Preparation and application of phycocyanin extract
A technology of phycocyanin and extract, which is applied in the field of medicine, can solve the problems of cumbersome operation and difficulty in large-scale production, and achieve the effects of good water solubility, good tumor inhibition effect, and convenient administration
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Embodiment 1
[0022] preparation:
[0023] Weigh 10 g of commercially available spirulina algae powder (Shenzhen Biotechnology Co., Ltd.), suspend in 150 mL of water, stir and mix well, freeze and thaw three times at -20-4°C, break the cell wall of spirulina, and obtain a crude extract. The obtained crude extract was centrifuged at 9000 rpm for 10 min to obtain the supernatant. About 44 g of ammonium sulfate was added to the supernatant, stirred thoroughly to dissolve, and after standing at 4°C for 4 hours, centrifuged at 9,000 rpm for 10 minutes to discard the supernatant. After the obtained precipitate was dissolved in 30 mL of pure water, the crude protein extract was purified by two-phase extraction, wherein the two-phase extraction condition was a 1.2 kg two-phase system, and the mass fractions of each component were 4% PEG, 15% Potassium chloride, 10% potassium dihydrogen phosphate-dipotassium hydrogen phosphate aqueous solution with a pH of 7.0, fully shaken, and left to stand at 40...
Embodiment 2
[0032]Effect experiment of the phycocyanin extract obtained by the above extraction on the transplanted S 180 sarcoma mice:
[0033] SPF Kunming mice, 18-22 g, male, were provided by the Animal Center of Qingdao Institute of Drug Control. The S 180 sarcoma tumor strain was transferred from the Chinese Academy of Medical Sciences, and passed down in our laboratory. Under aseptic operation, 0.2ml of tumor cell suspension was subcutaneously inoculated into the right anterior armpit of each mouse (the number of tumor cells contained in the microscopic examination was 9×10 7 pieces / ml). The day after inoculation of tumor cells, mice were randomly divided into 5 groups according to body weight, 8 mice in each group, and administered 24 hours after inoculation. Drugs were dissolved in normal saline for intragastric or intraperitoneal injection in mice, and the volume was 0.1ml / 10g according to body weight. The negative control group was intragastrically administered normal saline, ...
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