Method for simultaneously screening multiples categories of drug residues in fish by using ultra performance liquid chromatography-quadrupole rod time-of-flight mass spectrometry

Abstract

The invention relates to a method for simultaneously screening multiples categories of drug residues in fish by using ultra performance liquid chromatography-quadrupole rod time-of-flight mass spectrometry. The multiples categories of drugs screened simultaneously comprise enrofloxacin, danofloxacin, tetracycline, oxytetracycline , chlortetracycline, doxycycline, chloramphenicol, thiamphenicol, florfenicol, sulfamethoxazole, sulfamethoxazole, sulfathiazole, sulfadiazine, sulfadoxine, sulfisoxazole, sulfaphenirazole, sulfacetamide, sulfamethazine, azithromycin, tilmicosin, medimycin, roxithromycin, acetylspiramycin, doramectin, sudan I, sudan II, sudan III, sudan IV and rhodamine B. By optimizing the parameters of a d-SPE pretreatment method, all target drugs can obtain good recovery rates.29 kinds of target drugs are simultaneously screened by an optimized chromatographic mass spectrometry condition, thereby achieving simultaneous screening of common multiples categories of drug residues in fish. The method has a good application prospect in the field of fish food.

Description

technical field [0001] The invention belongs to the technical field of food safety detection, and specifically relates to a method for simultaneously screening multi-category drug residues in fish by ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry. Background technique [0002] Fish and fish products are one of the main dietary sources, and they are also the types with a high incidence of food safety incidents. In the past two years, problems have occurred frequently. The "China Food Safety Development Report 2016" pointed out that 13 kinds of aquatic products such as shrimp and fish have safety problems. The pass rate has been lower than 96% for three consecutive years, and the safety factor needs to be improved. The primary problem in the quality and safety of fish food is excessive drug residues. In recent years, some unscrupulous merchants have not followed the NY 5071-2002 "Usage Guidelines for Pollution-Free Food and Fishery Dr...

AI Technical Summary

Problems solved by technology

In recent years, some unscrupulous merchants have not followed the NY 5071-2002 "Usage Guidelines for Pollution-Free Food and Fishery Drugs" issued by the Ministry of Agriculture in the process of increasing fisheries, neglected the ecological environment of water areas, and put in feed and used fishery drugs. , there are problems such as abuse, blindly increasing the dosage or the time of medication, which will lead to the deterioration of water quality, leading to various diseases, and then illegal medication again, forming a vicious circle

[0003] At present, the common drug residues mainly include antibiotics such as quinolones, tetracyclines, macrolides, sulfonamides, and chloramphenicol, and pigment drugs such as Sudan red and rhodamine. These drugs will eventually be passed on to humans and animals through the food chain , thereby affecting human health, leading to a decline in body immunity, damage to the nervous system, endocrine and reproductive systems, etc.; at the same time affecting the production and import and export of aquatic products, resulting in a substantial decline in product production; it will also affect the natural environment such as soil, water and air, Therefore, it is necessary to screen common drug residues in fish. However, most of the prior art is single-drug screening, lacking the simultaneous screening of enrofloxacin, dafloxacin, tetracycline, oxytetracycline, aureomycin, Doxycycline, chloramphenicol, thiamphenicol, florfenicol, sulfamethiadiazole, sulfamethoxypyridazine, sulfathiazole, sulfadiazine, sulfadoxine, sulfisoxazole, sulfabenzopyrazole, Sulfacetamide, sulfamethazine, azithromycin, tilmicosin, midecamycin, roxithromycin, acetylspiramycin, doramectin, Sudan red Ⅰ, Sudan red Ⅱ, Sudan red Ⅲ, Sudan red Method for 29 drugs in 6 categories including IV and Rhodamine B

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