating" effects of deer antler powder, but also provides an explanation for its anti-inflammatory mechanism. Attached Figure Description

[0038] Figure 1 This is the ultraviolet irradiation modeling and experimental procedure in the embodiments of the present invention;

[0039] Figure 2 This invention provides a comparative example 1 of the protection of mouse skin against photoaging caused by the original deer antler powder formula.

[0040] Figure 3 This invention provides a comparative example 1 showing the effect of the original deer antler powder formula on the growth rate of skin thickness on the back of mice.

[0041] Figure 4 The effect of the original deer antler powder preparation of Comparative Example 1 of this invention on the skin fold score on the back of mice (compared with the Model group, *P<0.05, **P<0.01);

[0042] Figure 5 The effect of the original deer antler powder preparation on the skin tissue structure of mice in Comparative Example 1 of this invention (H&E staining, Masson staining images);

[0043] Figure 6 The effect of the original deer antler powder preparation of the present invention on the collagen volume ratio of the back skin of mice (compared with the Model group, *P<0.05, **P<0.01);

[0044] Figure 7 The effect of the original deer antler powder preparation of Comparative Example 1 of this invention on the oxidative stress level of photoaged skin in mice was (A) SOD; (B) GSH-Px; (C) MDA; (D) 8-oxoG (compared with the Model group, *P<0.05, **P<0.01);

[0045] Figure 8The effects of the original deer antler powder preparation on the extracellular matrix and inflammatory factors of skin cells in KM mice were compared with those of the Model group in the present invention. (A) MMP-3; (B) TNF-α; (C) IL-6; (D) HA; (E) TIMP1 (*P<0.05, **P<0.01) ;

[0046] Figure 9 This invention provides protection against photoaging of mouse skin by a traditional Chinese medicine compound preparation as described in Example 1 of the present invention.

[0047] Figure 10 This invention illustrates the effect of a traditional Chinese medicine compound preparation in Example 1 on the growth rate of skin thickness on the back of mice.

[0048] Figure 11 The effect of the traditional Chinese medicine compound preparation in Example 1 of this invention on the skin fold score on the back of mice (compared with the Model group, *P<0.05, **P<0.01);

[0049] Figure 12 The effect of the traditional Chinese medicine compound preparation in Example 1 of this invention on the skin tissue structure on the back of mice (H&E staining, Masson staining images);

[0050] Figure 13 The effect of the traditional Chinese medicine compound preparation in Example 1 of this invention on the collagen volume ratio of the skin on the back of mice (compared with the Model group, *P<0.05, **P<0.01);

[0051] Figure 14 The effects of the traditional Chinese medicine compound preparation in Example 1 of this invention on the oxidative stress level of photoaged skin in mice were: (A) SOD; (B) GSH-Px; (C) MDA; (D) 8-oxoG (compared with the Model group, *P<0.05, **P<0.01);

[0052] Figure 15 The effects of the traditional Chinese medicine compound preparation in Example 1 of this invention on the extracellular matrix and inflammatory factors of mouse skin cells: (A) MMP-3; (B) TNF-α; (C) IL-6; (D) HA; (E) TIMP1 (Compared with the Model group, *P<0.05, **P<0.01). Detailed Implementation

[0053] The present invention will now be described in further detail with reference to specific embodiments. The given embodiments are merely illustrative of the invention and not intended to limit its scope. The embodiments provided below can serve as a guide for further improvements by those skilled in the art and do not constitute a limitation on the invention in any way.

[0054] Unless otherwise specified, the methods used in the following embodiments are conventional methods, performed according to the techniques or conditions described in the literature in this field or according to the product instructions. Unless otherwise specified, the materials and reagents used in the following embodiments are commercially available.

[0055] The raw materials used in the following examples and comparative examples—deer antler powder, angelica sinensis, asparagus root, atractylodes macrocephala, ampelopsis japonica, chuanxiong rhizome, apricot kernel, cinnamon twig, dried ginger, asarum, aconite root, and angelica dahurica—are all medicinal materials listed in the Chinese Pharmacopoeia. After testing, all indicators met the regulations. The sources of each raw material are as follows: deer antler powder was purchased from Liaoning; atractylodes macrocephala from Anhui; chuanxiong rhizome from Sichuan; apricot kernel from Inner Mongolia; ampelopsis japonica from Hebei; asparagus root from Guangxi; angelica sinensis from Gansu; cinnamon twig from Guangxi; dried ginger from Sichuan; asarum from Shaanxi; and aconite root and angelica dahurica from Henan.

