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Flavonoid glycoside compound in flos elaeagni angustifoliae, and preparation method thereof

A technology of middle flavonoid glycosides and anthocyanidin, which is applied in the preparation of sugar derivatives, chemical instruments and methods, sugar derivatives, etc., can solve the problems of seldom research on monomer components of jujube flower.

Active Publication Date: 2018-06-29
XINJIANG TECHN INST OF PHYSICS & CHEM CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the domestic research on the chemical constituents of Rhododendron is mainly focused on the study of volatile oil components and the enrichment and content determination of flavonoids, and few studies focus on the monomer components of Rhizoma Rhizoma.

Method used

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  • Flavonoid glycoside compound in flos elaeagni angustifoliae, and preparation method thereof
  • Flavonoid glycoside compound in flos elaeagni angustifoliae, and preparation method thereof
  • Flavonoid glycoside compound in flos elaeagni angustifoliae, and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] a. Take 1 kg of dried whole flowers of Elaeagnus eraeae, after pulverization, use 5 times the volume concentration of 95% methanol aqueous solution to extract once at room temperature, combine the filtrates, evaporate to dryness, and obtain the extract;

[0024] b. Disperse the extract obtained in step a with water, extract 3 times with petroleum ether, collect the extract, evaporate the solvent in vacuo to obtain petroleum ether extract extract; use ethyl acetate to extract the remaining water layer Extract 3 times, collect the ethyl acetate extract, combine and dry to obtain the ethyl acetate extract extract; extract the remaining water layer with n-butanol for 3 times, collect the n-butanol extract, combine and dry , to obtain n-butanol extract extract;

[0025] c. The n-butanol extract extract obtained in step b is separated by MCI column chromatography, and gradient elution is carried out with methanol aqueous solution with a volume ratio of 10%-100%, and the volum...

Embodiment 2

[0027] a. Take 1 kg of dried whole flowers of Elaeagnus eraeae, after pulverization, use 5 times the volume concentration of 95% ethanol aqueous solution to extract twice at room temperature, combine the filtrates, evaporate to dryness, and obtain the extract;

[0028] b. Disperse the extract obtained in step a with water, extract 4 times with petroleum ether, collect the extract, evaporate the solvent in vacuo to obtain petroleum ether extract extract; use ethyl acetate to extract the remaining water layer Extract 4 times, collect the ethyl acetate extract, combine and dry to obtain the ethyl acetate extract extract; extract the remaining water layer with n-butanol for 4 times, collect the n-butanol extract, combine and dry , to obtain n-butanol extract extract;

[0029] c. The n-butanol extract extract obtained in step b is separated by MCI column chromatography, and gradient elution is carried out with methanol aqueous solution with a volume ratio of 10%-100%, and the volum...

Embodiment 3

[0031] a. Take 1 kg of dried whole flowers of Elaeagnus eraeae, after pulverization, use 10 times volume concentration of 95% methanol aqueous solution at a temperature of 70°C to extract by heating and refluxing for 3 times, and evaporate the extract to dryness to obtain an extract;

[0032] b. Disperse the extract obtained in step a with water, extract 5 times with petroleum ether, collect the extract, evaporate the solvent in vacuo to obtain petroleum ether extract extract; use ethyl acetate to extract the remaining water layer Extract 5 times, collect the ethyl acetate extract, combine and dry to obtain the ethyl acetate extract extract; extract the remaining water layer with n-butanol for 5 times, collect the n-butanol extract, combine and dry , to obtain n-butanol extract extract;

[0033] c. The n-butanol extract extract obtained in step b is separated by MCI column chromatography, and gradient elution is carried out with methanol aqueous solution with a volume ratio of...

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Abstract

The invention relates to a flavonoid glycoside compound in flos elaeagni angustifoliae, and a preparation method thereof. The method comprises the following steps: taking flos elaeagni angustifoliae medicines as raw materials, extracting by solvent, extracting by solvent, carrying out separation through two or three ways including positive phase and reverse phase silica gel column chromatography,or glucan gel LH-20 column chromatography and a semi-preparation high-performance liquid chromatography, adopting thin-layer chromatography and the high-performance liquid chromatography to detect andanalyze to obtain two new flavonoid glycoside compounds, wherein the first compound is flos elaeagni angustifoliae glycoside A, and the second compound is flos elaeagni angustifoliae glycoside B. Through the measurement of in vitro inhibition of COX (cyclo-oxygense) enzyme activity, an experiment result indicates that the flavonoid glycoside compound separated from the flos elaeagni angustifoliaecan inhibits the COX enzyme activity in different degrees, and the flavonoid glycoside compound in flos elaeagni angustifoliae can be used for preparing anti-inflammatory medicines.

Description

technical field [0001] The present invention belongs to the field of natural product chemistry, specifically to the field of extraction, separation and preparation of effective components of natural products, and in particular to the extraction and separation of two novel flavonoid glycosides with pharmacological activity from the medicinal material of Rhododendron flavonoides. Background technique [0002] Elaeagnus angustifolia L. is the flower of Elaeagnus angustifolia L. in the Elaeagnaceae family (Elaeagnaceae), which grows in semi-arid, arid, semi-desert, and desert regions. Osmanthus fragrans is known as "Osmanthus fragrans in the desert" because of its fragrance similar to sweet-scented osmanthus in the south of the Yangtze River. Eleuthero jujube is native to West Asia, and it is also distributed along the Mediterranean coast, India and the former Soviet Union. In my country, Elaeoptera is mainly distributed in the northwest provinces and western Inner Mongolia. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H1/08C07H17/07A61P29/00
CPCC07H1/08C07H17/07
Inventor 袁涛陈晓雨刘玉霜
Owner XINJIANG TECHN INST OF PHYSICS & CHEM CHINESE ACAD OF SCI
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