Preparation method of carbon nanotube-chitosan-phycocyanin nanoparticles

A technology of phycocyanin and carbon nanotubes, which can be applied to medical preparations containing non-active ingredients, medical preparations containing active ingredients, and pharmaceutical formulas, etc., and can solve problems such as adverse reactions and easy accumulation in cells, organs, and tissues , achieving good stability, improved biocompatibility, and easy mass production

Inactive Publication Date: 2011-12-14
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] However, after entering the organism, CNTs in the original state cannot be dissolved in physiological fluids, but are easily aggregated in cells, organs and tis...

Method used

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  • Preparation method of carbon nanotube-chitosan-phycocyanin nanoparticles

Examples

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Embodiment 1

[0037] The preparation method of biomedical material carbon nanotube-chitosan-phycocyanin nanoparticle, the steps are as follows:

[0038] (1) Weigh 40mg of carboxylated carbon nanotube powder with a diameter of 10nm (carboxylation rate 1wt%), add it to 150ml 5mM PBS solution, ultrasonically disperse for 60 minutes, then add 170mg water-soluble chitosan CS, and continue ultrasonic oscillation After 60 minutes, the mixed solution was magnetically stirred for 1 hour after sonication. After standing and stratifying for 6 hours, take the upper layer liquid and centrifuge at a high speed of 10000r / min. The upper layer liquid after centrifugation is the intermediate sample: chitosan-modified water-soluble multi-walled carbon nanotube homogeneous solution (MWCNT-CS) . Use a 30kD ultrafiltration tube to remove excess CS in MWCNT-CS, and freeze-dry the resulting filter cake as a sample for characterization analysis;

[0039] (2) Add 70 mg of carbodiimide hydrochloride (EDC-HCl) and 1...

Embodiment 2

[0050] The preparation method of biomedical material carbon nanotube-chitosan-phycocyanin nanoparticle, the steps are as follows:

[0051] (1) Weigh 20mg of carboxylated carbon nanotube powder with a diameter of 20nm (carboxylation rate 5wt%), add it to 120ml 5mM PBS solution, ultrasonically disperse for 60 minutes, then add 130mg water-soluble chitosan CS, and continue ultrasonic oscillation After 40 minutes, the mixed solution was magnetically stirred for 70 minutes after sonication. After standing and stratifying for 8 hours, take the upper layer liquid and centrifuge at a high speed of 10000r / min. The upper layer liquid after centrifugation is the intermediate sample modified by chitosan: homogeneous solution of water-soluble multi-walled carbon nanotubes (MWCNT-CS) . Use a 20kD ultrafiltration tube to remove excess CS in MWCNT-CS, and freeze-dry the resulting filter cake as a sample for characterization analysis;

[0052] (2) Add 70 mg of carbodiimide hydrochloride (EDC...

Embodiment 3

[0054] The preparation method of biomedical material carbon nanotube-chitosan-phycocyanin nanoparticle, the steps are as follows:

[0055] (1) Weigh 30mg of carboxylated carbon nanotube powder with a diameter of 20nm (carboxylation rate 10wt%), add it to 150ml 5mM PBS solution, ultrasonically disperse for 40 minutes, then add 100mg of water-soluble chitosan, and continue ultrasonic oscillation for 45 minutes Minutes, the mixed solution was magnetically stirred for 0.5 hours after sonication. After standing still for 10 hours, take the upper layer liquid and centrifuge at a high speed of 10000r / min. The upper layer liquid after centrifugation is the intermediate sample modified by chitosan: a homogeneous solution of water-soluble multi-walled carbon nanotubes (MWCNT-CS). Use a 30kD ultrafiltration tube to remove excess chitosan in MWCNT-CS, and freeze-dry the resulting filter cake as a sample for characterization analysis;

[0056](2) Add 50 mg of carbodiimide hydrochloride (E...

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Abstract

The invention provides a preparation method of carbon nanotube-chitosan-phycocyanin nanoparticles, which comprises the following steps: modifying carboxylated carbon nanotubes with water-soluble chitosan in a non-covalent mode to obtain a water-soluble multiwall carbon nanotube-chitosan (MWCNT-CS) compound; and coupling the MWCNT-CS compound with high-purity spirulina phycocyanin to obtain the biomedical material carbon nanotube-chitosan-phycocyanin nanoparticles (MWCNT-CS-PC). The method has the advantage of simple preparation technique; and the obtained biomedical material has the advantages of favorable water solubility and biocompatibility, has favorable absorption characteristic in both visible light and near-infrared light regions, and is expected to be applied to photodynamic therapy and photo-thermal therapy on cancer cells.

Description

technical field [0001] The invention relates to a preparation method of a carbon nanotube composite material, in particular to a preparation method of a carbon nanotube-chitosan-phycocyanin nanoparticle. Background technique [0002] Phycocyanin (Phycocyanin, hereinafter referred to as PC) is a light-harvesting pigment protein commonly found in cyanobacteria, red algae, cryptophyta and a few dinoflagellates, and its content in spirulina is as high as 7% to 20%. As a natural pigment protein with no toxic side effects, PC can replace synthetic dyes in food, cosmetics and medicine. Studies have shown that PC has anti-tumor activity and can improve immune function. In particular, PC can produce a photodynamic killing effect on human cancer cells after being irradiated with a laser in the visible light region, that is, it has the potential of photodynamic therapy (PDT). [0003] Carbon nanotubes (hereinafter referred to as CNTs) are seamless hollow carbon tubes formed by curlin...

Claims

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Application Information

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IPC IPC(8): A61K41/00A61K47/48A61P35/00
Inventor 张学武廖晓霞
Owner SOUTH CHINA UNIV OF TECH
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