[0056] Example 1

[0057] The preparation method of the traditional Chinese medicine compound extract preparation provided in this embodiment includes the following steps:

[0058] (1) Weigh out 80g each of deer antler powder, angelica and asparagus, and 50g each of atractylodes macrocephala, white peony root, chuanxiong rhizome, apricot kernel, cinnamon twig and dried ginger.

[0059] (2) Each medicinal material is crushed and mixed separately, and then mixed with anhydrous ethanol at a material-to-liquid ratio of 1g:10mL. The mixture is first soaked for 12 hours at room temperature; then it is heated and refluxed at 85℃ for 45 minutes; after cooling to room temperature, the mixture is filtered and the filtrate is retained; the filter residue is heated and refluxed with anhydrous ethanol twice more according to the above method.

[0060] (3) The filtrates were combined and concentrated under vacuum at 45°C using a rotary evaporator. The concentrate was then freeze-dried and pulverized to obtain the lyophilized powder of the alcohol extract of the traditional Chinese medicine compound, which was stored at -20°C for later use. The final extraction rate of the traditional Chinese medicine compound extract was 25.55%.

[0061] (4) A blank solvent was prepared using an anhydrous ethanol:propylene glycol ratio of 3:7 (v / v) for topical application on the skin. 10 mg of the lyophilized powder of the traditional Chinese medicine compound extract was dissolved in 100 mL of the blank solvent to prepare a low-dose traditional Chinese medicine compound extract preparation (LJSMF-L); 20 mg of the lyophilized powder was dissolved in 100 mL of the blank solvent to prepare a high-dose traditional Chinese medicine compound extract preparation (LJSMF-H).

[0062] Comparative Example 1

[0063] Compared with Example 1, Comparative Example 1 used a traditional Chinese medicine formula (the original formula of Deer Antler Powder).

[0064] The preparation method of the original formula of deer antler powder provided in this comparative example includes the following steps:

[0065] (1) Weigh out 60g each of the Chinese medicinal materials deer antler powder and asparagus root, and 30g each of atractylodes macrocephala, white peony root, apricot kernel, asarum, white aconite and angelica dahurica.

[0066] (2) Each medicinal material is crushed and mixed separately, and then mixed with anhydrous ethanol at a material-to-liquid ratio of 1g:10mL. The mixture is first soaked for 12 hours at room temperature; then it is heated and refluxed at 85℃ for 45 minutes; after cooling to room temperature, the mixture is filtered and the filtrate is retained; the filter residue is heated and refluxed with anhydrous ethanol twice more according to the above method.

[0067] (3) The filtrates were combined and concentrated under vacuum at 45°C using a rotary evaporator. The concentrate was then freeze-dried and pulverized to obtain the lyophilized powder of the alcohol extract of the traditional Chinese medicine compound, which was stored at -20°C for later use. The final extraction rate of the traditional Chinese medicine compound extract was 19.83%.

[0068] (4) A blank solvent was prepared by using an anhydrous ethanol:propylene glycol ratio of 3:7 (v / v). 10 mg of the lyophilized powder of the original deer antler powder was dissolved in 100 mL of the blank solvent to prepare a low-dose deer antler powder preparation (LJS-L); 20 mg of the lyophilized powder was dissolved in 100 mL of the blank solvent to prepare a high-dose deer antler powder preparation (LJS-H).

[0069] Comparative Example 2

[0070] Compared with Example 1, Comparative Example 2 used a vitamin E preparation.

[0071] The vitamin E preparation provided in this comparative example is prepared by the following steps:

[0072] (1) Weigh 16.8 mg of the oil-filled liquid contents of commercially available vitamin E capsules (calculated based on a 25% content).

[0073] (2) A blank solvent was prepared by using anhydrous ethanol:propylene glycol at a volume ratio of 3:7. The oily contents of 19.2 mg vitamin E capsules (containing 52% vitamin E) were dissolved in 100 mL of the blank solvent to prepare a vitamin E preparation (VE).

