Method for detecting antibiotic in environment sample

Abstract

The invention relates to the technical field of environment detection and is suitable for measuring erythrocin, chloramphenicol, sulfamethoxazole, sulfadiazine, roxithromycin, cefotiam, pyridazol, norfloxacin, ofloxacin, tetracycline and doxycycline in sewage, surface water and bottom mud. After being sampled, a water sample and a mud sample are pretreated and detected through ultra-high performance liquid chromatography-triple quadrupole mass spectrometry, and qualitative diagnosis is conducted through the characteristic ion pair (m/z) of a target compound, a standard curve is drawn accordingto the response value and the corresponding concentration, and the external standard method is used in quantification, so that the contents of various antibiotics in the sample are obtained. The method is accurate, sensitive and simple, and is suitable for measuring the contents of 11 typical antibiotics in the sewage, the surface water and the mud at the same time.

Description

technical field [0001] The invention relates to the technical field of detection, in particular to a detection method for antibiotics in environmental samples. Background technique [0002] Antibiotics are a class of secondary metabolites with anti-pathogenic or other active substances produced by microorganisms (including bacteria, fungi, actinomycetes) or other higher animals and plants during their life. Chemical substances with developmental functions are mainly divided into β-lactams, aminoglycosides, tetracyclines, quinolones, macrolides, etc. Antibiotics are widely used for the protection of human and animal health, or as food additives to increase the growth rate of animals. In 2013, China's antibiotic consumption reached 162,000 tons, accounting for about half of the world's consumption, of which 52% was for animal use, and 48% was for human use. More than 50,000 tons of antibiotics were discharged into the soil and water environment. In 2013, the daily consumptio...

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Problems solved by technology

[0006] According to the supervisory monitoring results of the Chengdu State-controlled Sewage Treatment Plant in the second quarter of 2016, the existing sewage treatment capacity in the downtown area of ​​Chengdu is 1.66 million tons per day, and the daily discharge to the environment is about 1.4 million tons. After the treated sewage enters the environment, trace amounts of refractory antibiotics contained in it will continue to circulate and accumulate, resulting in greater harm to the environment

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