Probiotic for improving intestinal health and method for preparing the same

Abstract

This invention discloses a probiotic for improving gut health and its preparation method, belonging to the field of biopharmaceutical technology. The probiotic for improving gut health comprises the following raw materials in parts by weight: 20 parts of traditional Chinese medicinal herbs, 10 parts of liquid bacterial culture, 60 parts of culture medium, and the remainder being glucose. The liquid bacterial culture includes one or a combination of two of Bifidobacterium and Lactobacillus. The culture medium includes peptone, yeast extract, gelatin, and an acid-base buffer. In this invention, by adding traditional Chinese medicinal herbs to the raw materials, the probiotic achieves the therapeutic effects of traditional Chinese medicine on the basis of its traditional efficacy, thereby effectively improving gut health. Furthermore, the traditional Chinese medicinal herbs, including kudzu root, poria cocos, atractylodes lancea, licorice root, and cinnamon, all have good gut health-improving effects.

Description

Technical Field

[0001] This invention belongs to the field of biopharmaceutical technology, specifically relating to a probiotic for improving gut health and its preparation method. Background Technology

[0002] Probiotics are a class of live microorganisms that are beneficial to human health. When ingested in sufficient quantities, they can bring health benefits to the host. Probiotics are mainly found in the human gut, mouth, and vagina, playing an important role in maintaining the balance of the human gut microbiota, promoting nutrient absorption, enhancing immune function, and preventing disease. Characteristics of probiotics: Beneficial to health: They can improve the balance of gut microbiota, inhibit the growth of harmful bacteria, and enhance the body's immunity. Safe: They must be rigorously screened and identified to be harmless to humans. Stable: They should maintain their activity during storage, processing, and passage through the gastrointestinal tract.

[0003] Existing probiotics are mainly prepared from various bacterial strains, which have relatively few nutritional components and few medical effects. Therefore, this application proposes a probiotic that can improve intestinal health. Summary of the Invention

[0004] The technical problem to be solved by the present invention is to overcome the shortcomings of the prior art and provide a probiotic for improving intestinal health and a method for preparing the same.

[0005] The technical solution adopted to solve the above-mentioned technical problems is: a probiotic for improving intestinal health, comprising the following raw materials in parts by weight: The mixture consists of 20 parts of Chinese medicinal herbs, 10 parts of liquid bacterial culture, 60 parts of culture medium, and the remainder is glucose. The liquid bacterial culture includes one or a combination of two of Bifidobacterium and Lactobacillus. The culture medium includes peptone, yeast extract, gelatin, and acid-base buffer.

[0006] Furthermore, the Chinese medicinal materials include the following raw materials in parts by weight: 30 parts kudzu root, 15 parts poria cocos, 0.5 parts atractylodes lancea, 2 parts licorice root, and 0.5 parts cinnamon.

[0007] Furthermore, the acid-base buffer includes one of sodium citrate buffer and sodium acetate buffer.

[0008] Furthermore, the proportions of each raw material in the culture medium, by weight, are as follows: Peptone: Yeast extract: Gelatin: Acid-base buffer = 1.3:2:0.15:3.

[0009] A method for preparing probiotics for improving gut health, comprising: Step 1: Pre-treatment of Chinese medicinal materials: Select 30 parts of kudzu root, 15 parts of poria cocos, 0.5 parts of atractylodes lancea, 2 parts of licorice root and 0.5 parts of cinnamon, and put each Chinese medicinal material into a pulverizing device. The pulverizing device will pulverize each Chinese medicinal material into granules. Step 2, Extraction of Chinese medicinal materials: The Chinese medicinal materials obtained after the above steps are put into the extraction equipment, and the extraction equipment is used to boil the Chinese medicinal materials so that the medicinal components in the Chinese medicinal materials enter the extraction equipment to obtain Chinese medicine liquid. Step 3, Culture medium preparation: Select peptone, yeast extract, gelatin and acid-base buffer, place them in a petri dish, and then add an appropriate amount of water to the petri dish to prepare the culture medium; Step 4: Sterilization of the culture medium: The prepared culture medium is put into the sterilization equipment and sterilized by the sterilization equipment. Step 5, inoculation: Inoculate the liquid culture medium into the sterilized culture medium, then place the culture medium in the fermentation equipment and ferment for 50-60 hours to obtain probiotics; Step 6: Mixing: Mix the Chinese medicine liquid with the probiotics obtained in the above steps, and then place it in an environment of 35℃-37.5℃ for 2-3 hours to finally obtain probiotics containing Chinese medicine ingredients.

