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Thin-layer chromatography identification method of carbonized herb processed product

A technique of thin-layer chromatography and processed products, applied in the field of quality testing of traditional Chinese medicines, can solve the problems of different processing degrees, uneven quality of charcoal decoction pieces, lack of identification items of charcoal medicine pieces, etc., and achieves strong specificity and comprehensive testing results. , identify the effect of strong specificity

Inactive Publication Date: 2016-01-13
GUANGDONG YIFANG PHARMA
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  • Summary
  • Abstract
  • Description
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AI Technical Summary

Problems solved by technology

The quality of decoction pieces of charcoal medicine in the market is uneven, and the degree of processing is different, and the Chinese Pharmacopoeia lacks identification items for decoction pieces of charcoal medicine, which greatly restricts the use of decoction pieces of charcoal medicine; therefore, it is necessary to study an objective, fast, simple and comprehensive method. Evaluation of quality control methods for processed charcoal products

Method used

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  • Thin-layer chromatography identification method of carbonized herb processed product
  • Thin-layer chromatography identification method of carbonized herb processed product
  • Thin-layer chromatography identification method of carbonized herb processed product

Examples

Experimental program
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Effect test

experiment example 1

[0051] Experimental example 1 Thin-layer identification of orientalis charcoal

[0052] Take 4.0g of orientalis charcoal powder, put it in a conical flask, add 30ml of 15% hydrochloric acid solution, heat it in a 98°C water bath for 60 minutes, let it cool, and filter; extract the filtrate twice with ether, 20ml each time, and combine ether solution, evaporated to dryness; add methanol 1ml to the residue to dissolve, as the test solution. In addition, 1.0 g of Arborvitae leaf reference medicinal material was taken, and the reference medicinal material solution was prepared with the preparation method of the test sample. According to the thin-layer chromatography ("Chinese Pharmacopoeia" 2010 edition, an appendix VIB) test, absorb 5 μl each of the test solution and the control medicinal solution, and spot on the same silica gel GF respectively. 254 On the TLC plate, use toluene-ethyl formate-formic acid (5:5:0.5) as the developer, develop, take it out, dry it, and inspect it...

experiment example 2

[0053] TLC identification of experimental example 2 Burnet charcoal

[0054] Take 3.0g of Burnet charcoal powder, put it in a conical flask, add 30ml of 20% hydrochloric acid solution, heat and reflux at 110°C for 45 minutes, let cool, and filter; the filtrate is extracted twice with ether, 20ml each time, and the ether solution is combined, Evaporate to dryness; add methanol 1ml to the residue to dissolve, as the test solution. Another Burnet reference medicinal material 1.0g, the same preparation method as the test sample to prepare the reference medicinal solution. According to the thin-layer chromatography ("Chinese Pharmacopoeia" 2010 edition, an appendix VIB) test, absorb 5 μl each of the test solution and the control medicinal solution, and spot on the same silica gel GF respectively. 254 On the TLC plate, use toluene-ethyl formate-formic acid (5:5:0.5) as the developer, develop, take it out, dry it, and inspect it under an ultraviolet lamp (254nm); Figure 3-4 , in...

experiment example 3

[0055] TLC identification of experimental example 3 vinegar moxa charcoal

[0056] Take 4.0g of vinegar moxa powder, put it in a conical flask, add 30ml of 15% hydrochloric acid solution, heat it in a water bath at 100°C for 60 minutes, let it cool, and filter; extract the filtrate twice with ether, 20ml each time, and combine ether solution, evaporated to dryness; add methanol 1ml to the residue to dissolve, as the test solution. Another 1.0 g of Artemisia argyi reference drug was taken, and the reference drug solution was prepared with the same preparation method as the test sample. According to the thin-layer chromatography ("Chinese Pharmacopoeia" 2010 edition, an appendix VIB) test, absorb 5 μl each of the test solution and the control medicinal solution, and spot on the same silica gel GF respectively. 254 On the TLC plate, use toluene-ethyl formate-formic acid (5:5:0.5) as the developer, develop, take it out, dry it, and inspect it under an ultraviolet lamp (254nm); ...

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Abstract

The invention discloses a thin-layer chromatography identification method of a carbonized herb processed product. The method comprises the steps that 1, sample powder is taken, hydrochloric acid is added, heating is conducted for hydrolysis, filtration is conducted, filtrate is extracted through ethyl acetate or ethyl ether, extract liquor is evaporated to dryness, and residues are dissolved through methyl alcohol to serve as a test sample solution; 2, reference medicinal materials are taken, and then a reference medicinal material solution is prepared through the method for preparing a test sample; 3, the test sample solution and the reference medicinal material solution are sucked and dripped on a same silica gel GF254 thin-layer plate, developing is conducted by taking methylbenzene, ethyl formate and formic acid as developing solvents according to the ratio of 2-8 to 2-8 to 0.1-1, and the thin-layer plate is taken out and dried in the air; 4, the thin-layer plate is viewed under a 254 nm ultraviolet lamp, and principal spots with the same color are showed on the positions, corresponding to the chromatography of the reference medicinal materials, in the test sample chromatography. The identification method is rapid, comprehensive and high in specificity, the requirements of the carbonizing drug characteristic of the carbonized herb product can be embodied, and a certain reference basis is provided for establishment of quality standards of the carbonized herb products.

Description

technical field [0001] The invention belongs to the technical field of traditional Chinese medicine quality detection, and in particular relates to a thin-layer chromatography identification method for processed charcoal medicine. Background technique [0002] The use of traditional Chinese charcoal medicine has a history of more than two thousand years. It is a processed product of traditional Chinese medicine with wide application and definite curative effect. Up to now, dozens of kinds are still commonly used in clinic, especially in the treatment of hemorrhagic diseases, such as Blood residual charcoal, large thistle charcoal, small thistle charcoal, burnet charcoal, madder charcoal, puhuang charcoal, etc. [0003] The purpose of making charcoal from traditional Chinese medicine is to achieve: change the inherent properties of the medicine; enhance the effect of astringent and hemostasis, astringent and antidiarrheal; ease and reduce the efficacy of drug toxicity. Durin...

Claims

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Application Information

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IPC IPC(8): G01N30/90
Inventor 谭登平程学仁朱德全林振东刘丽萍王闽予陈向东
Owner GUANGDONG YIFANG PHARMA
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