Thin-layer chromatography identification method of carbon traditional Chinese medicine formula granules
A technology of traditional Chinese medicine formula granules and thin-layer chromatography, which is applied in the field of TLC identification of carbon-based traditional Chinese medicine formula granules, can solve problems such as lack of quality control methods, and achieve the effects of comprehensive detection results, simple operation, and strong specificity
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experiment example 1
[0055] Experimental example 1 TLC identification of madder charcoal formula granules
[0056] Take 0.5g of madder charcoal formula granules, put them in a Erlenmeyer flask, add 15ml of water for ultrasonic dissolution, add diethyl ether for extraction twice, 20ml each time, discard the ether layer, add 5ml of 15% hydrochloric acid to the water layer, and heat in a water bath at 98°C for 60 minutes. Allow to cool and filter; the filtrate is extracted twice with ethyl acetate, 20ml each time, the ethyl acetate solution is combined, evaporated to dryness, and the residue is dissolved in 1ml of methanol as the test solution. Take another 2.0 g of Rubia reference drug, add 80 ml of water, boil for 30 minutes, filter, concentrate the filtrate to 15 ml, add ether to extract twice, and prepare the rest as the test sample to make a reference drug solution. According to the thin-layer chromatography ("Chinese Pharmacopoeia" 2010 edition, an appendix VIB) test, absorb 5 μl each of the ...
experiment example 2
[0057] TLC Identification of Experimental Example 2 Maogen Charcoal Formula Granules
[0058] Take 1.0g of Chine charcoal formula granules, put them in a conical flask, add 15ml of water to dissolve them ultrasonically, add 5ml of 15% hydrochloric acid, heat and reflux at 110°C for 45 minutes, let cool, and filter; the filtrate is extracted twice with ethyl acetate, 20ml each time, combined ethyl acetate solution, evaporated to dryness; add 1ml methanol to the residue to dissolve, as the test solution. Take another 1.0g of Imperata rhizome reference medicinal material, add 80ml of water, boil for 30 minutes, filter, concentrate the filtrate to 15ml, add 5ml of 15% hydrochloric acid, and prepare the rest with the same preparation method of the test sample as the reference medicinal solution. According to the thin-layer chromatography ("Chinese Pharmacopoeia" 2010 edition, an appendix VIB) test, absorb 5 μl each of the test solution and the control medicinal solution, and spot...
experiment example 3
[0059] TLC Identification of Experimental Example 3 Thuja Charcoal Formula Granules
[0060] Take 1.0g of Arborvitae charcoal formula granules, put them in a conical flask, add 25ml of water to dissolve them ultrasonically, add 3ml of 15% hydrochloric acid, heat in a water bath at 100°C for 30 minutes, let cool, and filter; the filtrate is extracted twice with ethyl acetate, Each 25ml, combined ethyl acetate solution, evaporated to dryness, the residue was dissolved in 1ml of methanol, as the test solution. Take another 2.0g of Arborvitae leaves as reference medicinal material, add 80ml of water, boil for 30 minutes, filter, concentrate the filtrate to 25ml, add 3ml of 15% hydrochloric acid, and prepare the rest as the reference medicinal material solution by the same preparation method as the test sample. According to the thin-layer chromatography ("Chinese Pharmacopoeia" 2010 edition, an appendix VIB) test, draw 5 μl of each of the above two solutions, and respectively spo...
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