[0010] Furthermore, in step 2, during the decoction process of the Chinese medicinal materials, the extraction equipment separates the dregs and liquid from the raw materials after decoction. Then, the liquid is centrifuged and allowed to stand for at least 4 hours. Finally, the upper liquid of the centrifuged and settled liquid is removed using a tool to obtain a Chinese medicinal liquid with fewer impurities.

[0011] Furthermore, in step 4, high-temperature steam is introduced into the sterilization equipment at a temperature of 125℃-130℃ to inactivate bacteria in the culture medium. The sterilization time for high-temperature steam sterilization is set to 30 minutes.

[0012] Furthermore, in step 5, the temperature inside the fermentation equipment is maintained at 36-37℃ during fermentation.

[0013] Furthermore, in step 6, when the probiotics and the traditional Chinese medicine liquid are mixed, the traditional Chinese medicine liquid is first put into the mixing equipment, and then nitrogen gas is introduced into the mixing equipment so that the nitrogen gas will expel the oxygen in the mixing equipment. At the same time, the oxygen concentration in the mixing equipment is monitored in real time, and the nitrogen gas is stopped when the oxygen concentration reaches less than 1%.

[0014] Furthermore, in step 6, after obtaining the probiotic containing traditional Chinese medicine ingredients, the obtained probiotic containing traditional Chinese medicine ingredients is placed in a freeze-drying device for freeze-drying, and after drying, a powder of probiotic containing traditional Chinese medicine ingredients is obtained.

[0015] The beneficial effects of this invention are as follows: (1) In this invention, by adding Chinese medicinal materials to the raw materials, the probiotics can achieve the therapeutic effect of Chinese medicine on the basis of traditional efficacy, thereby effectively improving intestinal health. In addition, the Chinese medicinal materials such as kudzu root, poria cocos, atractylodes lancea, licorice and cinnamon have good intestinal improvement effects. (2) In this invention, when preparing probiotics, the traditional Chinese medicine liquid and probiotics are mixed by standing, so that the effective components of the probiotics and the traditional Chinese medicine liquid can be fully mixed, thereby increasing the content of the effective components of the traditional Chinese medicine liquid in the probiotics and improving the effect of probiotics in assisting the treatment of the intestinal environment. (3) In this invention, high-temperature steam is used to inactivate bacteria in the culture medium to avoid mixing with probiotics. In addition, when the probiotics and the traditional Chinese medicine liquid are mixed, nitrogen gas is continuously introduced into the mixing equipment until the oxygen content in the mixing equipment reaches a low-oxygen environment. This allows the probiotics to mix with the traditional Chinese medicine liquid in a low-oxygen environment, which is conducive to the rapid proliferation of probiotics in a low-oxygen environment and the expansion of the number of bacteria. Attached Figure Description

[0016] Figure 1 This is a schematic flowchart of a method for preparing probiotics for improving gut health according to the present invention. Figure 2 This is a schematic diagram of the method flow for step 2 in an embodiment of the present invention; Figure 3 This is a schematic diagram of the method flow for step 4 in an embodiment of the present invention; Figure 4 This is a schematic diagram of the method flow for step 6 in an embodiment of the present invention. Detailed Implementation

[0017] To make the objectives, technical solutions, and advantages of this invention clearer, the invention will be further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative and not intended to limit the invention.

[0018] This embodiment provides a probiotic for improving gut health, comprising the following raw materials by weight: 20 parts of traditional Chinese medicinal herbs, 10 parts of liquid bacterial culture, 60 parts of culture medium, with the remainder being glucose. The liquid bacterial culture includes one or a combination of two of Bifidobacterium and Lactobacillus. The culture medium includes peptone, yeast extract, gelatin, and an acid-base buffer. The traditional Chinese medicinal herbs include the following raw materials by weight: 30 parts of kudzu root, 15 parts of Poria cocos, 0.5 parts of Atractylodes lancea, 2 parts of licorice root, and 0.5 parts of cinnamon. The acid-base buffer includes one of sodium citrate buffer and sodium acetate buffer. The weight ratio of each raw material in the culture medium is: peptone: yeast extract: gelatin: acid-base buffer = 1.3:2:0.15:3.

