Cell differntiation inhibiting agent, cell culture method using the same, culture medium, and cultured cell line

a cell differentiation and inhibiting agent technology, applied in the field of stem cell differentiation inhibiting agents, can solve the problems of complex preparation of mouse primary fibroblasts, insufficient donor numbers, and poor quality of primary fibroblasts
US20050153941A1Inactive Publication Date: 2005-07-14ASAHI KASEI KK
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Patent Information

Authority / Receiving Office
US · United States
Patent Type
Applications(United States)
Current Assignee / Owner
ASAHI KASEI KK
Publication Date
2005-07-14
Estimated Expiration
Not applicable · inactive patent

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Abstract

The object of the present invention is to provide a differentiation inhibiting agent which allows culture of a stem cell or an embryonic stem cell in an undifferentiated state without use of any feeder cell, a method for culturing using the same, a cell culture liquid using the same, and a cell prepared by culturing using this differentiation inhibiting agent. The present invention provides a differentiation inhibiting agent which comprises a low molecular weight compound, especially a tetrahydroisoquinoline derivative, as an active ingredient; a method for safely culturing a stem cell in large scale in undifferentiated state in the absence of feeder cell which comprises culturing a stem cell by using a tetrahydroisoquinoline derivative; a culture liquid for stem cells comprising a tetrahydroisoquinoline derivative; and a cell which is obtained by culture using a tetrahydroisoquinoline derivative as a differentiation inhibiting agent.
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Description

TECHNICAL FIELD

[0001] The present invention relates to a stem cell differentiation inhibiting agent comprising a low molecular weight compound, especially a tetrahydroisoquinoline derivative, as an active ingredient, a stem cell culture method by using the same, a culture medium, and a cultured stem cell line prepared by using the same. The present invention relates further to new bicyclic compounds having an action of maintaining an undifferentiated state of the stem cell. BACKGROUND ART

[0002] An organ and a tissue damaged by an injury, a disease or aging require promotion of regeneration to recover its function. Particularly, a substantial organ such as heart, liver, kidney, or pancreas is essential for maintaining life, and thus hypofunction and a function thereof causes a death. Therefore transplantation treatment such as organ transplantation is popularly conducted intending life saving. However, the number of donor is generally insufficient, and thus a new approach to solve ...

Claims

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