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Preparation method for zein

A technology for zein and corn gluten meal, which is applied in the field of preparation of zein, can solve problems such as hindering the application and popularization of zein, and achieve the effects of low cost, improved purity and low production cost

Inactive Publication Date: 2015-07-15
GUANGDONG FOOD IND INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

All of the above have hindered the application and promotion of zein in the market

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Take the weakly acidic macroporous resin D151, and pre-treat it before loading the column. The pre-treatment process is: first rinse with water, and then use 5% (mass percentage, the same below) NaOH and 5% (volume percentage, The same below) soak and rinse with HCl to remove residual organic solvents, low molecular polymers and organic impurities in the resin. After the resin was treated with 5% hydrochloric acid, it became H-type resin, and then packed into a column. After loading the column, add 2 times column volume of ethanol aqueous solution to equilibrate the column, wherein the volume percentage of ethanol in the ethanol aqueous solution is 70%.

[0029] Weigh 100 g of zein powder, add 800 ml of ethanol aqueous solution with a volume percentage of 70%, extract at 50°C for 5 hours, and filter to obtain zein extract; The flow rate is to add the H-type weakly acidic macroporous resin D151 adsorbed protein that has been balanced by 70% ethanol solution by volume, a...

Embodiment 2

[0036] Take the weakly acidic macroporous resin D152, and pre-treat it before loading into the column. The pre-treatment process is: first, rinse with water, and then soak and rinse with 5% NaOH and 5% HCl successively to remove residual organic solvents and low molecular weight in the resin. Polymers and organic impurities. After the resin was treated with 5% hydrochloric acid, it became H-type resin, and then packed into a column. After loading the column, add 3 times column volume of ethanol aqueous solution to equilibrate the column, wherein the volume percentage of ethanol in the ethanol aqueous solution is 71%.

[0037] Weigh 100 g of zein powder, add 1000 ml of ethanol solution with a volume percentage of 71%, extract at 50°C for 6 hours, filter to obtain zein extract, and distill the zein extract at 0.4 column volume per hour The flow rate is to add H-type weakly acidic macroporous resin D152 adsorbed protein that has been balanced by 71% ethanol solution, and then ad...

Embodiment 3

[0040]Take the weakly acidic macroporous resin DK110, which is pretreated before loading into the column. The pretreatment process is: first, rinse with water, and then soak and rinse with 5% NaOH and 5% HCl successively to remove the remaining organic solvents and low molecular weight in the resin. Polymers and organic impurities. After the resin was treated with 5% hydrochloric acid, it became H-type resin, and then packed into a column. After loading the column, add 5 times column volume of ethanol aqueous solution to equilibrate the column, wherein the volume percentage of ethanol in the ethanol aqueous solution is 75%.

[0041] Weigh 100 g of corn gluten powder, add 1000 ml of 75% ethanol solution by volume, extract at 60°C for 3 hours, and filter to obtain a zein extract. Add the extract at a flow rate of 0.5 times the column volume per hour to the H-type weakly acidic macroporous resin DK110 adsorbed protein that has been balanced by an ethanol solution with a volume p...

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PUM

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Abstract

The invention discloses a preparation method for zein. The preparation method comprises the following steps: with corn gluten meal as a raw material, adding ethanol water to extract the corn gluten meal; carrying out filtering and separating so as to obtain zein extract; adding the zein extract into a resin for chromatography; after removal of impurities, eluting protein with salt-containing ethanol water; and successively carrying out concentration, ethanol recovery, washing and drying so as to obtain a finished zein product. The method has the advantages of low cost and small influence on the environment; and the obtained finished zein product has a light color, low impurity content and high purity and is suitable for industrial production.

Description

technical field [0001] The invention belongs to the technical field of zein, and in particular relates to a preparation method of zein. Background technique [0002] Zein is the main protein in corn gluten meal. It is insoluble in water and anhydrous alcohols, soluble in 60~95% (V / V) alcohol aqueous solution, and also soluble in strong alkali, acetone, acetic acid and other organic solvents. The zein solution has film-forming properties. When the solvent evaporates, the protein aggregates to form a smooth, transparent zein film with the characteristics of moisture resistance, oxygen resistance, and enteric solubility. Due to the unique solubility and film-forming properties of zein, it has broad market prospects in the fields of medicine, hygiene and food chemical industry. It can be used as pharmaceutical excipients, tablet coating and sustained-release wall materials, and Used in candy coating, fresh-keeping coating for fruits and meat products, wall materials for oil em...

Claims

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Application Information

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IPC IPC(8): C07K14/425C07K1/36C07K1/34C07K1/16
CPCC07K14/425
Inventor 梁丽敏徐勇杜阳吉刘春凤卫娜袁娟
Owner GUANGDONG FOOD IND INST
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