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Process for preparing high purity soy phoshatidylcholine without lysophosphatide

A technology of phosphatidylcholine and lysophospholipid, which is applied in the field of phospholipids, can solve the problems such as failure to remove lysophospholipids and inability to realize industrialized production and the like

Inactive Publication Date: 2006-09-27
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At home and abroad, a lot of work has been done on improving the content of phosphatidylcholine in phospholipids, but most of these methods use common solvent fractionation, which can make the content of phosphatidylcholine reach 75%, and some methods use low-temperature extraction to improve the content of phosphatidylcholine report, but the phosphatidylcholine content is still below 80%. For the report using column chromatography and supercritical carbon dioxide, the former adopts a single-column multi-solvent elution system, while the latter needs to add an entrainer, and industrial production cannot be realized yet. Can not achieve the effect of removing lysophospholipids
Through literature search, there is no literature report on the industrial scale preparation of high-purity soybean phosphatidylcholine without lysophospholipids by the combination of low temperature freezing and single-solvent double-column chromatography at home and abroad

Method used

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  • Process for preparing high purity soy phoshatidylcholine without lysophosphatide
  • Process for preparing high purity soy phoshatidylcholine without lysophosphatide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Add 100 grams of powdered phospholipids to 300 milliliters of 95% ethanol, stir and leach for half an hour at room temperature, take the supernatant and let stand at -5°C for 24 hours, filter, and remove ethanol. Prepare an ethanol solution with a solid mass concentration of 2%, pass through an aluminum oxide column, and elute with 95% ethanol at 45°C. % ethanol was eluted, and the eluent was further vacuum-dried to obtain 7.5 g of the product after vacuum precipitation.

[0020] Acid value (mg / g)

Embodiment 2

[0022] Add 100 grams of powdered phospholipids to 700 milliliters of 80% methanol, stir and leach for half an hour at room temperature, take the supernatant and concentrate to half of the original volume, stand at -6°C for 20 hours, filter, and remove methanol. Prepare a methanol solution with a solid mass concentration of 2%, pass through a manganese dioxide column, and elute with 80% methanol at 20°C. After concentrating the eluent 10 times, pass through a diatomite column, 80% ethanol was eluted, and the eluent was further vacuum-dried after vacuum precipitation to obtain 9.2 g of the product.

Embodiment 3

[0024] Add 100 grams of powdered phospholipids to 1100 milliliters of 90% propanol, stir and leach for half an hour at room temperature, take the supernatant and concentrate to one-third of the original volume, stand at -8°C for 15 hours, filter, and remove the solvent. Prepare a propanol solution with a solid mass concentration of 2%, pass through an aluminum oxide column, and elute with 90% propanol at 50° C., concentrate the eluate by 10 times, pass through an attapulgite column, It was eluted with 90% propanol at °C, and the eluent was vacuum-precipitated and further vacuum-dried to obtain 4.7 g of the product.

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Abstract

The invention discloses a high-purity soya bean phosphatidyl choline without lysophosphatide preparing method in the phosphatide technique domain, which comprises the following steps: using soybean podwer phosphatide for raw material; extracting by dissolvant; freezing raffinate; treating liquor by dual column chromatogram; eluting for treating column 1; dislodging phosphatidyl ethanolamine; eluting and treating column2; dislodging lysophosphatide, wherein eluent of dissolvant,column 1 and column 2 for extracting is common single solvent; the production has no lysophosphatide; the bilineurine content of soya bean lecithin is more than 90%.

Description

technical field [0001] The invention discloses a preparation method of high-purity soybean phosphatidylcholine without lysophospholipid, which belongs to the field of phospholipid technology. Background technique [0002] Soy phospholipid is a by-product of vegetable oil processing. It is a mixture of various phospholipids, mainly including phosphatidylcholine, phosphatidylethanolamine, phosphatidylinositol and phosphatidylserine. Different processing methods of phospholipids make the obtained phospholipids have different compositions, and thus have different phospholipid applications and processing properties. It has recently been found that soybean phosphatidylcholine without lysophospholipids in soybean phospholipids has important biological functions and is more and more widely used in the pharmaceutical industry, so the preparation of high-purity soybean phosphatidylcholine without lysophospholipids has very important practical significance. At home and abroad, a lot ...

Claims

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Application Information

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IPC IPC(8): C07F9/10
Inventor 曹栋史苏佳王兴国
Owner JIANGNAN UNIV
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