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Method for separating high purity phycocyanin from spirulina

A technology of phycocyanin and spirulina, which is applied in the field of separation and purification of phycocyanin, can solve problems such as the limitation of large-scale development and utilization of phycocyanin, the lack of green technology, and the sudden increase in purification costs, and achieves low price, convenient operation, short time effect

Inactive Publication Date: 2010-12-01
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

traditional method ] The main disadvantages of this method are: (1) mechanical methods such as ultrasonic or high-pressure homogenization are used to treat algae powder, which consumes a lot of energy; (2) traditional methods often require multi-step chromatography in series to obtain high-purity phycocyanin, which is not only time-consuming, but also expensive. The yield is low, and the cost of purification increases sharply due to the expensive chromatographic packing materials
The production of high-purity Spirulina phycocyanin is still in the laboratory research stage, and the lack of green technology suitable for industrial production has greatly restricted the large-scale development and utilization of phycocyanin

Method used

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  • Method for separating high purity phycocyanin from spirulina
  • Method for separating high purity phycocyanin from spirulina
  • Method for separating high purity phycocyanin from spirulina

Examples

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Effect test

Embodiment 1

[0032] The method for efficiently separating and purifying high-purity phycocyanin from spirulina, the steps are as follows:

[0033] (1) Take spirulina powder, wash it once with distilled water, then add 0.05M potassium phosphate buffer solution according to the solid-liquid mass ratio of 1:10, stir magnetically for 6 hours, centrifuge at 6000r / min for 20min at 4°C, and take the The supernatant is the crude extract of phycocyanin.

[0034] (2) Add water-soluble chitosan to the crude extract of step (1), so that its mass concentration in the phycocyanin solution is 0.1%, stir evenly, adjust the pH of the solution to 6.5, and continue stirring for 15 minutes, Centrifuge at 6000r / min for 20min at 4°C, and take the supernatant.

[0035] (3) add wooden powdery activated carbon to the supernatant of step (2) gained, make the ratio of activated carbon quality and phycocyanin solution volume be 100g / L, stir 3 minutes, centrifuge and collect supernatant, add to gained supernatant Co...

Embodiment 2

[0044] The method for efficiently separating and purifying high-purity phycocyanin from spirulina, the steps are as follows:

[0045] (1) Take spirulina powder, wash it 3 times with distilled water, then add 0.5M potassium phosphate buffer solution according to the mass ratio of solid to liquid 1:20, stir magnetically for 2 hours, centrifuge at 13000r / min for 6min at 8°C, take the The supernatant is the crude extract of phycocyanin.

[0046] (2) Add water-soluble chitosan to the crude extract of step (1), so that its mass concentration in the phycocyanin solution is 1%, stir evenly, adjust the pH of the solution to 7.5, and continue stirring for 3 minutes, Centrifuge at 13000r / min for 6min at 8°C, and take the supernatant.

[0047] (3) add wooden powdery activated carbon to the supernatant of step (2) gained, make the ratio of activated carbon quality and phycocyanin solution volume be 50g / L, stir 10 minutes, centrifuge and collect supernatant, to gained supernatant Continue...

Embodiment 3

[0051] The method for efficiently separating and purifying high-purity phycocyanin from spirulina, the steps are as follows:

[0052] (1) Take spirulina powder, wash it twice with distilled water, then add 0.2M sodium phosphate buffer solution according to the solid-liquid mass ratio of 1:15, stir magnetically for 4 hours, centrifuge at 8000r / min for 10min at 10°C, and take the The supernatant is the crude extract of phycocyanin.

[0053] (2) Add water-soluble chitosan to the crude extract of step (1), so that its mass concentration in the phycocyanin solution is 0.5%, stir evenly, adjust the pH of the solution to 6.8, and continue stirring for 5 minutes, Centrifuge at 8000r / min for 10min at 10°C, and take the supernatant.

[0054] (3) add wooden powdery activated carbon to the supernatant of step (2) gained, make the ratio of activated carbon quality and phycocyanin solution volume be 80g / L, stir 5 minutes, centrifuge and collect supernatant, to gained supernatant Continue ...

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Abstract

The invention discloses a method for separating high purity phycocyanin from spirulina, comprising the following steps: adopting 0.05-0.5M of phosphate buffer to elute a spirulina powder raw material by an elution method, enabling the phycocyanin to be effused from spirulina cells, adding soluble chitosan to phycocyanin crude extract, controlling the mass concentration of chitosan in the phycocyanin solution to be 0.1-1%, and collecting supernate by centrifuging; adding activated carbon to the supernate, centrifuging and collecting supernate, adopting ammonium sulfate precipitation with the mass concentration of 30-60% to precipitate the phycocyanin in the solution, adopting ion-exchange chromatography, and performing gradient elution by phosphate buffer containing sodium chloride to obtain the high purity phycocyanin solution. The invention has the advantages of low cost, short elapsed time, convenient and rapid operation, low energy consumption, and easy massive preparation, is an efficient method for massively preparing phycocyanin with high purity and high activity and is favor of scaled development and utilization of the phycocyanin in our country.

Description

technical field [0001] The invention relates to a method for separating phycocyanin, in particular to a method for efficiently separating and purifying phycocyanin from spirulina, and belongs to the technical field of separation and purification of phycocyanin. Background technique [0002] Phycocyanin (PC) is a light-harvesting pigment protein commonly found in cyanobacteria, red algae, cryptophyta and a few dinoflagellates. The content in spirulina is as high as 7% to 20%. 100% high efficiency transfers light energy to the light system preferentially. Phycocyanin is not only of great significance in the exploration of the primary reaction mechanism of photosynthesis, but also can be used as a fluorescent molecular probe in biomedical research. As a natural pigment protein with no toxic side effects, it can replace synthetic dyes in food, cosmetics and in medicine. Studies have shown that phycocyanin has anti-tumor activity and can improve immune function. Its characteris...

Claims

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Application Information

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IPC IPC(8): C07K14/405C07K1/18C07K1/14
Inventor 张学武廖晓霞
Owner SOUTH CHINA UNIV OF TECH
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