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Preparation and application of phycocyanin extract

A technology of phycocyanin and extract, which is applied in the field of medicine, can solve the problems of cumbersome operation and difficulty in large-scale production, and achieve the effects of good water solubility, good tumor inhibition effect, and convenient administration

Inactive Publication Date: 2010-11-24
INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

These two methods are cumbersome to operate and difficult to achieve large-scale production

Method used

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  • Preparation and application of phycocyanin extract
  • Preparation and application of phycocyanin extract
  • Preparation and application of phycocyanin extract

Examples

Experimental program
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Effect test

Embodiment 1

[0022] preparation:

[0023] Weigh 10 g of commercially available spirulina algae powder (Shenzhen Biotechnology Co., Ltd.), suspend in 150 mL of water, stir and mix well, freeze and thaw three times at -20-4°C, break the cell wall of spirulina, and obtain a crude extract. The obtained crude extract was centrifuged at 9000 rpm for 10 min to obtain the supernatant. About 44 g of ammonium sulfate was added to the supernatant, stirred thoroughly to dissolve, and after standing at 4°C for 4 hours, centrifuged at 9,000 rpm for 10 minutes to discard the supernatant. After the obtained precipitate was dissolved in 30 mL of pure water, the crude protein extract was purified by two-phase extraction, wherein the two-phase extraction condition was a 1.2 kg two-phase system, and the mass fractions of each component were 4% PEG, 15% Potassium chloride, 10% potassium dihydrogen phosphate-dipotassium hydrogen phosphate aqueous solution with a pH of 7.0, fully shaken, and left to stand at 40...

Embodiment 2

[0032]Effect experiment of the phycocyanin extract obtained by the above extraction on the transplanted S 180 sarcoma mice:

[0033] SPF Kunming mice, 18-22 g, male, were provided by the Animal Center of Qingdao Institute of Drug Control. The S 180 sarcoma tumor strain was transferred from the Chinese Academy of Medical Sciences, and passed down in our laboratory. Under aseptic operation, 0.2ml of tumor cell suspension was subcutaneously inoculated into the right anterior armpit of each mouse (the number of tumor cells contained in the microscopic examination was 9×10 7 pieces / ml). The day after inoculation of tumor cells, mice were randomly divided into 5 groups according to body weight, 8 mice in each group, and administered 24 hours after inoculation. Drugs were dissolved in normal saline for intragastric or intraperitoneal injection in mice, and the volume was 0.1ml / 10g according to body weight. The negative control group was intragastrically administered normal saline, ...

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Abstract

The invention belongs to the technical field of medicament, and particularly relates to preparation and application of phycocyanin extract. The phycocyanin extract is prepared by the following steps of: crushing spirulina cell walls of spirulina powder to obtain a crude extract by a repeated freeze-thawing method at the temperature of between 20 DEG C below zero and 4 DEG C; depositing the crude extract to obtain a crude protein extract by using 50 to 60 mass percent of ammonium sulfate; and then extracting and purifying the crude protein extract by adopting double aqueous phases, and removing salt by using excess infiltration to obtain phycocyanin extract solution. The phycocyanin extract mainly comprises phycocyanin, and the aqueous solution of the phycocyanin extract has the absorbance characteristics that A620 / 280 is slightly equal to 3.0+ / -0.2 and A620 / 650 is slightly equal to 2.2+ / -0.1. The phycocyanin extract is obtained by mainly combining the processes of ammonium sulfate deposition, double aqueous phase extraction, dialysis and salt removal and the like; and the phycocyanin extract administrated to S180 sarcoma mice with different dosages shows good tumor inhibiting effect and has no obvious toxicity on the spleen and the thymus gland.

Description

technical field [0001] The invention belongs to the technical field of medicine, in particular to the preparation and application of a phycocyanin extract. Background technique [0002] Spirulina is a food macroalgae whose nutritional value has been widely reported. As the main protein composition in Spirulina, phycocyanin is composed of two apoproteins, α and β, and a chromophore. It has a maximum absorption at 620nm and a strong red fluorescence at 640nm. Based on good water solubility, high fluorescence intensity and anti-oxidation properties, phycocyanin can be used as a natural pigment additive in the food and cosmetics industry, and at the same time it can be developed into a fluorescent reagent for medical diagnosis, immunochemistry and bioengineering research fields , in addition, as an important physiologically active ingredient, it can also be made into medicine for medical care. At present, the preparation methods of phycocyanin mainly include ion exchange chrom...

Claims

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Application Information

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IPC IPC(8): C07K14/415C07K1/36C07K1/34C07K1/30C07K1/14A61K38/16A61P35/00
Inventor 陈英杰秦松李富超郭雪洁刘少芳董晓弟
Owner INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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