[0019] like Figures 1-4 As shown, this embodiment also discloses a method for preparing probiotics for improving gut health, including: Step 1: Pre-treatment of Chinese medicinal materials: Select 30 parts of kudzu root, 15 parts of poria cocos, 0.5 parts of atractylodes lancea, 2 parts of licorice root and 0.5 parts of cinnamon, and put each Chinese medicinal material into a pulverizing device. The pulverizing device will pulverize each Chinese medicinal material into granules. Step 2, Extraction of Chinese medicinal materials: The Chinese medicinal materials obtained after the above steps are put into the extraction equipment, and the extraction equipment is used to boil the Chinese medicinal materials so that the medicinal components in the Chinese medicinal materials enter the extraction equipment to obtain Chinese medicine liquid. Specifically, such as Figure 2 As shown, step 2 includes the following steps: Step 2.1, Decoction: Put the Chinese medicinal materials into the extraction equipment for decoction; Step 2.2, Separation: After decoction, separate the dregs and liquid of the Chinese medicinal materials; Step 2.3, Centrifugation and Settling: Centrifuge the medicinal liquid after the above steps. After centrifugation, let it stand for at least 4 hours. Then, use a tool to remove the upper layer of liquid from the centrifuged and settled medicinal liquid to obtain a traditional Chinese medicine liquid with fewer impurities.

[0020] Step 3, Culture medium preparation: Select peptone, yeast extract, gelatin and acid-base buffer, place them in a petri dish, and then add an appropriate amount of water to the petri dish to prepare the culture medium; Step 4: Sterilization of the culture medium: The prepared culture medium is put into the sterilization equipment and sterilized by the sterilization equipment. Specifically, such as Figure 3 As shown, step 4 includes the following steps: Step 4.1: Introduce high-temperature steam: Continuously introduce high-temperature steam into the sterilization equipment. The temperature of the high-temperature steam is set to 125℃-130℃. Step 4.2, High-temperature sterilization: Put the culture medium prepared in step 3 into the sterilization equipment, and then use high-temperature steam to sterilize the culture medium, thereby inactivating the bacteria at high temperature. The sterilization time of high-temperature steam sterilization is set to 30 minutes. Step 5, inoculation: Inoculate the liquid culture medium into the sterilized culture medium, then place the culture medium in the fermentation equipment and ferment for 50-60 hours to obtain probiotics. Specifically, the temperature inside the fermentation equipment should be maintained at 36-37℃ during fermentation. Step 6: Mixing: Mix the Chinese medicine liquid with the probiotics obtained in the above steps, and then place it in an environment of 35℃-37.5℃ for 2-3 hours to finally obtain probiotics containing Chinese medicine ingredients.

[0021] Specifically, such as Figure 4 As shown, step 6 includes the following steps:

[0022] Step 6.1, Adding the Chinese medicine liquid: Add the Chinese medicine liquid into the mixing equipment, and then introduce nitrogen gas into the mixing equipment so that the nitrogen gas will expel the oxygen in the mixing equipment; Step 6.2 Monitoring oxygen concentration: When nitrogen is continuously introduced into the mixing equipment, the oxygen in the mixing equipment will be discharged, thereby reducing the oxygen concentration. At the same time, the oxygen concentration in the mixing equipment is monitored in real time. When the oxygen concentration reaches less than 1%, the nitrogen supply can be stopped. Step 6.3, Add probiotics: Add the probiotics to the mixing equipment with a low-oxygen environment inside, so that the probiotics can be mixed in an anaerobic environment, which is conducive to the proliferation of probiotics. At the same time, set the internal temperature of the mixing equipment to 35℃-37.5℃ and let it stand for 2-3 hours. Step 6.4, freeze drying: The probiotics containing traditional Chinese medicine ingredients obtained in the above steps are put into a freeze drying device for freeze drying. After drying, the probiotic powder containing traditional Chinese medicine ingredients is obtained.

[0023] The above are merely preferred embodiments of the present invention and are not intended to limit the scope of protection of the present invention.

