Phycocyanin microcapsule and preparation method of phycocyanin microcapsule

A phycocyanin and microcapsule technology, applied in the field of food biology, can solve the problems of light, heat and acid-base sensitivity, limited development and application, poor stability, etc., and achieves the effect of simple method, low professional quality requirements, and improved stability.

Inactive Publication Date: 2012-08-22
YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, phycocyanin has broad application prospects and strong market economic value, but its large-scale development and application are limited due to its sensitivity to light, heat, acid and alkali, poor stability and easy inactivation.

Method used

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  • Phycocyanin microcapsule and preparation method of phycocyanin microcapsule
  • Phycocyanin microcapsule and preparation method of phycocyanin microcapsule
  • Phycocyanin microcapsule and preparation method of phycocyanin microcapsule

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Preparation of phycocyanin microcapsules:

[0027] Dissolving sodium alginate in water to prepare a sodium alginate solution, making the final concentration 4-6%; dissolving phycocyanin in water to prepare a phycocyanin solution, making the final concentration 5-9%;

[0028] Mix and homogenize the above sodium alginate solution and phycocyanin solution in equal volumes, set aside;

[0029] Then adopt extrusion equipment to extrude the above-mentioned homogeneous solution to the CaCl of 2-3% (mass percentage) by extrusion method 2 In the solution, stand and solidify for 30-60min;

[0030] After the above-mentioned solidification is washed with water, it is transferred to a chitosan solution with a concentration of 1.5-2.5% prepared with 1% acetic acid solution, and the coating is shaken for 60-120 minutes; then washed with water, filtered, and freeze-dried to obtain microcapsules.

[0031] The drying process is as follows: put the microcapsules into a petri dish, freez...

Embodiment 2

[0033] Preparation of phycocyanin microcapsules:

[0034] Sodium alginate solution was prepared by dissolving sodium alginate in water so that its final concentration was 4%; phycocyanin was prepared by dissolving phycocyanin in water so that its final concentration was 5%;

[0035] Mix and homogenize the above sodium alginate solution and phycocyanin solution in equal volumes, set aside;

[0036] Then the above-mentioned homogeneous solution is extruded to 2% CaCl by extrusion using an extruder 2 In the solution, let stand to solidify for 30min;

[0037] After the above-mentioned solidification is washed with water, it is transferred to a chitosan solution prepared with a concentration of 2% and 1% acetic acid solution, and the film is shaken for 90 minutes; then washed with water, filtered, and freeze-dried to obtain microcapsules.

[0038] The drying process is as follows: put the microcapsules into a petri dish, freeze overnight in a refrigerator at -20°C, put them into ...

Embodiment 3

[0048] Preparation of phycocyanin microcapsules:

[0049] Sodium alginate solution was prepared by dissolving sodium alginate in water so that its final concentration was 5%; phycocyanin was prepared by dissolving phycocyanin in water so that its final concentration was 9%;

[0050] Mix and homogenize the above sodium alginate solution and phycocyanin solution in equal volumes, set aside;

[0051] Then the above-mentioned homogeneous solution is extruded to 3% CaCl by extrusion using an extruder 2 In the solution, stand and solidify for 60 minutes;

[0052]After the above-mentioned solidification is washed with water, it is transferred to a chitosan solution prepared with a concentration of 1.5% and 1% acetic acid solution, and the membrane is shaken for 60 minutes; then washed with water, filtered, and freeze-dried to obtain microcapsules.

[0053] The drying process is as follows: put the microcapsules into a petri dish, freeze overnight in a refrigerator at -20°C, put the...

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Abstract

The invention relates to the technical field of food bioscience, in particular to a phycocyanin microcapsule and a preparation method of the phycocyanin microcapsule. The phycocyanin microcapsule consists of wall materials and core materials, wherein the wall materials are algin and chitosan, and the core materials are phycocyanin. The phycocyanin microcapsule obtained in the invention realizes the 100 percent water solubility, and the encapsulation efficiency is 50 to 60 percent, the stability of the phycocyanin is improved, and in addition, the physiological function of the phycocyanin can be enhanced through the microcapsule slow release effect. The preparation method has the advantages that the operation is simple, the used raw materials are raw materials permitted in the food field, a new thought is provided for the occurrence of the phycocyanin in various product forms, and in addition, the diversified requirements of customers are favorably met.

Description

technical field [0001] The invention relates to the field of food biotechnology, in particular to a phycocyanin microcapsule and a preparation method thereof. Background technique [0002] Phycocyanin is a kind of photosynthetic auxiliary pigment commonly found in cyanobacteria cells. It is a special pigment protein composed of open-chain tetrapyrrole compound-phycobilin and apoprotein covalently cross-linked through thioether bonds. become. Phycocyanin is mainly found in spirulina, where the content is as high as 10-20%. Phycocyanin can be used as a natural pigment and is widely used as a colorant for food and cosmetics. At the same time, phycocyanin is also a rare natural fluorescent pigment protein that exists in nature. It can be made into fluorescent reagents, fluorescent probes, and fluorescent tracers. Markers are used in the fields of clinical medical diagnosis, immunochemistry and bioengineering. Other studies have shown that phycocyanin has physiological functio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L1/305
Inventor 闫鸣艳秦松刘冰焦绪栋
Owner YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI
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