Method for extraction of maize yellow pigment and alcohol soluble protein from maize protein powder

The technology of corn gluten meal and corn yellow pigment is applied in the field of corn deep processing, which can solve the problems of low extraction rate and purity of products, difficult to widely use, long extraction time, etc. Effect

Inactive Publication Date: 2015-03-11
SHAANXI UNIV OF SCI & TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Supercritical carbon dioxide extraction technology is an emerging extraction technology with high pollution-free efficiency, but its high cost is difficult to be widely used in industry
[0008] There are many studies on the extraction of zeaxanthin or gliadin from corn gluten alone, but there are few studies on the simultaneous extraction of the two substances
The simple use of solvent extraction has problems such as long extraction time and low extraction efficiency, and the extraction products are used in the food industry, which has high requirements for the safety of solvents
In the method currently proposed, a certain concentration of ethanol is used for one-step extraction, and the two substances of zeaxanthin and prolamin are obtained after optimizing the process conditions, but the extraction rate and purity of the obtained products are low, and the products need to be further separated.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] The corn gluten powder is crushed and passed through a 60-mesh sieve for later use. Add 95% ethanol according to the material-to-liquid ratio of 1:14, process at 300W ultrasonic power for 20 minutes, centrifuge at 3000r / min, centrifuge for 10 minutes, take the supernatant and carry out vacuum distillation at 50±5°C until it becomes orange-red oily The liquid is freeze-dried at -80°C for 24 hours to obtain the finished product of zeaxanthin. Collect the distilled ethanol and dilute to a concentration of 70% ethanol. After drying the centrifuged precipitate, add diluted 70% ethanol according to the ratio of 1:10 to liquid, treat it under 250W ultrasonic power for 50 minutes, adjust the isoelectric point pH=6.2, precipitate and collect prolamin, wash it repeatedly and then dry it in vacuum. Glamin finished product. Among them, the extraction rates of zeaxanthin and gliadin are 5.73% and 8.84%, respectively.

Embodiment 2

[0029] The corn gluten powder is crushed and passed through a 60-mesh sieve for later use. Add 95% ethanol according to the material-to-liquid ratio of 1:12, process for 30 minutes at 200W ultrasonic power, centrifuge at 2500r / min, centrifuge for 5 minutes, take the supernatant and carry out vacuum distillation at 50±5°C until it becomes orange-red oily The liquid is freeze-dried at -60°C for 20 hours to obtain the finished product of zeaxanthin. The distilled ethanol was collected and diluted to a concentration of 60% ethanol. After drying the centrifuged precipitate, add diluted 60% ethanol according to the ratio of solid to liquid 1:12, and treat it under 200W ultrasonic power for 50 minutes, adjust the isoelectric point, pH = 6.0, precipitate and collect prolamin, and vacuum dry it after repeated washing. Obtain gliadin products. Among them, the extraction rates of zeaxanthin and gliadin are 5.44% and 6.97%, respectively.

Embodiment 3

[0031] The corn gluten powder is crushed and passed through a 60-mesh sieve for later use. Add 95% ethanol at a material-to-liquid ratio of 1:16, process at 400W ultrasonic power for 10 minutes, centrifuge at 2500r / min, centrifuge for 15 minutes, take the supernatant and carry out vacuum distillation at 50±5°C until it becomes orange-red oily The liquid is freeze-dried at -70°C for 30 hours to obtain the finished product of zeaxanthin. The distilled ethanol was collected and diluted to a concentration of 80% ethanol. After drying the centrifuged precipitate, add diluted 80% ethanol according to the ratio of solid to liquid 1:8, treat it under 300W ultrasonic power for 40 minutes, adjust the isoelectric point, pH = 6.2, collect the precipitated prolamin, and vacuum dry it after repeated washing. Obtain gliadin products. Among them, the extraction rates of zeaxanthin and gliadin are 5.06% and 7.59%, respectively.

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Abstract

The invention discloses a method for extraction of maize yellow pigment and alcohol soluble protein from maize protein powder. The method includes: crushing the maize protein powder, then conducting sieving by a 60-100 mesh sieve, adding ethanol with a material-liquid ratio of 1:(8-16), conducting ultrasonic treatment at 200-600W for 10-50min by an ultrasound assisted extraction technique; centrifuging the raw material and the extraction solvent, then collecting the supernatant and performing reduced pressure distillation till an orange red oily liquid, and conducting drying to obtain a pigment product; drying the centrifuged precipitate, adding ethanol diluted to 60%-90% according to a material-liquid ratio of 1:(6-14), conducting ultrasonic treatment under ultrasonic power of 200-400W for 10-50min, performing centrifugation and taking the supernatant, adjusting isoelectric points, collecting alcohol soluble protein from the precipitate, and conducting drying to obtain the finished product. According to the invention, maize yellow pigment and alcohol soluble protein with high purity can be extracted in steps by one production process, the solvent can be recycled, the production cost is saved, the extraction efficiency is improved, and the operation is simple, so that the method is easy to realize industrialization.

Description

technical field [0001] The invention relates to the technical field of corn deep processing, in particular to a method for extracting zeaxanthin and gliadin from corn gluten powder. Background technique [0002] Corn gluten powder is the leftovers of corn starch produced by wet method, which is rich in zeaxanthin and gliadin. Zeaxanthin can be added to food as a natural colorant and has strong antioxidant properties; zeaxanthin is The main component of corn pigment is a carotenoid mixture mainly composed of β-carotene, cryptoxanthin, lutein and zeaxanthin, and belongs to isoprenoid pigments. Zeaxanthin can be used as a natural coloring agent in meat, eggs and other foods. According to the "Hygienic Standards for the Use of Food Additives" GB 2760-2011 formulated by my country, zeaxanthin can be used as a functional coloring agent and can be added to hydrogenated vegetable oil and candy In other foods, the maximum dosage is 5.0g / kg. [0003] The molecular structure of each c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09B61/00C07K14/425C07K1/14
CPCC07K14/425C09B61/00
Inventor 董文宾郎繁繁
Owner SHAANXI UNIV OF SCI & TECH
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