Desalting and concentrating of oligopeptide solution by nano filtering technology

A technology of oligopeptide and solution, which is applied in the field of protein and peptide purification and concentration, to achieve the effect of simple process, good effect and low energy consumption

Inactive Publication Date: 2003-01-22
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In China, the desalination and concentration of oligopeptide solutions using nanofiltration technology has not been reported

Method used

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  • Desalting and concentrating of oligopeptide solution by nano filtering technology
  • Desalting and concentrating of oligopeptide solution by nano filtering technology

Examples

Experimental program
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Effect test

Embodiment 1

[0016] Soybeans are used as raw materials to be processed, deoiled, degreased, water extracted, bean dregs removed, acid precipitated, whey removed, separated to obtain soybean protein, and dissolved in water to obtain a protein solution. Add sodium hydroxide solution to adjust the pH value, add protease to carry out enzymatic hydrolysis reaction, control the degree of hydrolysis, heat to inactivate the enzyme, centrifuge and filter to obtain the filtrate, which is the oligopeptide solution. Use the polyamide nanofiltration membrane to desalinate by intermittently adding deionized water to the concentrated solution circulation mode, the operating pressure is 1.5-1.7MPa, the operating temperature is 40-50°C, stop adding deionized water after the desalination rate reaches 90%, and concentrate to 30 % (W / W). The desalted concentrated solution is spray-dried to obtain the soybean peptide powder.

Embodiment 2

[0018] Corn gluten flour (CGM) is obtained by drying the protein waste water separated from starch in the process of corn wet milling with corn as raw material. After adding water to CGM, heat it to 95°C and keep it warm to obtain a protein solution. Add NaOH solution to adjust the pH value. , add protease to carry out enzymatic hydrolysis reaction, control the degree of hydrolysis, heat to inactivate the enzyme, centrifuge, and suction filter to obtain the filtrate, which is the oligopeptide solution. Carry out desalination and concentration with polyethersulfone nanofiltration membrane, desalination and concentration mode are the same as embodiment 1, operating pressure 1.6~1.8MPa, operating temperature 40~50 ℃, desalination rate is 95%, corn peptide solution is concentrated to 35% (W / W). After desalination, the concentrated solution is spray-dried to obtain the product corn peptide powder.

Embodiment 3

[0020] Casein phosphopeptide (CPP) is a phospho-seryl-containing peptide obtained from casein as a raw material, hydrolyzed by enzymes, and then separated and purified. Add water to the casein to obtain a casein solution, add Alcalase enzyme to carry out enzymatic hydrolysis reaction, and use the pH-stat method to control the degree of hydrolysis (DH) = 20%. Inactivate the enzyme, after centrifugation and suction filtration, the filtrate is obtained as a casein oligopeptide solution, which is desalted and concentrated with a sulfonated polysulfone nanofiltration membrane. The desalination and concentration method is the same as in Example 1. At ~45°C, the desalination rate is 90%, and the casein phosphopeptide solution is concentrated to 40% (W / W). After desalination, the feed solution is spray-dried to obtain casein phosphopeptide powder.

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Abstract

The present invention relates to nano filtering technology of desalting and concentrating oligopeptide solution, and belongs to the technology of purifying and concentrating protein and peptide. Milkprotein, silk protein, soybean protein, cereal protein, egg protein, meat protein, fish protein and cotton seed protein material is treated to obtain protein solution; the protein solution is temperature and pH regulated, hydrolyzed, via enzyme process or acid process to reach required degree of hydrolysis; and through centrifugation and filtering, oligopeptide solution is obtained. The oligopeptide solution is desalted and concentrated and the concentrated solution is spray dried to obtain the oligopeptide powder product soybean peptide, corn peptide, silk peptide, etc.

Description

technical field [0001] The invention relates to desalination and concentration of oligopeptide solution by applying nanofiltration technology, and belongs to the technical field of protein and peptide purification and concentration. Background technique [0002] The hydrolysis process of protein is that the protein is first hydrolyzed into macromolecular peptides, then small molecular peptides, and finally hydrolyzed into free amino acids. Small molecular peptides with relatively short peptide chains are called oligopeptides. In the past, it was believed that protein was absorbed in the body after being hydrolyzed into amino acids by proteases in the body, but recent studies have shown that protein is not completely absorbed in the form of amino acids after digestion in the body, but in the form of oligopeptides, and in the form of oligopeptides. Peptides are absorbed faster than proteins and amino acids. Therefore, oligopeptides have higher biological potency and nutrition...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K1/12C07K1/14C07K4/00
Inventor 杨瑞金许时婴王璋周凤娟
Owner JIANGNAN UNIV
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