Method of separating and purifying phosphatidyl choline from phospholipid by resin chromatography method

A technology for separation and purification of phosphatidylcholine, applied in the direction of edible phospholipid composition, application, protein food ingredients, etc., can solve the problems of low cost, short elution cycle, long elution cycle, etc.

Active Publication Date: 2007-09-12
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] Phosphatidylcholine has important medicinal value, and the separation of phosphatidylcholine from phospholipids has always been an important subject of phospholipid research at home and abroad. At present, the separation methods studied by domestic and foreign scholars mainly include low-carbon alcohol method, ultrafiltration method, and precipitation method. , chromatography and supercritical fluid method, etc., among them, chromatography can be divided into the following types according to different fillers: aluminum oxide column chromatography, silica gel column chromatography, ion exchange column chromatography, the above methods are mainly It is aimed at the separation of two phospholipids, PC and PE, and the purity of the obtained PC product is about 90%. The aluminum oxide column has a strong adsorption capacity for other phospholipids and can only elute the more polar PC from the column. Down, the elution period is short but lysophospholipids will be produced in the process; the silica gel column can realize the separation of PC, PE, PI and other phospholipids, but the elution period is longer; the research on ion exchange resin separation of phospholipids is less, only The feasibility of resin separation of phospholipids has been studied, but the research on specific process parameters is not systematic enough
According to the structural characteristics of the isolate, a method for separating phosphatidylcholine by ion-exchange resin is proposed, and the ion-exchange resin can be regenerated and reused, has low cost, and is more suitable for large-scale industrial preparation. At present, there are no reports on this aspect at home and abroad. article

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  • Method of separating and purifying phosphatidyl choline from phospholipid by resin chromatography method
  • Method of separating and purifying phosphatidyl choline from phospholipid by resin chromatography method
  • Method of separating and purifying phosphatidyl choline from phospholipid by resin chromatography method

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Experimental program
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Effect test

Embodiment 1

[0036] The screening of embodiment 1 resin

[0037] The adsorption capacity of phosphatidylcholine per unit mass of resin is an important parameter for equipment design. The adsorption performance of the resin is closely related to the polarity and size of the adsorbed molecules, as well as the polarity, specific surface area, and pore size of the adsorbent. 10 kinds of ion exchange resins were selected, and their static adsorption to phosphatidylcholine is shown in Table 1.

[0038] It can be seen from Table 1 that 201×7, 201×4, and D296 have higher adsorption rates, followed by D113-IIIFC, 001×7, and SQD-88, while the adsorption rates of D001, SQD-61, D301-III, and D318 smaller. According to the requirements of industrial applications, six kinds of resins with high adsorption rates, 201×7, 201×4, D296, D113-IIIFC, 001×7, and SQD-88, were selected for static desorption experiments. 100% ethanol was used for desorption, and the desorption test results are shown in Table 2. ...

Embodiment 2

[0043] The influence of embodiment 2 sample solution concentration on adsorption effect

[0044] The dynamic adsorption experiment was carried out on alcohol-soluble phospholipid solutions with different concentrations, and the results are shown in Figure 1. It can be seen from Figure 1 that when the alcohol-soluble phospholipid concentration is 3-5 mg / mL, the adsorption rate of the resin to PC is relatively ideal. When the alcohol-soluble phospholipid concentration is 3mg / mL, the adsorption rate of the resin to PC is the largest, reaching 42.97%, so the best sample concentration is 3mg / ml.

Embodiment 3

[0045] Example 3 Influence of sample liquid pH on adsorption effect

[0046] Adsorption experiments were carried out at different pH values, and the results are shown in Figure 2. The results show that the adsorption effects of PC and PE are relatively ideal under weakly alkaline conditions. When the pH value of the sample solution is about 7.5, that is, without changing the pH of the original solution, the adsorption rate of D113-IIIFC resin to PC is the largest. Continue to increase the pH, the adsorption rate decreased significantly. This is because the resin itself is a weakly acidic cation exchange resin, which is more conducive to ion exchange between PC and PE under weakly alkaline conditions, but when the pH is too high, due to a large amount of sodium ions and PC (or PE) Competitive adsorption, while too much alkalinity is not good for the resin itself, resulting in a decrease in the adsorption rate. Considering the adsorption effect and the simplicity of the proces...

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Abstract

A method of using resin chromatographic analysis antigenic to purify Phosphatidyl Choline belongs to the phospholipid purification technology. The invention takes Gliadin phospholipid as raw material to prepare ethanol solution with concentration of Gliadin phospholipid 3-5mg / mL, which is add into the D113-IIIFC resin chromatographic column at the flow rate of 1mL / min, and then elutes with 95% ethanol at the rate of 0.5-1mL / min, collects the elutes, evaporates and concentrates to remove solvents, uses vacuum drying to get the pale yellow and plastic-like products of PC. In this products, the content of PC is 94.02% (wt %), and the product yield is 80.74%.

Description

technical field [0001] The invention discloses a method for separating and purifying phosphatidylcholine from phospholipids by resin chromatography, belonging to the technical field of phospholipid separation and purification. Background technique [0002] Soy lecithin is a mixture of various phospholipids, and its main components are phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidylinositol (PI), phosphatidylserine (PS) and other substances. Wherein phosphatidylcholine (phosphatidylcholine, PC), commonly known as lecithin, is a kind of natural amphoteric surfactant widely distributed in animals and plants, its chemical structural formula is as follows, it is nontoxic, non-irritating, easy biodegradation, Has special physiological effects. Different phospholipids in soybean phospholipids have structural similarities. PC is more polar than other phospholipids. When pH=7, the N of PC molecule choline has a positive charge, and the O of phosphoric acid has ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07F9/10
Inventor 刘元法王兴国金青哲汪燕
Owner JIANGNAN UNIV
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