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Preparation method of virus-free seedlings of sweet potato

A technology for virus-free seedlings and sweet potatoes, which is applied in the field of sweet potato seedling cultivation, can solve the problems of complicated domestication and transplanting operations of virus-free seedlings, difficult peeling of stem tips, poor detoxification effect, etc., and achieves good detoxification effect and reduces cultivation. time, high regeneration rate

Active Publication Date: 2014-10-01
QINGDAO AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The invention provides a method for preparing virus-free sweet potato seedlings, which can solve the problems in the prior art that the virus-free effect is poor, the stem tip is not easy to strip, the regeneration rate is low, the operation of acclimating and transplanting the virus-free seedlings is cumbersome, and the survival rate is low. slow growth problem

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  • Preparation method of virus-free seedlings of sweet potato
  • Preparation method of virus-free seedlings of sweet potato
  • Preparation method of virus-free seedlings of sweet potato

Examples

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Effect test

Embodiment 1

[0046] The preparation method of sweet potato virus-free seedling of the present invention specifically comprises the following steps:

[0047] 1. Treatment of sweet potato cubes before germination:

[0048] (1) After harvesting in autumn, select the currently popular sweet potato varieties Xushu 22, Yanshu 25, and Shangshu 19 (commercially available varieties) without moth-eaten and damage-free potato pieces, and expose them to the sun for 2-3 days (No. One has a bactericidal effect; the second can promote the germination of potato pieces).

[0049] (2) Prepare ripe bananas, put the sun-exposed potato pieces and ripe bananas together in a plastic storage box, close the lid, and store them in the laboratory for 3-5 days (at room temperature not lower than 13°C) (Use mature Bananas release ethylene, which promotes the germination of the potato cubes. Because the newly harvested potato cubes germinate slowly, resulting in rotten potatoes).

[0050] 2. Germination:

[0051]...

Embodiment 2

[0077] The preparation method of sweet potato virus-free seedling described in the present embodiment specifically comprises the steps:

[0078] 1. Treatment of sweet potato cubes before germination:

[0079] (1) After harvesting in autumn, select sweet potato varieties Gaoxi No. 14 (commercially available variety) without insects and damage, and expose them to the sun for 2-3 days (the first has a bactericidal effect; the second can promote the growth of the potato pieces). bud).

[0080] (2) Prepare ripe bananas, put the sun-exposed potato pieces and ripe bananas together in a plastic storage box, close the lid, and store them in the laboratory for 3-5 days (at room temperature not lower than 13°C) (Use mature Bananas release ethylene, which promotes the germination of the potato cubes. Because the newly harvested potato cubes germinate slowly, resulting in rotten potatoes).

[0081] 2. Germination:

[0082] Wash the above-mentioned potato pieces with clean water, pu...

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Abstract

The invention provides a preparation method of virus-free seedlings of sweet potato. The method comprises the following steps: placing sweet potato blocks that are exposed under the sun and matured banana together for 3 to 5 days; planting the sweet potatoes in sandy soil to accelerate the germination in a culture box; setting relatively high temperature; picking 1-2cm top end of the seedling based on the length; cutting off the visual leaves; disinfecting the surface; rinsing with sterile water; picking off stem tip with 1 to 2 leaf primordium; inoculating to the corresponding culture medium to respectively induce differentiation of adventitious buds and generation of somatic embryogenesis; performing virus detection to a regeneration plant; performing subculture for the virus-free seedling; hardening-seedling; directly planting into a plastic greenhouse; performing flood irrigation with more water; preventing direct exposing under sun at noon in the first two weeks after transplanting; applying urea three weeks after transplanting; watering to obtain virus-free seedlings. According to the preparation method of the virus-free seedlings of sweet potato, the virus-free test-tube plantlets are acclimated and then directly planted into the plastic greenhouse, thus the survival rate is obviously raised, and the operation processes are decreased; the process of acclimating in an acclimating room is saved, and as a result, labor, materials and property are saved.

Description

technical field [0001] The invention belongs to the technical field of sweet potato seedling cultivation, and in particular relates to a method for preparing virus-free sweet potato seedlings. Background technique [0002] Sweet potato is one of the four major food crops in my country. Sweet potato is a vegetatively propagated crop and is susceptible to virus infection during cultivation. Once infected, it is difficult to remove. The accumulation of viruses can cause the degeneration of varieties, the reduction of quality and yield, and even the loss of commodity value, causing serious harm to sweet potato production. So far, there are no practical sweet potato varieties with high resistance to viral diseases at home and abroad, and there are no efficient pesticides for preventing and treating viral diseases. The distribution of viruses in plants is uneven, the closer to the root tip and shoot tip, the lower the virus density; conversely, the higher. [0003] The meristem ...

Claims

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Application Information

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IPC IPC(8): A01G1/00A01H4/00
Inventor 王晶珊王鹏纪瑞瑞隋炯明乔利仙郭宝太孙世孟
Owner QINGDAO AGRI UNIV
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