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Method for simultaneous determination of nine sulfonamide antibiotics on suspended particles in water

A technology for suspended particulate matter and sulfonamides, which is applied in measuring devices, instruments, scientific instruments, etc., can solve the problems of unreported analysis methods of sulfonamide antibiotics and low content of sulfonamide antibiotics, and achieves the effect of short processing time and simple steps.

Active Publication Date: 2019-10-15
INST OF ANALYSIS GUANGDONG ACAD OF SCI (CHINA NAT ANALYTICAL
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Problems solved by technology

[0004] However, due to the structural characteristics of sulfonamide antibiotics, the content of sulfa antibiotics in suspended particles in water is extremely low, and there is no report on the analysis method for sulfonamide antibiotics on suspended particles in water.

Method used

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  • Method for simultaneous determination of nine sulfonamide antibiotics on suspended particles in water
  • Method for simultaneous determination of nine sulfonamide antibiotics on suspended particles in water
  • Method for simultaneous determination of nine sulfonamide antibiotics on suspended particles in water

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Embodiment 1

[0029] A method for simultaneous determination of 9 sulfonamide antibiotics on suspended particulates in surface water. The specific implementation steps are as follows:

[0030] Step 1. Take 10L of the target water sample, filter it with 0.45μm microporous fiber filter membrane, collect the particulate matter retained on the filter membrane and the surface after filtration, and add 200μL of internal standard substance (deuterated sulfadiazine and deuterated sulfadiazine Oxypyrimidine, dilute its concentration to 1mg / L with methanol, add 100μL respectively), freeze-dried and cut it into a centrifuge tube, add an appropriate amount of acetonitrile solution containing 1vol.% formic acid as the extractant, and shake at constant temperature (constant oscillation temperature After 3~4h, the ultrasonic wave (using an ultrasonic cleaner, power 500W) ultrasonic for 10~20min and strong ultrasound (using an ultrasonic cell disruptor, power 280W, the gap time / ultrasonic time is 3S / 1S) and 5...

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Abstract

The invention discloses a method for simultaneous determination of nine sulfonamide antibiotics on suspended particles in water. The method comprises the following steps: taking a target water sample,filtering, and collecting a filtered membrane and particles retained on the surface; adding a quantitative internal standard substance; cutting and placing in a centrifuge tube after freeze drying; adding an appropriate amount of extractant; performing constant temperature oscillation, ultrasonic treatment and centrifuging; transferring supernatant to a K-D concentration bottle; purging the extraction liquid to less than 1ml by nitrogen gas; filtering with an organic microporous filter membrane; and measuring with an ultra-high performance liquid chromatography-triple quadrupole tandem mass spectrometer. The method has the advantages of short sample pretreatment time, simple steps, simultaneous determination of the contents of nine sulfonamide antibiotics such as sulfadiazine, sulfapyridine, sulfamethazine, sulfisomidine, sulfachlorpyridazine, sulfamerazine, sulfamethoxazole, sulfaquinoxaline and trimethoprim adsorbed on the suspended particles in water, and more accurate and true reflection of the contents of the sulfonamide antibiotics in water more.

Description

Technical field: [0001] The invention relates to the technical field of water environment detection, in particular to a method for simultaneously determining 9 kinds of sulfonamide antibiotics on suspended particles in water. Background technique: [0002] Sulfonamide antibiotics are derivatives with p-aminobenzenesulfonamide structure. They are one of the most widely used antibiotics. They have a broad antibacterial spectrum and have inhibitory effects on most gram-positive and gram-negative bacteria. Due to the widespread use of sulfonamide antibiotics and incomplete absorption and metabolism in the target organisms, they continue to enter the environment and produce a series of metabolism and degradation products through processes such as adsorption, hydrolysis, photodegradation and biodegradation, and these products often have Greater toxicity. [0003] At present, antibiotics, including sulfonamide antibiotics, have been continuously detected in different water bodies in my c...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06G01N30/14
CPCG01N30/02G01N30/06G01N30/14G01N2030/062G01N2030/146
Inventor 宋玉梅吴锐张飞荀合郭鹏然
Owner INST OF ANALYSIS GUANGDONG ACAD OF SCI (CHINA NAT ANALYTICAL
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