Cell culture solution and NK cell culture method
A technology of cell culture and culture method, applied in the field of cell culture medium and NK cell culture, can solve the problems of low biological activity and poor expansion effect of NK cells, and achieve the effect of promoting rapid proliferation, simple operation and high biological activity.
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Embodiment 1
[0027] 1. Preparation of Peripheral Blood Mononuclear Cells
[0028] (1) Take 30 mL of peripheral blood, after anticoagulant treatment, add normal saline at a ratio of 1:1 between peripheral blood and normal saline, and mix well;
[0029] (2) Take another 50mL centrifuge tube, add 12.5mL of lymphocyte separation medium, and slowly transfer the diluted blood to the surface of the lymphocyte separation medium to form a clear interface between the two (blood dilution: lymphocyte separation medium =2:1), centrifuged at 800g for 15min;
[0030] (3) Take the liquid containing the peripheral blood mononuclear cell (PBMC) layer in the upper layer, be careful not to suck out the red blood cell layer, and transfer the crude peripheral blood mononuclear cell extract to another 50mL centrifuge tube;
[0031] (4) Resuspend the crude extract of peripheral blood mononuclear cells with 40mL RPMI 1640 medium, centrifuge at 500g for 5min, discard the supernatant, then resuspend the peripheral ...
Embodiment 2
[0038] 1. NK cell source and grouping
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