[0074] Examples of animal experimental results

[0075] This study aims to investigate the effects and mechanisms of action of traditional Chinese medicine compound preparations in a mouse skin photoaging model.

[0076] 1. Experimental reagent solution and its preparation method

[0077] This invention first used 9-week-old Kunming mice (KM mice) to investigate the protective effect of the original Deer Antler Powder formula against photoaging of young mouse skin. The preparation method of the drug solution for this animal experiment is as follows:

[0078] Table 1: Drug solutions and their preparation methods used in animal experiments on 9-week-old Kunming mice

[0079]

[0080] The study then used 11-month-old Kunming mice (KM mice) to investigate the protective effect of a traditional Chinese medicine compound extract preparation, modified from the original Deer Antler Powder formula, against photoaging of the skin in middle-aged and older mice. The preparation method of the drug solution for this animal experiment is as follows:

[0081] Table 2: Drug solutions and their preparation methods used in animal experiments on 11-month-old Kunming mice

[0082]

[0083]

[0084] 2. Laboratory animals

[0085] Healthy SPF-grade female KM mice, aged 9 weeks and 11 months, weighing between 34 and 36g, were provided by the Animal Experiment Center of Hubei Provincial Center for Disease Control and Prevention. All animal experimental procedures were strictly performed in accordance with internationally recognized standards and strictly adhered to the Chinese national standard "Guidelines for Ethical Review of Laboratory Animal Welfare" (GB / T 35892—2018) and related regulations.

[0086] 3. Animal experimental methods

[0087] 3.1 Establishment of a mouse model of skin photoaging

[0088] Photoaging of mouse skin was induced by UVA+UVB ultraviolet light irradiation. In a self-made irradiation chamber, two UVB lamps and four UVA lamps were placed on the top, with the mouse backs 30 cm away from the ultraviolet lamps. After hair removal on the mouse backs, the ultraviolet light irradiation experiment was conducted, and the ultraviolet intensity was measured to be 1400 μw / cm² using a UV lux meter. 2 (Radiation dose = UV intensity × time). Preliminary experiments were conducted to determine the minimum erythema dose (MED). In the formal experiment, UV irradiation lasted for a total of 4 weeks, 6 days per week, followed by a 1-day interval. The UV irradiation and experimental procedures are detailed below. Figure 1 During the first week, each irradiation session was 1 MED (total UVA and UVB dose was 504 mJ / cm²). 2 During the second week, each irradiation session was 1.2 MED (604.8 mJ / cm²). 2During the third week, each irradiation session was 1.4 MED (705.6 mJ / cm²). 2 During week 4, each irradiation session was 1.6 MED (806.4 mJ / cm). 2 Mice modeled using this method showed significant photoaging on their backs, such as increased thickness, deeper wrinkles, and sagging. (See...) Figure 2 .

[0089] 3.2 Experimental grouping and drug administration

[0090] Animal experiments were conducted in two phases. First, 9-week-old Kunming mice were used to investigate the protective effect of the original deer antler powder formula against photoaging of young mouse skin. Then, 11-month-old Kunming mice were used to investigate the protective effect of the herbal compound extract formulation of this invention. The purchased mice underwent a one-week acclimatization period, with feeding and free access to water provided every morning. They were then randomly divided into groups of nine mice each, and each group was clearly marked. The grouping methods are shown in Tables 1 and 2, respectively.

[0091] ① First, take a 3×4cm sample from the central area of ​​the back of each group of mice. 2 For the affected area, use depilatory cream to remove most of the hair, then gently shave off the remaining fine hairs with an electric shaver. If new hairs appear, shave them off with an electric shaver for up to one month.

[0092] The control group mice only underwent operation ① and were not exposed to UV radiation.

[0093] After the operation ① was performed on mice in the model group and each drug administration group, they were irradiated with ultraviolet light. After the irradiation was completed, a 6% D-galactose (D-gal) solution (dissolved in 0.9% physiological saline) was injected subcutaneously into the neck. Then, the drug was administered to the hairless area on the back. Mice in the blank group and model group were given a blank solvent without the drug.

