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Method for simultaneously determining multiple polyphenolic compound content of fruit

A technology of polyphenol compounds and multiple polyphenols, applied in measuring devices, instruments, scientific instruments, etc., can solve the problems of difficult and accurate measurement, complicated methods, low polyphenol content, etc., and achieve the improvement of measurement accuracy and recovery rate, Protect the chromatographic column, good reproducible effect

Inactive Publication Date: 2008-02-20
FUDAN UNIV
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Problems solved by technology

However, the types of polyphenols currently studied are quite different, and they are limited to a small number of fruits such as apples, grapes, and citrus.
In addition, the juicing method is usually used to prepare fruit samples. Although the juicing method is very simple, it is difficult to measure accurately because the water content in the sample is too high and the polyphenol content is too low, and the extraction efficiency of some polyphenols is low.
Many fruit juices cannot be directly used for measurement, and the lipids, carotenoids and chlorophyll contained in them need to be removed, and the polyphenols in some fruit juices need to be hydrolyzed before measurement, and the method is more complicated

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  • Method for simultaneously determining multiple polyphenolic compound content of fruit
  • Method for simultaneously determining multiple polyphenolic compound content of fruit

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Embodiment Construction

[0028] Below in conjunction with embodiment further illustrate the present invention.

[0029] 1. Preparation of reference solution:

[0030] Standard samples of gallic acid, protocatechin, catechin, epicatechin, caffeic acid, rutin, chlorogenic acid, and ferulic acid with a purity of ≥99% were taken respectively. A standard stock solution of about 1 g / L of the above eight polyphenols was prepared with 10% methanol solution. According to the actual content of polyphenols in the fruit and the response value of each polyphenol at a certain ultraviolet detection wavelength, dilute a certain number of times to prepare a mixed standard solution containing the above 8 kinds of polyphenol compounds, and then dilute the above solution into different concentration gradients , that is, 0.2, 0.4, 0.6, 0.8, 1.0 times the concentration of the above mixed standard solution.

[0031] 2. Preparation of the test solution:

[0032] Microwave extraction method sample preparation: fresh fruit ...

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Abstract

The utility model discloses a high efficiency liquid phase chromatogram method which can detect the content of various polyphenolic compounds inside fruits at the same time by adopting liquid phase chromatogram containing ultraviolet detector and reversed-phase bonded silica chromatographic column; organic solvent mobile phase A and acidic aqueous mobile phase B with certain Ph value are adopted as gradient eluant. The utility model quantitatively detects various polyphenolic compounds inside fruits at the same time only on normal high efficiency liquid phase chromatogram. The utility model builds a method of detecting the content of various polyphenolic compounds inside fruits which combines microwave extraction and high efficiency liquid phase chromatogram. The utility model has the advantages of advanced and accurate method, simple and quick sample processing; meanwhile, Carrez reagent is used as scavenging agent of fruits sample and is firstly used as fruit polyphenols testing.

Description

technical field [0001] The invention relates to a method for determining the content of polyphenolic compounds in fruits. Using a common high-performance liquid chromatograph and specific chromatographic conditions, it is possible to complete the determination of gallic acid, protocatechuic acid, catechin, green elemental acid, and catechin in fruits. Determination of polyphenolic compounds such as tea, caffeic acid, ferulic acid or rutin with accurate results. Background technique [0002] Fruit polyphenols have medicinal and edible value. Due to the unique chemical and biological characteristics of polyphenols, polyphenols are extracted from edible plants and purified. They can be used as natural food additives, adjust food flavor, and play an antioxidant role ( Efficient, non-toxic and health-care) and antiseptic. Polyphenolic compounds in fruits not only have medicinal and edible value, but also one of the important indicators of fruit maturity. The commonly used metho...

Claims

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Application Information

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IPC IPC(8): G01N30/89
Inventor 张晋芬袁冰徐华龙沈伟
Owner FUDAN UNIV
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