Claims

1. A probiotic for improving gut health, characterized in that, Includes the following raw materials in parts by weight: The mixture consists of 20 parts of Chinese medicinal herbs, 10 parts of liquid bacterial culture, 60 parts of culture medium, and the remainder is glucose. The liquid bacterial culture includes one or a combination of two of Bifidobacterium and Lactobacillus. The culture medium includes peptone, yeast extract, gelatin, and acid-base buffer.

2. The probiotic for improving gut health according to claim 1, characterized in that, The Chinese medicinal materials include the following raw materials in parts by weight: 30 parts kudzu root, 15 parts poria cocos, 0.5 parts atractylodes lancea, 2 parts licorice root, and 0.5 parts cinnamon.

3. The probiotic for improving gut health according to claim 1, characterized in that, The acid-base buffer includes one of sodium citrate buffer and sodium acetate buffer.

4. The probiotic for improving gut health according to claim 1, characterized in that, The proportions of each raw material in the culture medium, by weight, are as follows: Peptone: Yeast extract: Gelatin: Acid-base buffer = 1.3:2:0.15:

3.

5. A method for preparing probiotics for improving gut health, characterized in that, include: Step 1: Pre-treatment of Chinese medicinal materials: Select 30 parts of kudzu root, 15 parts of poria cocos, 0.5 parts of atractylodes lancea, 2 parts of licorice root and 0.5 parts of cinnamon, and put each Chinese medicinal material into a pulverizing device. The pulverizing device will pulverize each Chinese medicinal material into granules. Step 2, Extraction of Chinese medicinal materials: The Chinese medicinal materials obtained after the above steps are put into the extraction equipment, and the extraction equipment is used to boil the Chinese medicinal materials so that the medicinal components in the Chinese medicinal materials enter the extraction equipment to obtain Chinese medicine liquid. Step 3, Culture medium preparation: Select peptone, yeast extract, gelatin and acid-base buffer, place them in a petri dish, and then add an appropriate amount of water to the petri dish to prepare the culture medium; Step 4: Sterilization of the culture medium: The prepared culture medium is put into the sterilization equipment and sterilized by the sterilization equipment. Step 5, inoculation: Inoculate the liquid culture medium into the sterilized culture medium, then place the culture medium in the fermentation equipment and ferment for 50-60 hours to obtain probiotics; Step 6: Mixing: Mix the Chinese medicine liquid with the probiotics obtained in the above steps, and then place it in an environment of 35℃-37.5℃ for 2-3 hours to finally obtain probiotics containing Chinese medicine ingredients.

6. The method for preparing probiotics for improving gut health according to claim 5, characterized in that, In step 2, during the decoction process of the Chinese medicinal materials, the extraction equipment separates the dregs and liquid from the raw materials after decoction. Then, the liquid is centrifuged and allowed to stand for at least 4 hours. Finally, the upper liquid of the centrifuged liquid is removed using a tool to obtain a Chinese medicinal liquid with fewer impurities.

7. The method for preparing probiotics for improving gut health according to claim 5, characterized in that, In step 4, high-temperature steam is introduced into the sterilization equipment. The temperature of the high-temperature steam is 125℃-130℃. The bacteria in the culture medium are inactivated by steam using the high-temperature steam. In addition, the sterilization time of the high-temperature steam sterilization is set to 30 minutes.

8. The method for preparing probiotics for improving gut health according to claim 5, characterized in that, In step 5, the temperature inside the fermentation equipment is maintained at 36-37℃ during fermentation.

9. The method for preparing probiotics for improving gut health according to claim 5, characterized in that, In step 6, when the probiotics and the traditional Chinese medicine liquid are mixed, the traditional Chinese medicine liquid is first put into the mixing equipment, and then nitrogen gas is introduced into the mixing equipment so that the nitrogen gas will expel the oxygen in the mixing equipment. At the same time, the oxygen concentration in the mixing equipment is monitored in real time, and the nitrogen gas is stopped when the oxygen concentration reaches less than 1%.

10. The method for preparing probiotics for improving gut health according to claim 5, characterized in that, In step 6, after obtaining probiotics containing traditional Chinese medicine ingredients, the obtained probiotics containing traditional Chinese medicine ingredients are placed in a freeze-drying device for freeze-drying, and then the powder of probiotics containing traditional Chinese medicine ingredients is obtained after drying.

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