Preparation method and application of natural alpha fetoprotein

The method of preparing immunoaffinity columns for alpha-fetoprotein (AFP) purification via antigen-antibody reaction solves the problems of low recovery rate and low purity in existing technologies, achieving the preparation of high-purity AFP that meets the requirements for clinical diagnosis and quality control.

CN121779554APending Publication Date: 2026-04-03ZHENGZHOU IMMUNO BIOTECH
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Patent Information

Application Number
CN202511994282.6
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-12-26
Publication Date
2026-04-03

AI Technical Summary

Technical Problem

Existing methods for separating alpha-fetoprotein result in low recovery rates and low purity, making it difficult to meet the needs of clinical diagnostics and quality control products.

Method used

An immunoaffinity purification method based on antigen-antibody reaction was adopted. An immunoaffinity column was prepared using the antibody AFP 20# that specifically binds to alpha-fetoprotein (AFP) and used to purify AFP from serum, tissue, and cell supernatants. High-purity AFP was obtained by combining SDS-PAGE and HPLC detection.

Benefits of technology

The preparation of high-purity alpha-fetoprotein was achieved with high recovery rate. Its stability and matrix effect meet the requirements for use as quality control and calibrators, providing reliable clinical diagnostic evidence.

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Abstract

The invention relates to the technical field of antigens and antibodies, in particular to a preparation method and application of natural alpha-fetoprotein, the natural alpha-fetoprotein is secreted and expressed by tumor cells HUH-7, cultured by a hollow fiber column and purified by immunoaffinity purification, amino acid sequences of an antibody AFP 20 # light chain variable region and a heavy chain variable region used for coupling NHS filler are shown as SEQ ID NO: 1-2, and the amino acid sequences of the antibody AFP 20 # light chain variable region and the heavy chain variable region are shown as SEQ ID NO: 1-2. The antibody can specifically recognize the alpha fetoprotein, the alpha fetoprotein purified by the antibody is high in recovery rate and purity, the protein stability, the matrix effect and the reaction rate can meet the use requirements of a calibration product and a quality control product, and the antibody makes a contribution to the application of the alpha fetoprotein in the in-vitro diagnosis industry.
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