[0094] 3.3 Observation Indicators

[0095] 3.3.1 Skin wrinkles and thickness in mice

[0096] During the experiment, close monitoring of the skin on the backs of the experimental mice is necessary, paying particular attention to any adverse reactions such as erythema, blisters, or erosion. If any of these symptoms are observed, UV irradiation should be immediately stopped to avoid further skin damage. Simultaneously, the thickening and loosening of the skin on the backs of the mice, as well as the appearance of deep wrinkles, should be carefully observed. The wrinkle status of the exposed back skin should be graded to allow for quantitative analysis and comparison of the experimental results. The scoring criteria are shown in Table 3.

[0097] Table 3: Skin Wrinkle Status Rating

[0098]

[0099] Skin thickness measurement: Starting from the first day of modeling, the thickness was measured every 3 or 4 days for 4 consecutive weeks. Skin fold thickness was measured by gently pinching the skin on the back of the mouse and using digital calipers positioned midway between the neck and rump.

[0100] 3.3.2 Pathological and Histological Observation

[0101] The day after administration, mice were euthanized by cervical dislocation under ether anesthesia. A 0.5cm x 0.5cm piece of skin was then removed from the midline of the mouse's back. During specimen collection, the skin was kept in its natural state as much as possible, avoiding stretching or deformation. When using forceps, the skin edges were gently grasped and placed on a piece of filter paper of approximately the same size. This prevented the skin from curling and facilitated subsequent operations. Care was taken to avoid damaging or deforming the skin when using forceps.

[0102] Fixed with 4% paraformaldehyde at 4°C for 48 hours, then embedded in paraffin. Before embedding, the tissue was dehydrated in an automatic dehydrator, and then placed in a paraffin embedding machine for further embedding. Before staining, the paraffin sections were dewaxed using a gradient dewaxing process: soaked in xylene I for 20 minutes, xylene II for 20 minutes, anhydrous ethanol I for 5 minutes, anhydrous ethanol II for 5 minutes, and 75% ethanol for 5 minutes, followed by washing with water. Finally, H&E staining and Masson staining were performed using the appropriate staining reagents. H&E staining was used to observe skin tissue structure, and Masson staining was used to observe collagen fibers. After staining, the sections were mounted with neutral resin.

[0103] Observation and imaging were performed under a microscope. Epidermal thickness, i.e., the distance perpendicular to the basal layer to the stratum corneum, was measured using CellSens Standard software. Changes in skin tissue structure, the arrangement of collagen and elastic fibers, and tissue structure could be observed through different sections. (See...) Figure 2 and Figure 3 .

[0104] 3.3.3 Determination of antioxidant enzymes, MDA, ROS, 8-oxoG and HA content in mouse skin

[0105] Take about 0.1g of mouse skin tissue, cut it into small pieces, put it into a pre-cooled cryovial containing 900μL of physiological saline, add 3-4 3mm grinding beads, homogenize it thoroughly on a homogenizer at 4℃, then transfer it to a low-temperature high-speed centrifuge and centrifuge at 12000g for 15min at 4℃. Use a 1mL sterile syringe to draw the supernatant into a 1.5mL EP tube, centrifuge again at 4℃ for 15min, draw the supernatant into an EP tube, and freeze at -20℃ for later use.

[0106] The content was determined using a kit for superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), catalase (CAT), reactive oxygen species (ROS), malondialdehyde (MDA), 8-oxoguanine (8-oxoG), and hyaluronic acid (HA).

[0107] 3.3.4 Determination of MMP-3, TIMP1, TNF-α, and IL-6 gene expression in mouse skin

[0108] 3.3.4.1 Total RNA Extraction

[0109] Total RNA was extracted from mouse tissues using Trizol reagent. RNA concentration and purity (OD 260 / OD 280, OD 260 / OD 230) were determined using an ultra-micro nucleic acid and protein analyzer. RNA degradation and contamination were detected by 1% agarose gel electrophoresis. The obtained RNA was stored at -80°C and used for reverse transcription to obtain cDNA.

