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Stem Cells Culture Systems

a technology of stem cells and culture systems, applied in the field of stem cells, can solve the problems of preventing the propagation of pure populations of undifferentiated stem cells, affecting the differentiation of somatic differentiation cells, and a low efficiency, so as to inhibit or prevent the differentiation of stem cells, inhibit or prevent the effect of sc differentiation

Inactive Publication Date: 2009-04-23
HADASIT MEDICAL RES SERVICES & DEVMENT
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Benefits of technology

[0032]In accordance with this second aspect, there is also provided a method for inhibiting or preventing differentiation of stem cells to extraembryonic cells, the method comprises incubating said stem cells in a culture system comprising NA or a derivative of NA having an inhibitory effect on differentiation of stem cells to extraembryonic cell

Problems solved by technology

It was found that in this system the cells are propagated as clumps on a low scale which does not allow cloning2.
This system allows cloning of undifferentiated hESC, although at a low efficiency3.
A key limitation of hESC culture systems is that they do not allow the propagation of pure populations of undifferentiated stem cells and their use always involves some level of background differentiation.
Spontaneous differentiation of hESC into presumably extraembryonic lineages also interferes with the derivation of somatic differentiated cells.

Method used

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Embodiment Construction

[0075]The invention is described in the following detailed description with reference to cell cultures and culture systems for handling stem cells, preferably human embryonic stem cells. It should be noted that in addition to the cell cultures and culture systems discussed in detailed hereinbelow, also encompassed within the present invention are uses of specific components described with reference to the culture system in the preparation of such culture systems as well as to methods of use of the culture system in handling stem cells cultures and methods of preparing culture cells.

[0076]As used in the specification and claims, the forms “a”, “an” and “the” is include singular as well as plural references unless the context clearly dictates otherwise. For example, the term “a culture system” includes one or more culture systems.

[0077]As used herein, the term “or” means one or a combination of two or more of the listed choices

[0078]Further, as used herein, the term “comprising” is in...

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Abstract

The present invention concerns systems and methods for providing human cell cultures. Specific embodiments of the invention relate to cultures of feeder cells for use in stem cell technology, as well as cultures, culture systems and methods for maintenance and propagating of stem cells in an undifferentiated state as well as for the development of somatic cells cultures from stem cells, the somatic cell cultures being free of extraembryonic cells.

Description

FIELD OF THE INVENTION[0001]The invention relates to stem cells (SC) in particularly to methods and systems for handling human embryonic stem cells (hESC).LIST OF PRIOR ART[0002]The following is a list of prior art, which is considered to be pertinent for describing the state of the art in the field of the invention.[0003](1) Thomson, J. A. et al. Embryonic stem cell lines derived from human blastocysts. Science 282, 1145-1147 (1998).[0004](2) Reubinoff, B. E., Pera, M. F., Fong, C. Y., Trounson, A. & Bongso, A. Embryonic stem cell lines from human blastocysts: somatic differentiation in vitro. Nat Biotechnol 18, 399-404 (2000)[0005](3) Amit, M. et. al. Clonally derived human embryonic stem cell lines maintain pluripotency and proliferative potential for prolonged periods of culture. Dev Biol 227, 271-278 (2000).[0006](4) Xu, C. et al. Feeder-free growth of undifferentiated human embryonic stem cells. Nat Biotechnol 19, 971-974 (2001).[0007](5) Amit, M. et al. Human feeder layers fo...

Claims

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Application Information

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IPC IPC(8): C12N5/02C12N5/08C12N5/071C12N5/0735
CPCC12N2501/16C12N5/0621C12N2500/38C12N2500/99C12N2506/02C12N2501/115C12N2500/44C12N2502/13C12N2501/155C12N2500/32C12N5/0606C12N2533/52C12N2500/90C12N2500/98
Inventor SHUSHAN, ETTI BENTANNENBAUM, SHELLYITSYKSON, PAVELBANIN, EYALREUBINOFF, BENJAMIN
Owner HADASIT MEDICAL RES SERVICES & DEVMENT
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