Drug-loaded egg protein microspheres and preparation method thereof

A technology for egg protein and egg white, which is applied in the field of drug controlled release, can solve the problems of restricting production and application, restricting large-scale production, complex extraction process, etc., achieving controllable particle size distribution, and improving encapsulation and controlled release effects. , the effect of good dispersion of microspheres

Active Publication Date: 2014-10-08
CHENGDU UNIVERSITY OF TECHNOLOGY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the raw material cost of ovalbumin is relatively expensive, at least 2000 yuan / g, and the extraction process is complicated, so its large-scale production is limited.
[0005] The invention patent with the application number 200910066561.0 discloses protein microspheres loaded with doxorubicin using human serum albumin as the carrier. Human serum albumin is used as the raw material and the price is relatively cheap, about 500 yuan / g, but the source is limited and the extraction process is also complicated. limit its production and application
[0006] The invention patent with application number 200810053361.7 discloses a preparation method of a new dosage form of porcine serum immunoglobulin microspheres, using porcine serum immunoglobulin as raw material, the preparation of this protein microspheres reduces the cost of raw materials to a certain extent, but the same Facing problems such as complex extraction process and limited application

Method used

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  • Drug-loaded egg protein microspheres and preparation method thereof
  • Drug-loaded egg protein microspheres and preparation method thereof
  • Drug-loaded egg protein microspheres and preparation method thereof

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preparation example Construction

[0038] The preparation method of drug-loaded egg protein microspheres of the present invention has the following steps:

[0039] a, the preparation of oily phase: surfactant is mixed with oily component, and wherein, described surfactant is the mixing of Siban type surfactant and Tween type surfactant, and the hydrophilic-hydrophobic balance value of surfactant is 4 ~10, the oily component is liquid paraffin, olive oil or sunflower oil;

[0040] b. Emulsification: Add the mixture of egg white and medicine to the oil phase and stir to emulsify to form a W / O phase to obtain an emulsion. The stirring time is 15-50 minutes, and the rotation speed during stirring is not higher than 1000r / min;

[0041] c. Curing: the emulsion is directly heated and solidified or cross-linked and solidified by adding an aldehyde curing agent to obtain a cured liquid, wherein the aldehyde curing agent is glutaraldehyde or vanillin;

[0042] d, demulsification: the solidified liquid is mixed with an e...

Embodiment 1

[0051] Take by weighing Span-80 (Span-80) and Tween-60 (Tween-60) that mass ratio is 5:4, wherein the quality of Span-80 is 1.0g, and the HLB value of emulsifier is 9.1, and 47g The liquid paraffin was mixed and stirred uniformly for 15min, poured into the mixed solution the mixed solution of 10g egg white liquid (12% protein content) and 80mg gentamycin sulfate, continued to stir for 30min, the stirring speed was 180r / min, after that the mixed solution Stir in a constant temperature water bath at 80°C for 15 minutes, and the protein solidifies into balls during the heating process. Take out the above mixed solution from the water bath, add petroleum ether equal to the volume of the mixed solution for demulsification, stir for 15 minutes, separate the water and oil phases and centrifuge, then wash with isopropanol and centrifuge 3 times to prepare protein microspheres and The obtained samples were stored freeze-dried.

[0052] The scanning electron micrographs of the drug-loa...

Embodiment 2

[0054] Take by weighing Span-80 (Span-80) and Tween-80 (Tween-80) that mass ratio is 5:3, wherein the quality of Span-80 is 1.0g, and the HLB value of emulsifier is 8.3, and 47g Olive oil was mixed and evenly stirred for 15min, poured into the mixed solution the mixed solution of 10g egg white liquid (12% protein content) and 160mg berberine hydrochloride, continued to stir for 30min, and the stirring speed was 160r / min, after which the mixed solution was Stir in a constant temperature water bath at 80°C for 15 minutes, and the protein solidifies into balls during the heating process. Take out the above mixed solution from the water bath, add petroleum ether equal to the volume of the mixed solution for demulsification, stir for 15 minutes, separate the water and oil phases and centrifuge, then wash with isopropanol and centrifuge 3 times to prepare protein microspheres and The obtained samples were stored freeze-dried.

[0055] The scanning electron micrographs of the drug-l...

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Abstract

The invention relates to drug-loaded egg protein microspheres and a preparation method thereof, belonging to the field of drug controlled release. The invention aims at solving the technical problem of providing the drug-loaded egg protein microspheres and the preparation method thereof. A vector of the drug-loaded egg protein microspheres is egg protein. The preparation method comprises the following steps: mixing a surfactant with an oily component to obtain an oil phase; adding a mixed liquor of the egg protein and drugs to the oil phase to stir and emulsify, and then directly heating and solidifying or adding an aldehyde curing agent to carry out crosslinking solidification; demulsifying by using a petroleum ether after solidification is ended; and cleaning and centrifuging by using isopropyl alcohol, and carrying out freeze-drying, so as to obtain the drug-loaded egg protein microspheres. The microspheres disclosed by the invention have good biocompatibility and biological activity; the particle sizes are 0.2-20mu m, and can be adjusted according to different solidification methods; and the microspheres are good in controlled release effect, are applied to a plurality of drug-delivery ways, such as injection, oral administration and the like, and are wide in material source, low in price, simple and feasible in preparation process and low in production cost.

Description

technical field [0001] The invention relates to drug-loaded egg protein microspheres and a preparation method thereof, belonging to the field of drug controlled release. Background technique [0002] Controlled release of drugs is a new route of drug administration. Generally, polymer materials are used as carriers or media to make certain preparations. Time to maintain a relatively balanced state, so as to achieve better drug release and therapeutic effect. Compared with the traditional way of drug administration, it slows down the sudden release of the drug, avoids the large fluctuation of the blood drug concentration to a certain extent, not only enables the long-term and stable release of the drug, but also improves the utilization rate of the drug. Drug controlled release has developed rapidly in the past 20 years. It is not limited to the control of drug release rate and the horizontal development of drug utilization. big progress. An important development direction...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K47/42A61K9/16
Inventor 李峻峰侯毅何苗苗黄露梅高阳邹琴赖雪飞张佩聪游杨
Owner CHENGDU UNIVERSITY OF TECHNOLOGY
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