[0110] 3.3.4.2 RNA reverse transcription to obtain cDNA

[0111] RNA reverse transcription reaction was performed using Thermo Scientific. TM The RevertAid First Strand cDNASynthesis Kit (K1622) is used, and all procedures are performed in an icebox. The specific steps are as follows:

[0112] (1) Reverse transcription: Mix all reagents according to Table 4, react immediately after mixing, and react at 42℃ for 60 min. Then heat to 72℃ for 5 min to terminate the reaction. After the reaction, store at -20℃ for a short period and at -80℃ for a long period.

[0113] Table 4: Reverse Transcription Reaction System

[0114]

[0115]

[0116] 3.3.4.3 Real-time quantitative polymerase chain reaction

[0117] Real-time quantitative polymerase chain reaction using Hieff The reaction system for the Universal Blue qPCR SYBRGreen Master Mix kit is shown in Table 5.

[0118] Table 5: Real-time PCR reaction system

[0119]

[0120] The reaction procedure is as follows: pre-denaturation at 95℃ for 2 min, denaturation at 95℃ for 10 s, 60℃ for 3 s, 40 cycles, with the melting curve stage set by the instrument's default settings.

[0121] Primers for real-time quantitative polymerase chain reaction were designed using the NCBI Primer-BLAST webpage, and the primer sequences are shown in Table 6.

[0122] Table 6: Primer Sequences

[0123]

[0124]

[0125] 3.4 Statistical Analysis

[0126] Each test experiment was repeated three times. The results were analyzed using GraphPad Prism 5.01-tril statistical software. Data for each experimental group are expressed as Mean±SD. The t-test was used to compare the data between each drug administration group and the model group. The difference was considered statistically significant when P<0.05.

[0127] 4. Experimental Results

[0128] Animal experiments were conducted in two phases. First, 9-week-old Kunming mice were used to investigate the protective effect of the original deer antler powder formulation (LJS) against photoaging of young mouse skin. Then, 11-month-old Kunming mice were used to investigate the protective effect of the traditional Chinese medicine compound extract formulation (LJSMF) of this invention against photoaging of middle-aged and older mice.

[0129] 4.1 The protective effect of the original formula of deer antler powder on photoaging of mouse skin

[0130] 4.1.1 Effects of the original formula of Deer Antler Powder on the photoaged skin condition of mice

[0131] like Figure 2 As shown, the skin of the blank group (Blank mice) was smooth, undamaged, wrinkle-free, and elastic. After 8 days of UVB irradiation, the skin of the model group (Model mice) became dry, erythematous, and rough, resembling leather, with slight surface damage and significant skin laxity. Continued observation revealed that the vitamin E group (VE), the low-dose group (LJS-L) and the high-dose group (LJS-H) of the original deer antler powder formula significantly improved UV-induced skin morphological changes and significantly reduced skin lesions in mice, alleviating symptoms such as roughness and laxity. This indicates that the original deer antler powder formula can reduce skin wrinkles, restore a rosy appearance, reduce erythema, and alleviate damage. 4.1.2 Effects of the original deer antler powder formula on the thickness of photoaged skin in mice and skin condition scores.

[0132] The results are as follows Figure 3 As shown, compared with the model group, the rate of increase in skin thickness in mice in the high / low dose groups of the original deer antler powder preparation was significantly reduced after day 13; Figure 4 As shown, the skin fold scores of each treatment group gradually decreased after day 17. In particular, the high-dose group showed a lower increase in wrinkles on day 8 compared to the model group, and by day 28, the score had dropped to nearly 2 points, significantly lower than the model group. Figure 3 and Figure 4 This indicates that the original formula of Lujiaosan has a significant effect on delaying the thickening and wrinkles caused by photoaging of the skin, and the high-dose effect of the original formula of Lujiaosan is similar to that of vitamin E.

[0133] 4.1.3 Effects of the original formula of Deer Antler Powder on the structure of photoaged skin tissue in mice

[0134] Morphological changes in mouse skin were observed using H&E staining. Figure 5 As shown in the H&E staining images, the epidermis of the skin tissue in the Blank group was thinner. The epidermis of the Model group mice was thickened, with disordered epidermal and dermal structures and significant inflammatory cell infiltration. However, the thickening and structural changes of the epidermis in the VE, LJS-L, and LJS-H groups were all less severe than in the model group, indicating that the original formula of Lujiaosan significantly improved the tissue structure of UV-induced photoaged skin in mice.

