Kit for rapidly detecting bordetella pertussis and detection method

Abstract

The invention discloses a kit for rapidly detecting bordetella pertussis and a detection method thereof. A specific probe is combined with a high-tolerance polymerase technology, a sample can be directly amplified after simple heating treatment, a nucleic acid extraction step is not needed, and the sample treatment time of a pertussis sample is shortened. The components used for detecting bordetella pertussis are prepared into a dry powder preparation, so that the thermal stability of the kit is remarkably improved, and the kit can be used for normal-temperature transportation. The single-tubepackaging of the multi-component dry powder reduces the step of liquid preparation. Experimental results prove that the kit and the detection method thereof are strong in specificity, high in sensitivity and simple to operate, and can be used for directly, quickly and accurately detecting the bordetella pertussis.

Description

technical field [0001] The invention relates to the technical field of pathogenic nucleic acid detection, in particular to a kit for rapidly detecting pertussis bacillus and a detection method thereof. Background technique [0002] pertussis ( Bordetella Pertussis , BP) is the pathogenic bacterium of whooping cough. The incubation period of whooping cough is 1-2 weeks. In the early stage of onset (catarrhal stage), there is only mild cough. At this time, the bacteria multiply on the mucous membrane of the trachea and bronchi and are discharged with droplets, which is the most contagious. . Paroxysmal spasmodic cough (spasmodic cough period) occurs after 1 to 2 weeks. At this time, the bacteria release toxins, causing the cilia of the mucosal epithelial cells to lose control, and a large amount of viscous secretions cannot be discharged, which stimulates the receptors in the mucosa to produce a strong spasmodic cough. It presents a special high-pitched cock-crow-like roar, ...

AI Technical Summary

Problems solved by technology

[0003] At present, the more common clinical methods for pertussis diagnosis are culture method and serological screening, but it is difficult to isolate and culture pertussis bacilli, and the culture period is long, while serological screening has the defects of low sensitivity and low specificity, these factors Limit the clinical application of the above two detection methods of B. pertussis

Although molecular nucleic acid diagnostic technology has greatly accelerated clinical testing in recent years, the operation is still cumbersome and time-consuming in the process of sample processing

Moreover, the existing Bacillus pertussis nucleic acid detection technology requires cumbersome steps to extract the nucleic acid in the pathogen to meet the requirements of the PCR reaction system, and it needs to complete the preparation of multiple tubes during use, which is inconvenient to use

In addition, the existing Bacillus pertussis nucleic acid detection kits need to be kept strictly below -18°C

Its transportation relies on cold chain material transportation, and transportation costs and storage are inconvenient

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