[0135] Masson staining was used to observe the morphological changes of collagen fibers in mouse skin tissue; in Masson staining, collagen fibers were stained blue. Figure 5 As shown in the Masson staining images, the collagen fibers in the dermis of mice in the Blank, VE, and LJS-H groups were tightly packed, neatly arranged, and generally normal in shape. In the Model group, the collagen fibers in the dermis were less abundant, disordered, and less distributed. Figure 6 The collagen volume ratio showed that the collagen fiber content in the Model group was significantly lower than that in the Blank group; however, there were no significant differences between the various treatment groups and the Blank group, and the collagen fiber content in the VE group and LJS-H group was significantly higher than that in the Model group. This indicates that the original formula of Lujiaosan can improve the damage to collagen fibers caused by ultraviolet-induced skin photoaging.

[0136] 4.1.4 The regulatory effect of the original formula of deer antler powder on the oxidative stress level of photoaged skin in mice.

[0137] like Figure 7 As shown in Figures A and B, the SOD and GSH-Px activities in the VE, LJS-L, and LJS-H groups were significantly higher than those in the Model group. 8-oxoG is a biomarker of DNA oxidative damage in vivo, and MDA is an important biomarker of oxidative stress in vivo. Figure 7 As shown in C and D, the MDA content in each treatment group was lower than that in the Model group, but the difference was not statistically significant, while the 8-oxoG content was significantly lower than that in the Model group. This result indicates that the original formula of Lujiaosan can effectively alleviate oxidative stress damage in photoaged skin of mice by enhancing the activity of antioxidant enzymes and reducing DNA damage.

[0138] 4.1.5 Regulation of extracellular matrix and inflammation levels in photoaged mouse skin by the original formula of deer antler powder

[0139] like Figure 8 As shown, the gene expression of matrix metalloproteinase-3 (MMP-3) and the inflammatory factor TNF-α in the VE group, LJS-L group, and LJS-H group was significantly lower than that in the Model group; the expression of the inflammatory factor IL-6 in the LJS-H group was significantly lower than that in the Model group; hyaluronic acid (HA), one of the main matrix components of skin tissue, showed significantly higher HA content in each treatment group compared to the Model group; the expression of tissue inhibitor of metalloproteinases 1 (TIMP1) showed no significant difference among the treatment groups compared to the Model group. These results indicate that deer antler powder plays a significant role in maintaining the matrix stability of skin tissue and exhibits certain efficacy in anti-photoaging and anti-inflammation.

[0140] 4.2 Protective effect of traditional Chinese medicine compound on photoaging of mouse skin

[0141] 4.2.1 Effects of Traditional Chinese Medicine Compound on Photoaged Skin Condition in Mice

[0142] like Figure 9 As shown, the skin of the normal control group (Blank mice) remained intact and smooth. After long-term UV irradiation, the skin of the model group (Model) exhibited typical photoaging characteristics such as dryness, peeling, diffuse erythema, and coarse wrinkles. After intervention with different doses of the traditional Chinese medicine compound extract preparation (LJSMF) of this invention, the epidermal integrity of the treated groups was significantly restored, and the skin surface roughness and elasticity were significantly improved. This indicates that the traditional Chinese medicine compound preparation of this invention can reduce skin wrinkles in middle-aged and elderly mice, restore a rosy appearance, reduce erythema, and alleviate damage.

[0143] 4.2.2 Effects of Traditional Chinese Medicine Compound on Photoaged Skin Thickness and Skin Condition Scoring in Mice

[0144] The results of the experiment are as follows Figure 10 and Figure 11As shown, compared with the Model group, the skin thickness growth rate and wrinkle score of mice in the LJSMF treatment group were significantly reduced, especially on day 10, when the skin thickness growth rate of the LJSMF-L group decreased by 180%, significantly higher than that of the Model group. Furthermore, during the observation period from day 8 to day 20, the wrinkle score decreased from 4 to 3, and by day 24, it was significantly lower than that of the Model group. This indicates that the traditional Chinese medicine compound preparation of the present invention can effectively slow down the increase in skin thickness and wrinkle formation caused by photoaging in middle-aged and elderly mice.

[0145] 4.2.3 Effects of Traditional Chinese Medicine Compound on the Structure of Photoaged Skin Tissue in Mice

[0146] like Figure 12 As shown in the H&E staining images, the skin tissue and cell layer in the Blank group were thinner. In the Model group, the epidermis was thickened, the epidermal and dermal structures were disordered, and inflammatory cell infiltration was significant. However, the skin lesions in the LJSMF-L and LJSMF-H groups were less severe than in the Model group, indicating that the traditional Chinese medicine compound preparation of this invention significantly improved the photoaging morphology of skin in middle-aged and older mice induced by ultraviolet radiation.

[0147] The model group mice showed significant epidermal atrophy, disordered collagen fibers in the dermis, and inflammatory cell infiltration.

[0148] like Figure 12 As shown in the Masson staining images, the collagen fibers in the dermis of mice in the Blank, LJSMF-L, and LJSMF-H groups were tightly arranged, neatly aligned, and generally normal in shape. In the model group, the epidermis of the skin was significantly atrophied, and the collagen fibers in the dermis were fewer and more disordered. Figure 13 As shown, compared with the Blank group, the collagen fiber content in the Model group was significantly reduced; compared with the Model group, the collagen fiber content in the LJSMF-L and LJSMF-H groups was significantly increased. This indicates that the traditional Chinese medicine compound preparation can improve the damage to collagen fibers in the skin of middle-aged and aged mice induced by ultraviolet radiation, further demonstrating that the traditional Chinese medicine compound preparation of the present invention has a certain protective effect against photoaging of mouse skin.

[0149] 4.2.4 The regulatory effect of traditional Chinese medicine compound on oxidative stress level in photoaged skin of mice

[0150] like Figure 14 As shown, in both groups treated with LJSMF, the activities of antioxidant enzymes SOD, GSH-Px, and CAT were significantly higher than those in the model group; the contents of reactive oxygen species (ROS) and MDA in the skin tissue of the LJSMF-treated group were significantly lower than those in the model group. This indicates that the traditional Chinese medicine compound preparation of the present invention can effectively alleviate oxidative stress damage to photoaged skin in middle-aged and aged mice by increasing antioxidant enzyme activity, reducing lipid peroxidation, and decreasing reactive oxygen species.

[0151] 4.2.5 Regulation of extracellular matrix and inflammation levels in photoaged skin of mice by traditional Chinese medicine compound

[0152] like Figure 15 As shown, MMP-3 gene expression was significantly lower in the LJSMF-L group compared to the Model group, while TIMP1 expression showed no significant difference, indicating that LJSMF can alleviate collagen degradation in skin tissue. The gene expression of inflammatory factors TNF-α and IL-6 was significantly lower in both the LJSMF-L and LJSMF-H groups compared to the Model group, indicating that LJSMF can significantly reduce the inflammatory response in photoaged skin tissue. Compared to the Model group, the HA content in the skin tissue of the LJSMF-treated groups was significantly increased. These experimental results demonstrate that the traditional Chinese medicine compound preparation of this invention has a significant effect on maintaining the matrix integrity of skin tissue and also exhibits a positive effect in reducing inflammation in photoaged skin of middle-aged and elderly mice.

[0153] The above results demonstrate that the traditional Chinese medicine compound preparation of this invention also exhibits good protective effects in a mouse skin photoaging model, particularly in slowing down the increase in skin thickness caused by photoaging, reducing wrinkle formation and collagen fiber damage, lowering the oxidative stress level of photoaged skin, maintaining the integrity of the skin extracellular matrix (ECM), and alleviating skin inflammation. More importantly, this invention removes three traditional Chinese medicinal materials prohibited in cosmetics: Asarum, Aconitum carmichaelii, and Angelica dahurica, eliminating factors that could affect safety, improving ease of operation, and expanding the scope of application.

[0154] The present invention has been described in detail above. Those skilled in the art will recognize that the invention can be practiced in a wide range of ways with equivalent parameters, concentrations, and conditions without departing from its spirit and scope. While specific embodiments have been provided, it should be understood that further modifications can be made to the invention. In summary, according to the principles of the invention, this application is intended to include any changes, uses, or improvements to the invention, including modifications made using conventional techniques known in the art that depart from the scope disclosed herein.

Claims

1. A traditional Chinese medicine composition for resisting skin photoaging, characterized in that, The anti-skin photoaging traditional Chinese medicine compound preparation is prepared from the raw materials in the following weight parts: antler velvet 70-90 parts, angelica 70-90 parts, asparagus 70-90 parts, white atractylodes 50-60 parts, white cork 50-60 parts, Szechuan lovage 50-60 parts, bitter apricot kernel 50-60 parts, cassia twig 50-60 parts, and dried ginger 50-60 parts.

2. The anti-skin photoaging traditional Chinese medicine composition according to claim 1, characterized in that: The anti-skin photoaging traditional Chinese medicine compound preparation is prepared from the raw materials in the following weight parts: antler velvet 70-90 parts, angelica 70-90 parts, asparagus 70-90 parts, white atractylodes 50-60 parts, white cork 50-60 parts, Szechuan lovage 50-60 parts, bitter apricot kernel 50-60 parts, cassia twig 50-60 parts, and dried ginger 50-60 parts.

3. A traditional Chinese medicine compound preparation for resisting skin photoaging, characterized in that, The anti-skin photoaging traditional Chinese medicine compound preparation is prepared from the raw materials in the following weight parts: antler velvet 70-90 parts, angelica 70-90 parts, asparagus 70-90 parts, white atractylodes 50-60 parts, white cork 50-60 parts, Szechuan lovage 50-60 parts, bitter apricot kernel 50-60 parts, cassia twig 50-60 parts, and dried ginger 50-60 parts.

4. The anti-skin photoaging traditional Chinese medicine compound preparation according to claim 3, characterized in that: The anti-skin photoaging traditional Chinese medicine compound preparation is prepared from the raw materials in the following weight parts: antler velvet 70-90 parts, angelica 70-90 parts, asparagus 70-90 parts, white atractylodes 50-60 parts, white cork 50-60 parts, Szechuan lovage 50-60 parts, bitter apricot kernel 50-60 parts, cassia twig 50-60 parts, and dried ginger 50-60 parts.

5. The preparation method of the anti-skin photoaging traditional Chinese medicine compound preparation according to claim 4, comprising the following steps: S1, crushing the raw materials and placing them in anhydrous ethanol for extraction, and collecting the extract; S2, sequentially performing vacuum concentration and freeze-drying on the extract to obtain an alcohol extract freeze-dried powder.

6. The method for preparing the traditional Chinese medicine compound preparation for anti-skin photoaging according to claim 5, characterized in that: The ratio of the raw materials to the anhydrous ethanol is 1g: (6-15) mL; The extraction step is as follows: first mixed at room temperature for 12-24 hours, and then heated and refluxed at 82-90°C for 45-60 minutes; preferably, the number of times of heating and refluxing is 2-3 times.

7. The anti-skin photoaging traditional Chinese medicine compound preparation of claim 1 or 2, the anti-skin photoaging traditional Chinese medicine compound preparation of claim 3 or 4, or the anti-skin photoaging traditional Chinese medicine compound preparation prepared by the preparation method of claim 5 or 6, is used in any one of the following A1-A2: A1, preparing a cosmetic for anti-skin photoaging; A2, non-therapeutic anti-skin photoaging.

8. Use according to claim 7, characterized in that: The anti-skin photoaging is manifested in any one of the following 1)-7): 1) improving the characteristics of skin photoaging or relieving the damage of skin photoaging; 2) slowing down the thickness increase and wrinkle formation caused by skin photoaging; 3) improving the inflammatory cell infiltration state of skin photoaging; 4) improving the damage of collagen fibers of skin photoaging; 5) reducing the oxidative stress damage of photoaged skin; 6) maintaining the integrity of the extracellular matrix of the skin; 7) reducing inflammation caused by skin photoaging.

9. A cosmetic product, characterized by, The anti-skin photoaging traditional Chinese medicine compound preparation of claim 1 or 2, the anti-skin photoaging traditional Chinese medicine compound preparation of claim 3 or 4, or the anti-skin photoaging traditional Chinese medicine compound preparation prepared by the preparation method of claim 5 or 6.

10. The cosmetic product of claim 9, wherein: The cosmetic is a skin care product, further comprising any one of the following: cosmetic water, essence, emulsion, cream, spray, and tincture.

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